Oligo Synthesis : CEPs
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2",3"-ddC-CE Phosphoramidite
2",3"-ddC-CE Phosphoramidite
Glen Research
| Catalogue No. | Description | Pack Size | Price | Qty |
|
|---|---|---|---|---|---|
| 10-7101-02 | 2",3"-ddC-CE Phosphoramidite | 0.25g | £436.00£414.20Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 10-7101-90 | 2",3"-ddC-CE Phosphoramidite | 100µmoles | £104.00£98.80Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
Related products
2",3"-ddC-CE Phosphoramidite
2",3"-ddC-CE Phosphoramidite
Glen Research
| 2",3"-ddC-CE Phosphoramidite |
Catalog Number: 10-7101-xx
Description: 2",3"-ddC-CE Phosphoramidite
| N4-diisobutylaminomethylidene-2",3"-dideoxyCytidine,5"-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite | ||
| Formula: C27H47N6O4P | M.W.: 550.68 | F.W.: 273.18 |
Diluent: Anhydrous AcetonitrileAdd fresh diluent to product vial to recommended concentration and swirl vial occasionally over several minutes until product is completely dissolved. (Some oils may require between 5 and 10 minutes.) Use care to maintain anhydrous conditions. In case of transfer to alternate vial type, ensure recipient vial has been pre-dried. For more information, see http://www.glenres.com/Technical/TB_ABITransfer.pdf. |
| Coupling: No changes needed from standard method recommended by synthesizer manufacturer. |
| Deprotection: Ammonium hydroxide at 55 °C for 17 hours. |
| Storage: Freezer storage, -10 to -30°C, dry |
| Stability in Solution: 2-3 days |
chain terminators
In situations where ligation must be blocked at the 5’ terminus, 5’-OMe-dT may be used. 5’-OMe modification of a strand of siRNA using 5’-OMe-T can control guide strand selection and targeting specificity.1 5’-Amino-dT terminates an oligonucleotide with a 5’-amino group which may be used for attaching a peptide or a PNA sequence. To avoid polymerase extension at the 3’ terminus, 2’,3’-dideoxynucleoside and 3’-deoxynucleoside CPGs have proved to be effective. 2’,3’- Phosphoramidites are designed to be used with the 5’-phosphoramidites and supports. Since these phosphoramidites have no DMT group, they are not compatible with purification by the DMT-on technique. Ion exchange HPLC or PAGE should be used to purify these dideoxy terminated oligos to ensure that shorter sequences (containing 3’-OH) groups are removed. (3’-Termination can also be effected using a 3’-3’ linkage formed using 5’-supports, or 3’-spacer C3 CPG.)
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
2",3"-ddC-CE Phosphoramidite
2",3"-ddC-CE Phosphoramidite
Glen Research
MSDS
Glen Report
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
2",3"-ddC-CE Phosphoramidite
2",3"-ddC-CE Phosphoramidite
Glen Research
To avoid polymerase extension at the 3’ terminus, 2’,3’-dideoxynucleoside and 3’-deoxynucleoside CPGs have proved to be effective. 2’,3’-phosphoramidites are designed to be used with the 5’-phosphoramidites and supports. Since these phosphoramidites have no DMT group, they are not compatible with purification by the DMT-on technique. Ion exchange HPLC or PAGE should be used to purify these dideoxy terminated oligos to ensure that shorter sequences (containing 3’-OH) groups are removed. (3’-termination can also be effected using a 3’-3’ linkage formed using 5’-supports, or 3’-spacer C3 CPG.)
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures.
| ABI 392/394 | |||||||||
| Cat.No. | PackSize | Grams/Pack | 0.1M Dil.(mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 10-7101-02 | 0.25grams | .25grams | 4.54 | 138 | 82.8 | 51.75 | 37.64 | 27.6 | 6.9 |
| 10-7101-90 | 100µmoles | .055grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| Expedite | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 10-7101-02 | 0.25grams | .25grams | 6.78 | .07 | 129.2 | 80.75 | 58.73 | 8.08 | |
| 10-7101-90 | 100µmoles | .055grams | 1.5 | .07 | 23.6 | 14.75 | 10.73 | 1.48 | |
| Beckman | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 10-7101-02 | 0.25grams | .25grams | 6.78 | .07 | 130.8 | 81.75 | 59.45 | ||
| 10-7101-90 | 100µmoles | .055grams | 1.5 | .07 | 25.2 | 15.75 | 11.45 | ||
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
