Lab Reagents: Smart Buffers and Reagents
Biological buffers for the life science laboratory need to meet a wide array of criteria. These include good stability, lack of toxicity, precision in pH, pKa between 6.0 and 8.0 (the region in which most biological reactions occur), minimal salt effects due to the ionic composition of the solution, free of enzymatic and hydrolytic activity and minimal participation in biological reactions. Buffers for molecular biology applications, e.g. Tris-Borate-EDTA and Tris-Acetate-EDTA, need to be free from DNAse and RNAse activity. Medicago"s SmartBuffers meet all these criteria. They are manufactured using highly purified, analytical grade chemicals and are extensively quality tested. Manufacturing is done in a controlled clean environment according to GMP procedures. A high level of automation essentially avoids operator intervention and contact with the products. SmartBuffers are supplied as exactly pre-weighed tablets packed in containers or blister packs or as exactly pre-weighed powder mixes packed in sealed aluminium foil pouches. •Pre-mixed powder buffers or tablets with pre-set pH•Analytical grade reagents •Eliminates calculation, formulation and weighing errors •Dissolve-and-go for greater convenience •Stable at room temperature for at least 3 years •Guaranteed reproducibility
EDTA buffer pH 8.0
The EDTA (ethylene-diamine-tetraacetic acid) molecule is a chelating agent widely used in molecular biology to sequester divalent and trivalent metal ions such as calcium and magnesium.
Medicago AB
| Catalogue No. | Description | Pack Size | Price | Qty |
|
|---|---|---|---|---|---|
| MC-12-9160-5 | EDTA buffer pH 8.0, 1000 ml | 5 pouches | £188.00 | Quantity | Add to Order |
| MC-12-9161-5 | EDTA buffer pH 8.0, 500 ml | 5 pouches | £132.00 | Quantity | Add to Order |
EDTA buffer pH 8.0
The EDTA (ethylene-diamine-tetraacetic acid) molecule is a chelating agent widely used in molecular biology to sequester divalent and trivalent metal ions such as calcium and magnesium.
Medicago AB
Product description
The EDTA (ethylene-diamine-tetraacetic acid) molecule is a chelating agent widely used in molecular biology to sequester divalent and trivalent metal ions such as calcium and magnesium. This ability prevents DNA and RNA degradation as metal-dependent enzymes acting as nucleases becomes deactivated. A fully deprotonated EDTA molecule will bind directly to the metal ion making the buffer suitable for adding to stored blood as an anti-coagulant to bind Ca2 ions. Furthermore, EDTA is useful for cell culture procedures as it prevents clumping of cells in liquid suspension and detaches adherent cells when passaging.
Medicago’s EDTA buffer is supplied in two sizes with pouches giving 500 ml or 1000 ml of 0.50 M EDTA buffer with pH 8.0 at 25°C when the contents of one pouch is dissolved in deionized water.
Shipping and storage
EDTA buffer is shipped at room temperature. Store the pouches in a dry place at room temperature. Shelf life is three years after production date.
Specifications
Chemicals: Analytical grade
Format: Exactly pre-weighed powder mix
Concentration: 0.50 M EDTA
Volume: 500 ml and 1000 ml
pH: 8.0 ± 0.05 at 25°C
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
EDTA buffer pH 8.0
The EDTA (ethylene-diamine-tetraacetic acid) molecule is a chelating agent widely used in molecular biology to sequester divalent and trivalent metal ions such as calcium and magnesium.
Medicago AB
Directions for use
Empty one pouch of the EDTA buffer in a laboratory flask or beaker placed on a magnetic stirrer. Add 300 ml of deionized water and stir the solution for a few minutes. Adjust the volume up to 500 ml or 1000 ml, stir until full dissolution and the buffer solution is ready to use.
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
EDTA buffer pH 8.0
The EDTA (ethylene-diamine-tetraacetic acid) molecule is a chelating agent widely used in molecular biology to sequester divalent and trivalent metal ions such as calcium and magnesium.
Medicago AB
Features
- Chelating agent
- Prevents DNA/RNA degradation
- Pre-set pH
- Exactly pre-weighed in pouches
Applications
- Added to stored blood as an anticoagulant
- Inhibits metal-dependent enymatic reactions
- Prevents cell-to-cell joining of cadherins
- Used in electrophoresis buffers TAE and TBE
- Component in TE buffers for DNA and RNA applications
- Cell culture procedures
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
