Lab Reagents: Smart Buffers and Reagents
Biological buffers for the life science laboratory need to meet a wide array of criteria. These include good stability, lack of toxicity, precision in pH, pKa between 6.0 and 8.0 (the region in which most biological reactions occur), minimal salt effects due to the ionic composition of the solution, free of enzymatic and hydrolytic activity and minimal participation in biological reactions. Buffers for molecular biology applications, e.g. Tris-Borate-EDTA and Tris-Acetate-EDTA, need to be free from DNAse and RNAse activity. Medicago"s SmartBuffers meet all these criteria. They are manufactured using highly purified, analytical grade chemicals and are extensively quality tested. Manufacturing is done in a controlled clean environment according to GMP procedures. A high level of automation essentially avoids operator intervention and contact with the products. SmartBuffers are supplied as exactly pre-weighed tablets packed in containers or blister packs or as exactly pre-weighed powder mixes packed in sealed aluminium foil pouches. •Pre-mixed powder buffers or tablets with pre-set pH•Analytical grade reagents •Eliminates calculation, formulation and weighing errors •Dissolve-and-go for greater convenience •Stable at room temperature for at least 3 years •Guaranteed reproducibility
Sodium dodecyl sulphate (SDS)
The SDS reagent is an anionic detergent that denatures secondary and non-disulfide-linked tertiary protein structures. It applies a negative charge to each protein in proportion to its mass. SDS binds in a ratio of approximately 1.4 g SDS per 1.0 g protein, unfolding it to give a near-uniform negative charge along the length of the polypeptide chain.
Medicago AB
| Catalogue No. | Description | Pack Size | Price | Qty |
|
|---|---|---|---|---|---|
| MC-09-2026-1000 | Sodium dodecyl sulphate (SDS) 0.5 g | 1000 tablets | £218.00 | Quantity | Add to Order |
| MC-09-2026-50 | Sodium dodecyl sulphate (SDS) 0.5 g | 50 tablets | £23.00 | Quantity | Add to Order |
| MC-12-9193-5 | Sodium dodecyl sulphate (SDS) 10 %, 1000 ml | 5 pouches | £292.00 | Quantity | Add to Order |
| MC-12-9194-5 | Sodium dodecyl sulphate (SDS) 20 %, 1000 ml | 5 pouches | £502.00 | Quantity | Add to Order |
Sodium dodecyl sulphate (SDS)
The SDS reagent is an anionic detergent that denatures secondary and non-disulfide-linked tertiary protein structures. It applies a negative charge to each protein in proportion to its mass. SDS binds in a ratio of approximately 1.4 g SDS per 1.0 g protein, unfolding it to give a near-uniform negative charge along the length of the polypeptide chain.
Medicago AB
Product description
The SDS reagent is an anionic detergent that denatures secondary and non-disulfide-linked tertiary protein structures. It applies a negative charge to each protein in proportion to its mass. SDS binds in a ratio of approximately 1.4 g SDS per 1.0 g protein, unfolding it to give a near-uniform negative charge along the length of the polypeptide chain. As a result, the distance of migration through the gel in SDS-PAGE electrophoresis can be assumed to be directly related only to the size of the protein.
Features
- Prepared from analytical grade reagent
- Anionic detergent
- Denaturing agent used in SDS-PAGE
- Choice of tablets and powder in pouches
- Dissolve and use in minutes
Medicago’s SDS reagent is supplied in pre-weighed tablets. One tablet weighs 0.5 g and can be dissolved in deionized water to the desired volume and concentration. SDS reagent powder mix in pouches give 1000 ml of 10% or 20% sodium dodecyl sulphate solution.
Directions for use
Empty one pouch or deposit one tablet of the SDS reagent in a laboratory flask or beaker placed on a magnetic stirrer. Add 300 ml of deionized water and stir the solution for a few minutes. Adjust the volume up to 1000 ml, stir until full dissolution and the solution is ready to use.
Deposit one tablet of the SDS reagent in a laboratory flask or beaker placed on a magnetic stirrer. Add deionized water and stir the solution for a few minutes until full dissolution. Adjust the volume up to required concentration and the solution is ready to use.
Shipping and storage
The SDS reagent is shipped at room temperature. Store the tablets and pouches in a dry place at room temperature. Shelf life is three years after production date.
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
Sodium dodecyl sulphate (SDS)
The SDS reagent is an anionic detergent that denatures secondary and non-disulfide-linked tertiary protein structures. It applies a negative charge to each protein in proportion to its mass. SDS binds in a ratio of approximately 1.4 g SDS per 1.0 g protein, unfolding it to give a near-uniform negative charge along the length of the polypeptide chain.
Medicago AB
Specifications
Chemicals: Analytical grade
Format: Exactly pre-weighed tablets or powder
Concentration: 10% or 20% solution
Weight: 0.5 g/tablet
Volume: 1000 ml
pH: 3.0 ± 0.05 at 25°C
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
Sodium dodecyl sulphate (SDS)
The SDS reagent is an anionic detergent that denatures secondary and non-disulfide-linked tertiary protein structures. It applies a negative charge to each protein in proportion to its mass. SDS binds in a ratio of approximately 1.4 g SDS per 1.0 g protein, unfolding it to give a near-uniform negative charge along the length of the polypeptide chain.
Medicago AB
Applications
- Solubilizes and denatures proteins
- SDS-PAGE electrophoresis
- Western blots
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
