Oligo Synthesis : CEPs
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2",3"-ddG-CE Phosphoramidite
2",3"-ddG-CE Phosphoramidite
Glen Research
| Catalogue No. | Description | Pack Size | Price | Qty |
|
|---|---|---|---|---|---|
| 10-7201-02 | 2",3"-ddG-CE Phosphoramidite | 0.25g | £540.00£513.00Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 10-7201-90 | 2",3"-ddG-CE Phosphoramidite | 100µmoles | £116.00£110.20Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
Related products
2",3"-ddG-CE Phosphoramidite
2",3"-ddG-CE Phosphoramidite
Glen Research
| 2",3"-ddG-CE Phosphoramidite |
chain terminators
In situations where ligation must be blocked at the 5’ terminus, 5’-OMe-dT may be used. 5’-OMe modification of a strand of siRNA using 5’-OMe-T can control guide strand selection and targeting specificity.1 5’-Amino-dT terminates an oligonucleotide with a 5’-amino group which may be used for attaching a peptide or a PNA sequence. To avoid polymerase extension at the 3’ terminus, 2’,3’-dideoxynucleoside and 3’-deoxynucleoside CPGs have proved to be effective. 2’,3’- Phosphoramidites are designed to be used with the 5’-phosphoramidites and supports. Since these phosphoramidites have no DMT group, they are not compatible with purification by the DMT-on technique. Ion exchange HPLC or PAGE should be used to purify these dideoxy terminated oligos to ensure that shorter sequences (containing 3’-OH) groups are removed. (3’-Termination can also be effected using a 3’-3’ linkage formed using 5’-supports, or 3’-spacer C3 CPG.)
Catalog Number: 10-7201-xx
Description: 2",3"-ddG-CE Phosphoramidite
| N2-dimethylaminomethylidene-2",3"-dideoxyGuanosine,5"-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite | ||
| Formula: C22H35N8O4P | M.W.: 506.54 | F.W.: 313.20 |
Diluent: Anhydrous Acetonitrile |
| Coupling: No changes needed from standard method recommended by synthesizer manufacturer. |
| Deprotection: No changes needed from standard method recommended by synthesizer manufacturer. |
| Storage: Freezer storage, -10 to -30°C, dry |
| Stability in Solution: 2-3 days |
chain terminators
In situations where ligation must be blocked at the 5’ terminus, 5’-OMe-dT may be used. 5’-OMe modification of a strand of siRNA using 5’-OMe-T can control guide strand selection and targeting specificity.1 5’-Amino-dT terminates an oligonucleotide with a 5’-amino group which may be used for attaching a peptide or a PNA sequence. To avoid polymerase extension at the 3’ terminus, 2’,3’-dideoxynucleoside and 3’-deoxynucleoside CPGs have proved to be effective. 2’,3’- Phosphoramidites are designed to be used with the 5’-phosphoramidites and supports. Since these phosphoramidites have no DMT group, they are not compatible with purification by the DMT-on technique. Ion exchange HPLC or PAGE should be used to purify these dideoxy terminated oligos to ensure that shorter sequences (containing 3’-OH) groups are removed. (3’-Termination can also be effected using a 3’-3’ linkage formed using 5’-supports, or 3’-spacer C3 CPG.)
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
2",3"-ddG-CE Phosphoramidite
2",3"-ddG-CE Phosphoramidite
Glen Research
MSDS
Glen Report
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
2",3"-ddG-CE Phosphoramidite
2",3"-ddG-CE Phosphoramidite
Glen Research
(1) P.Y. Chen, et al., RNA, 2008, 14, 263-274..
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
2",3"-ddG-CE Phosphoramidite
2",3"-ddG-CE Phosphoramidite
Glen Research
2",3"-ddG-CE Phosphoramidite
| N2-dimethylaminomethylidene-2",3"-dideoxyGuanosine,5"-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite | ||
| Formula: C22H35N8O4P | M.W.: 506.54 | F.W.: 313.20 |
Diluent: Anhydrous Acetonitrile |
| Coupling: No changes needed from standard method recommended by synthesizer manufacturer. |
| Deprotection: No changes needed from standard method recommended by synthesizer manufacturer. |
| Storage: Freezer storage, -10 to -30°C, dry |
| Stability in Solution: 2-3 days |
| Material Safety Data Sheet |
Literature Highlights
| Glen Report 13.1: NEW MONOMERS: 2,4-DIFLUOROTOLUENE, FLUORESCEIN PHOSPHORAMIDITE AND SUPPORT, 2",3"-DIDEOXYNUCLEOSIDES |
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.
| ABI 392/394 | |||||||||
| Cat.No. | PackSize | Grams/Pack | 0.1M Dil.(mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 10-7201-02 | 0.25grams | .25grams | 4.94 | 151.33 | 90.8 | 56.75 | 41.27 | 30.27 | 7.57 |
| 10-7201-90 | 100µmoles | .051grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| Expedite | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 10-7201-02 | 0.25grams | .25grams | 7.37 | .067 | 141 | 88.13 | 64.09 | 8.81 | |
| 10-7201-90 | 100µmoles | .051grams | 1.5 | .067 | 23.6 | 14.75 | 10.73 | 1.48 | |
| Beckman | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 10-7201-02 | 0.25grams | .25grams | 7.37 | .067 | 142.6 | 89.13 | 64.82 | ||
| 10-7201-90 | 100µmoles | .051grams | 1.5 | .067 | 25.2 | 15.75 | 11.45 | ||
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
2",3"-ddG-CE Phosphoramidite
2",3"-ddG-CE Phosphoramidite
Glen Research
To avoid polymerase extension at the 3’ terminus, 2’,3’-dideoxynucleoside and 3’-deoxynucleoside CPGs have proved to be effective. 2’,3’-phosphoramidites are designed to be used with the 5’-phosphoramidites and supports. Since these phosphoramidites have no DMT group, they are not compatible with purification by the DMT-on technique. Ion exchange HPLC or PAGE should be used to purify these dideoxy terminated oligos to ensure that shorter sequences (containing 3’-OH) groups are removed. (3’-termination can also be effected using a 3’-3’ linkage formed using 5’-supports, or 3’-spacer C3 CPG.)
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures.
| ABI 392/394 | |||||||||
| Cat.No. | PackSize | Grams/Pack | 0.1M Dil.(mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 10-7201-02 | 0.25grams | .25grams | 4.94 | 151.33 | 90.8 | 56.75 | 41.27 | 30.27 | 7.57 |
| 10-7201-90 | 100µmoles | .051grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| Expedite | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 10-7201-02 | 0.25grams | .25grams | 7.37 | .07 | 141 | 88.13 | 64.09 | 8.81 | |
| 10-7201-90 | 100µmoles | .051grams | 1.5 | .07 | 23.6 | 14.75 | 10.73 | 1.48 | |
| Beckman | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 10-7201-02 | 0.25grams | .25grams | 7.37 | .07 | 142.6 | 89.13 | 64.82 | ||
| 10-7201-90 | 100µmoles | .051grams | 1.5 | .07 | 25.2 | 15.75 | 11.45 | ||
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
