Oligo Synthesis : CEPs
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2"-dX-CE Phosphoramidite
2"-Deoxyxanthosine phosphoramidite
Glen Research
| Catalogue No. | Description | Pack Size | Price | Qty |
|
|---|---|---|---|---|---|
| 10-1537-02 | 2"-dX-CE Phosphoramidite | 0.25g | £560.00£475.00Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 10-1537-90 | 2"-dX-CE Phosphoramidite | 100umole | £256.00£212.80Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 10-1537-95 | 2"-dX-CE Phosphoramidite | 50umole | £136.00£114.00Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
Related products
2"-dX-CE Phosphoramidite
2"-Deoxyxanthosine phosphoramidite
Glen Research
Catalog Number: 10-1537-xx
Description: dX-CE Phosphoramidite
| 5"-(4,4"-Dimethoxytrityl)-2,6-O-bis(2-(4-nitrophenyl)ethyl)-2"-deoxyXanthosine,3Õ-[(2-cyanoethyl)-N,N-diisopropyl)]-phosphoramidite | ||
| Formula: C56H61N8O12P | M.W.: 1069.10 | F.W.: 330.19 |
Diluent: Anhydrous Acetonitrile |
| Coupling: No changes needed from standard method recommended by synthesizer manufacturer. |
| Deprotection: With the oligonucleotide still attached to the support, treat with 1 M DBU in anhydrous ACN for 2-3 minutes, followed by 17 hours using fresh 1 M DBU at room temperature.Rinse support with ACN and then deprotect as required by nucleobases. Note, the dX nucleoside is susceptible to depurination though short exposure to acid is tolerated - e.g. Glen-Pak purification. |
| Storage: Freezer storage, -10 to -30°C, dry |
| Stability in Solution: 1-2 days |
2’-DeoxyXanthosine (dX) is a naturally occurring nucleoside that may be derivedfrom oxidative deamination of 2’-deoxyGuanosine (dG). dX has a similar bondingpattern to thymidine and it may base pair with dA, with such purine-purineinteractions causing duplex distortion. dX also featured in attempts to extendthe genetic alphabet with a new base pair of dX and pyrimidine-2,4-diaminenucleoside. dX has also interested researchers in the field of DNA damage andrepair since it is a product of nitric oxide-induced mutagenesis.
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2"-dX-CE Phosphoramidite
2"-Deoxyxanthosine phosphoramidite
Glen Research
MSDS
Glen Report 22.2
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2"-dX-CE Phosphoramidite
2"-Deoxyxanthosine phosphoramidite
Glen Research
- R Eritja, et al., Nucleic Acids Res., 1986, 14, 8135-53
- M J Lutz, H A Held, M Hottiger, U Hubscher, and S A Benner, Nucleic Acids Res., 1996, 24, 1308-13
- G E Wuenschell, T R O"Connor, and J Termini, Biochemistry, 2003, 42, 3608-16
- S C Jurczyk, J Horlacher, K G Devined, S A Benner, and T R Battersby, Helv Chim Acta, 2000, 83, 1517-1524
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
2"-dX-CE Phosphoramidite
2"-Deoxyxanthosine phosphoramidite
Glen Research
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures.
| ABI 392/394 | |||||||||
| Cat.No. | PackSize | Grams/Pack | 0.1M Dil.(mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 10-1537-02 | 0.25grams | .25grams | 2.34 | 64.67 | 38.8 | 24.25 | 17.64 | 12.93 | 3.23 |
| 10-1537-90 | 100µmoles | .107grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| 10-1537-95 | 50µmoles | .053grams | .5 | 3.33 | 2 | 1.25 | .91 | .67 | .17 |
| Expedite | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 10-1537-02 | 0.25grams | .25grams | 3.49 | .07 | 63.4 | 39.63 | 28.82 | 3.96 | |
| 10-1537-90 | 100µmoles | .107grams | 1.5 | .07 | 23.6 | 14.75 | 10.73 | 1.48 | |
| 10-1537-95 | 50µmoles | .053grams | .75 | .07 | 8.6 | 5.38 | 3.91 | .54 | |
| Beckman | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 10-1537-02 | 0.25grams | .25grams | 3.49 | .07 | 65 | 40.63 | 29.55 | ||
| 10-1537-90 | 100µmoles | .107grams | 1.5 | .07 | 25.2 | 15.75 | 11.45 | ||
| 10-1537-95 | 50µmoles | .053grams | .75 | .07 | 10.2 | 6.38 | 4.64 | ||
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