Oligo Synthesis : CEPs
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1-Me-dA-CE Phosphoramidite
1-Me-dA-CE Phosphoramidite
Glen Research
| Catalogue No. | Description | Pack Size | Price | Qty |
|
|---|---|---|---|---|---|
| 10-1501-02 | 1-Me-dA-CE Phosphoramidite | 0.25g | £600.00£570.00Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 10-1501-90 | 1-Me-dA-CE Phosphoramidite | 100µmoles | £200.00£190.00Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 10-1501-95 | 1-Me-dA-CE Phosphoramidite | 50µmoles | £100.00£95.00Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
Related products
1-Me-dA-CE Phosphoramidite
1-Me-dA-CE Phosphoramidite
Glen Research
| 1-Me-dA-CE Phosphoramidite |
Catalog Number: 10-1501-xx
Description: 1-Me-dA-CE Phosphoramidite
| 5"-Monomethoxytrityl-N6-chloroacetyl-1-methyl-2"-deoxyAdenosine,3"-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite | ||
| Formula: C42H49ClN7O6P | M.W.: 814.31 | F.W.: 328.24 |
Diluent: Anhydrous Acetonitrile |
| Coupling:No changes needed from standard method recommended by synthesizer manufacturer. Monomers that allow for UltraMILD deprotection are recommended.(dA:10-1601-xx, dC: 10-1015-xx, dG: 10-1621-xx, dT: 10-1030-xx ).To avoid any exchange of the iPr-Pac group on the dG with acetyl, use the UltraMild Cap Mix A (40-4210-xx/ 40-4212-xx).0.02M Iodine for oxidation isalso recommended. |
| Deprotection: 2 M Ammonia in methanol, 24 hours at Room Temperature using UltraMILD amidites or 60 hours at Room Temperature if using standard amidites. |
| Storage: Refrigerated storage, maximum of 2-8°C, dry |
| Stability in Solution: Similar to dA,C,G,T-CE Phosphoramidites |
MUTAGENESIS
Cellular polynucleotides are alkylated by endogenous components, such asS-adenosylmethionine, or after reacting with two general classes ofenvironmental and laboratory chemicals. SN1 chemical agents includealkylnitrosourea and N-alkyl-N-nitro-N-nitrosoguanidine that react with the N7position of guanine, N3 of adenine, O6 of guanine, O2 or O4 of pyrimidines, andthe non-phosphodiester oxygen atoms of the phosphate backbone. In contrast, SN2chemical agents such as methyl methanesulfonate and dimethyl sulfate reactprimarily with the N1 position of adenine (1-Methyl-2’-deoxyadenosine) and N3 ofcytosine. To avoid chain branching during synthesis when using DCI as activator,N6-Me-dA is offered with acetyl protection.
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
1-Me-dA-CE Phosphoramidite
1-Me-dA-CE Phosphoramidite
Glen Research
MSDS
Glen Report
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
1-Me-dA-CE Phosphoramidite
1-Me-dA-CE Phosphoramidite
Glen Research
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures.
| ABI 392/394 | |||||||||
| Cat.No. | PackSize | Grams/Pack | 0.1M Dil.(mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 10-1501-90 | 100µmoles | .081grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| 10-1501-02 | 0.25grams | .25grams | 3.07 | 89 | 53.4 | 33.38 | 24.27 | 17.8 | 4.45 |
| 10-1501-95 | 50µmoles | .041grams | .5 | 3.33 | 2 | 1.25 | .91 | .67 | .17 |
| Expedite | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 10-1501-90 | 100µmoles | .081grams | 1.5 | .07 | 23.6 | 14.75 | 10.73 | 1.48 | |
| 10-1501-02 | 0.25grams | .25grams | 4.58 | .07 | 85.2 | 53.25 | 38.73 | 5.33 | |
| 10-1501-95 | 50µmoles | .041grams | .75 | .07 | 8.6 | 5.38 | 3.91 | .54 | |
| Beckman | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 10-1501-90 | 100µmoles | .081grams | 1.5 | .07 | 25.2 | 15.75 | 11.45 | ||
| 10-1501-02 | 0.25grams | .25grams | 4.58 | .07 | 86.8 | 54.25 | 39.45 | ||
| 10-1501-95 | 50µmoles | .041grams | .75 | .07 | 10.2 | 6.38 | 4.64 | ||
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
