Lab Reagents: Smart Buffers and Reagents
Biological buffers for the life science laboratory need to meet a wide array of criteria. These include good stability, lack of toxicity, precision in pH, pKa between 6.0 and 8.0 (the region in which most biological reactions occur), minimal salt effects due to the ionic composition of the solution, free of enzymatic and hydrolytic activity and minimal participation in biological reactions. Buffers for molecular biology applications, e.g. Tris-Borate-EDTA and Tris-Acetate-EDTA, need to be free from DNAse and RNAse activity. Medicago"s SmartBuffers meet all these criteria. They are manufactured using highly purified, analytical grade chemicals and are extensively quality tested. Manufacturing is done in a controlled clean environment according to GMP procedures. A high level of automation essentially avoids operator intervention and contact with the products. SmartBuffers are supplied as exactly pre-weighed tablets packed in containers or blister packs or as exactly pre-weighed powder mixes packed in sealed aluminium foil pouches. •Pre-mixed powder buffers or tablets with pre-set pH•Analytical grade reagents •Eliminates calculation, formulation and weighing errors •Dissolve-and-go for greater convenience •Stable at room temperature for at least 3 years •Guaranteed reproducibility
IMAC Phosphate & Elution Buffer Tablets
Immobilized metal ion affinity chromatography (IMAC) is a widely used separation method for purifying proteins and peptides that have an affinity for metal ions, such as histidine-tagged proteins but also some untagged recombinant or native proteins. Medicago’s IMAC phosphate and elution buffer tablets have been developed exclusively for use in this method.
Medicago AB
| Catalogue No. | Description | Pack Size | Price | Qty |
|
|---|---|---|---|---|---|
| MC-09-1010-50 | IMAC phosphate buffer | 50 tablets | £64.00 | Quantity | Add to Order |
| MC-09-1012-10 | IMAC elution buffer | 10 tablets /blister pack | £37.00 | Quantity | Add to Order |
| MC-09-1012-50 | IMAC elution buffer | 50 tablets | £107.00 | Quantity | Add to Order |
IMAC Phosphate & Elution Buffer Tablets
Immobilized metal ion affinity chromatography (IMAC) is a widely used separation method for purifying proteins and peptides that have an affinity for metal ions, such as histidine-tagged proteins but also some untagged recombinant or native proteins. Medicago’s IMAC phosphate and elution buffer tablets have been developed exclusively for use in this method.
Medicago AB
Features
- Dissolve and go
- Exactly pre-weighed tablets in bottles
- Mix elution buffer with phosphate buffer to achieve desired imidazole concentration
- Ultrapure, low UV280 absorbance imidazole
- Formulated from analytical grade chemicals
Product description
Immobilized metal ion affinity chromatography (IMAC) is a widely used separation method for purifying proteins and peptides that have an affinity for metal ions, such as histidine-tagged proteins but also some untagged recombinant or native proteins. Medicago’s IMAC phosphate and elution buffer tablets have been developed exclusively for use in this method.
IMAC buffer tablets are supplied in bottles and in blister packs. One tablet of phosphate buffer dissolved in deionized water yields a 300 mM sodium chloride, 50 mM sodium phosphate buffer with pH 8.0 at 22°C. One tablet of elution buffer yields a 300 mM sodium chloride, 300 mM imidazole, 50 mM sodium phosphate buffer with pH 8.0 at 22°C.
Applications
- Purification of histidine-tagged and non-histidine-taggedrecombinant and native proteins using IMAC chromatography
- The phosphate buffer may be used for other types of affinity chromatography methods
Directions for use
Prepare the phosphate and the elution buffers separately. Deposit one tablet in a laboratory flask or beaker placed on a magnetic stirrer. Add deionized water up to the given volume for each tablet and stir until full dissolution. Mix the phosphate buffer and elution buffer to achieve desired imidazole concentration, follow the dilution series included in the product sheet.
Shipping and storage
IMAC phosphate and elution buffer tablets are shipped at room temperature. Store the tablets in a dry place at room temperature. Shelf life for IMAC phosphate buffer tablets is five years and shelf life for IMAC elution buffer tablets is three years.
Specifications
Chemicals: Analytical grade
Format: Exactly pre-weighed tablets
Composition:
Phosphate Buffer
300 mM Sodium Chloride
50 mM Sodium Phosphate
Elution Buffer
300 mM Sodium Chloride
300 mM Imidazole
50 mM Sodium Phosphate
Volume:
Phosphate Buffer: 400 ml Elution Buffer: 100 ml
pH 8.0 ± 0.05 at 22°C
Shelf life:
Phosphate Buffer: 5 years after production date Elution Buffer: 3 years after production date
MSDS:
MSDS IMAC Elution Buffer TabletsMSDS IMAC Phosphate Buffer TabletsIf you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
