Lab Reagents: Smart Buffers and Reagents
Biological buffers for the life science laboratory need to meet a wide array of criteria. These include good stability, lack of toxicity, precision in pH, pKa between 6.0 and 8.0 (the region in which most biological reactions occur), minimal salt effects due to the ionic composition of the solution, free of enzymatic and hydrolytic activity and minimal participation in biological reactions. Buffers for molecular biology applications, e.g. Tris-Borate-EDTA and Tris-Acetate-EDTA, need to be free from DNAse and RNAse activity. Medicago"s SmartBuffers meet all these criteria. They are manufactured using highly purified, analytical grade chemicals and are extensively quality tested. Manufacturing is done in a controlled clean environment according to GMP procedures. A high level of automation essentially avoids operator intervention and contact with the products. SmartBuffers are supplied as exactly pre-weighed tablets packed in containers or blister packs or as exactly pre-weighed powder mixes packed in sealed aluminium foil pouches. •Pre-mixed powder buffers or tablets with pre-set pH•Analytical grade reagents •Eliminates calculation, formulation and weighing errors •Dissolve-and-go for greater convenience •Stable at room temperature for at least 3 years •Guaranteed reproducibility
SSPE (PBS-EDTA) Buffer pH 7.4
Medicago’s SSPE buffer is specially formulated for hybridization in Northern and Southern blotting and for wash steps in microarray techniques. The buffer can be used in various applications within molecular biology such as immunocyto-chemistry and cell culture procedures
Medicago AB
| Catalogue No. | Description | Pack Size | Price | Qty |
|
|---|---|---|---|---|---|
| MC-09-8952-100 | SSPE (PBS-EDTA) pH 7,.4 | 100 tablets | £53.00 | Quantity | Add to Order |
SSPE (PBS-EDTA) Buffer pH 7.4
Medicago’s SSPE buffer is specially formulated for hybridization in Northern and Southern blotting and for wash steps in microarray techniques. The buffer can be used in various applications within molecular biology such as immunocyto-chemistry and cell culture procedures
Medicago AB
Product description
Medicago’s SSPE buffer is specially formulated for hybridization in Northern and Southern blotting and for wash steps in microarray techniques. The buffer can be used in various applications within molecular biology such as immunocyto-chemistry and cell culture procedures. It is suitable as wash buffer for some cell cultures and for resuspending cells for FACS (fluorescence-activated cell sorter) techniques, in order to attain good cell sorting.
SSPE buffer tablets are exactly pre-weighed and supplied in a pack size of 100 tablets, each tablet giving 200 ml of 0.15 M sodium chloride, 0.001 M EDTA, 0.010 M sodium phosphate buffer with pH 7.4 at 25°C when dissovled in deionized or nulease free water.
Features
- Formulated from analytical grade chemicals
- Exactly pre-weighed tablets
- Time-saving
- Dissolve and go for greater convenience
- Ideal for standardizing laboratory work
Directions for use
Deposit one tablet in a laboratory flask or beaker placed on a magnetic stirrer. Add 100 ml of deionized water and stir the solution for a few minutes. Adjust the volume up to 200 ml, stir until full dissolution and the buffer is ready to use.
Shipping and storage
SSPE buffer is shipped at room temperature. Store the tablets in a dry place at room temeprature. Shelf life is three years after production date.
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
SSPE (PBS-EDTA) Buffer pH 7.4
Medicago’s SSPE buffer is specially formulated for hybridization in Northern and Southern blotting and for wash steps in microarray techniques. The buffer can be used in various applications within molecular biology such as immunocyto-chemistry and cell culture procedures
Medicago AB
Specifications
Chemicals: Analytical grade
Format: Exactly pre-weighed tablets
Composition: 0.15 M NaCl,0.001 M EDTA, 0.010 M Na PO43-
Volume: 200 ml
pH: 7.4 ± 0.05 at 25°
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
SSPE (PBS-EDTA) Buffer pH 7.4
Medicago’s SSPE buffer is specially formulated for hybridization in Northern and Southern blotting and for wash steps in microarray techniques. The buffer can be used in various applications within molecular biology such as immunocyto-chemistry and cell culture procedures
Medicago AB
Applications
- Nucleic acid hybridization and probe pre-hybrdization
- Wash buffer for microarrays
- Immunocytochemistry
- Cell culture procedures
- FACS
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
