Oligo Synthesis : CEPs
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1-Me-A-CE Phosphoramidite
1-Me-A-CE Phosphoramidite
Glen Research
| Catalogue No. | Description | Pack Size | Price | Qty |
|
|---|---|---|---|---|---|
| 10-3501-02 | 1-Me-A-CE Phosphoramidite | 0.25g | £800.00£741.00Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 10-3501-90 | 1-Me-A-CE Phosphoramidite | 100µmoles | £320.00£288.80Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 10-3501-95 | 1-Me-A-CE Phosphoramidite | 50µmoles | £168.00£144.40Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
1-Me-A-CE Phosphoramidite
1-Me-A-CE Phosphoramidite
Glen Research
| 1-Me-A-CE Phosphoramidite |
Catalog Number: 10-3501-xx
Description: 1-Me-A-CE Phosphoramidite
| 5"-Monomethoxytrityl-N6-chloroacetyl-1-methyl-Adenosine, 2"-O-TBDMS-3"-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite | ||
| Formula: C48H63ClN7O7PSi | M.W.: 944.57 | F.W.: 344.24 |
Diluent: Anhydrous Acetonitrile |
| Coupling: 15 minute coupling time recommended.Monomers that allow for UltraMILD deprotection are recommended.(Pac-A:10-3000-xx, Ac-C: 10-3015-xx, iPr-Pac-G: 10-3021-xx, U: 10-3030-xx ). |
| Deprotection: 2 M Ammonia in methanol, 24 hours at Room Temperature using UltraMILD amidites or 60 hours at Room Temperature if using amidites with standard protecting groups. |
| Storage: Refrigerated storage, maximum of 2-8°C, dry |
| Stability in Solution: Similar to dA,C,G,T-CE Phosphoramidites |
Methylation of adenosine at position 1 produces a drasticfunctional change in the nucleobase. 1-Methyladenosine (pKa 8.25) is a muchstronger base than adenosine (pKa 3.5). N-1 methylation excludes participationof the adenine base in canonical Watson–Crick base pairing and provides apositive charge to the nucleobase. This modification also alters thehydrophobicity of the base, the stacking properties, the ordering of watermolecules and the chelation properties. The base may become involved innon-canonical hydrogen bonding, in electrostatic interactions and, in general,it may contribute to the conformational dynamics of the tRNA.
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1-Me-A-CE Phosphoramidite
1-Me-A-CE Phosphoramidite
Glen Research
MSDS
Glen report
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
1-Me-A-CE Phosphoramidite
1-Me-A-CE Phosphoramidite
Glen Research
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.
| ABI 392/394 | |||||||||
| Cat.No. | PackSize | Grams/Pack | 0.1M Dil.(mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 10-3501-95 | 50µmoles | .047grams | .5 | 3.33 | 2 | 1.25 | .91 | .67 | .17 |
| 10-3501-90 | 100µmoles | .094grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| 10-3501-02 | 0.25grams | .25grams | 2.65 | 75 | 45 | 28.13 | 20.45 | 15 | 3.75 |
| Expedite | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 10-3501-95 | 50µmoles | .047grams | .75 | .07 | 8.6 | 5.38 | 3.91 | .54 | |
| 10-3501-90 | 100µmoles | .094grams | 1.5 | .07 | 23.6 | 14.75 | 10.73 | 1.48 | |
| 10-3501-02 | 0.25grams | .25grams | 3.95 | .07 | 72.6 | 45.38 | 33 | 4.54 | |
| Beckman | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 10-3501-95 | 50µmoles | .047grams | .75 | .07 | 10.2 | 6.38 | 4.64 | ||
| 10-3501-90 | 100µmoles | .094grams | 1.5 | .07 | 25.2 | 15.75 | 11.45 | ||
| 10-3501-02 | 0.25grams | .25grams | 3.95 | .07 | 74.2 | 46.38 | 33.73 | ||
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
1-Me-A-CE Phosphoramidite
1-Me-A-CE Phosphoramidite
Glen Research
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutionsand approximate couplings based on normal priming procedures.
| ABI 392/394 | |||||||||
| Cat.No. | PackSize | Grams/Pack | 0.1M Dil.(mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 10-3501-95 | 50µmoles | .047grams | .5 | 3.33 | 2 | 1.25 | .91 | .67 | .17 |
| 10-3501-90 | 100µmoles | .094grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| 10-3501-02 | 0.25grams | .25grams | 2.65 | 75 | 45 | 28.13 | 20.45 | 15 | 3.75 |
| Expedite | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 10-3501-95 | 50µmoles | .047grams | .75 | .07 | 8.6 | 5.38 | 3.91 | .54 | |
| 10-3501-90 | 100µmoles | .094grams | 1.5 | .07 | 23.6 | 14.75 | 10.73 | 1.48 | |
| 10-3501-02 | 0.25grams | .25grams | 3.95 | .07 | 72.6 | 45.38 | 33 | 4.54 | |
| Beckman | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 10-3501-95 | 50µmoles | .047grams | .75 | .07 | 10.2 | 6.38 | 4.64 | ||
| 10-3501-90 | 100µmoles | .094grams | 1.5 | .07 | 25.2 | 15.75 | 11.45 | ||
| 10-3501-02 | 0.25grams | .25grams | 3.95 | .07 | 74.2 | 46.38 | 33.73 | ||
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
