Enzymes for Molecular Biology: Enzymes for Molecular Biology
T4 Polynucleotide Kinase, Cloned
T4 Polynucleotide Kinase (PNK) catalyses the transfer of the g-phosphate from ATP to the 5"-hydroxyl of single- or double-stranded DNA, RNA, and nucleoside 3"-monophosphates
BioSearch Tech (Lucigen/Epicentre)
| Catalogue No. | Description | Pack Size | Price | Qty |
|
|---|---|---|---|---|---|
| P0503K | T4 Polynucleotide Kinase, Cloned | 10U/µl 3,000U | £155.00 | Quantity | Add to Order |
Related products
T4 Polynucleotide Kinase, Cloned
T4 Polynucleotide Kinase (PNK) catalyses the transfer of the g-phosphate from ATP to the 5"-hydroxyl of single- or double-stranded DNA, RNA, and nucleoside 3"-monophosphates
BioSearch Tech (Lucigen/Epicentre)
T4 Polynucleotide Kinase (PNK) catalyses the transfer of the g-phosphate from ATP to the 5"-hydroxyl of single- or double-stranded DNA, RNA, and nucleoside 3"-monophosphates. The enzyme also removes the 3"-phosphate from 3"-phosphoryl polynucleotides, deoxyribonucleoside 3"-monophosphates, and deoxyribonucleoside 3", 5"-diphosphates to form a 3"-hydroxyl group.
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Unit Definition
Under standard conditions, one unit converts 1nmole of 32P from [g-32P]-ATP into an acid-insoluble form in 30 minutes at 37°C in 33 mM Tris-acetate, pH 7.8, 66 mM potassium acetate, 10mM magnesium acetate, 0.5mM DTT, 1.0mM ATP, and 0.1mg/ml micrococcal nuclease-treated calf thymus DNA as substrate.
Storage Buffer
50% glycerol containing 50mM Tris-HCl, pH 7.5, 0.1 M NaCl, 0.1mM EDTA, 0.1% Triton® X-100, and 1 mM DTT.
T4 PNK 10X Reaction Buffer
330mM Tris-acetate, pH 7.8, 660mM potassium acetate, 100mM magnesium acetate, and 5 mM DTT. A 10mM solution of ATP for non-isotopic applications is available separately.
Quality Control
T4 PNK is tested in 5"-phosphorylation of nucleic acids and is free of detectable nuclease activities.
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
T4 Polynucleotide Kinase, Cloned
T4 Polynucleotide Kinase (PNK) catalyses the transfer of the g-phosphate from ATP to the 5"-hydroxyl of single- or double-stranded DNA, RNA, and nucleoside 3"-monophosphates
BioSearch Tech (Lucigen/Epicentre)
Protocols for: T4 Polynucleotide Kinase, Cloned
T4 Polynucleotide Kinase Protocol
(catalogue number P0505H / P0501K / P0503K)
Please note: all protocols off site are the responsibility of the products supplier
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
T4 Polynucleotide Kinase, Cloned
T4 Polynucleotide Kinase (PNK) catalyses the transfer of the g-phosphate from ATP to the 5"-hydroxyl of single- or double-stranded DNA, RNA, and nucleoside 3"-monophosphates
BioSearch Tech (Lucigen/Epicentre)
References
- Sambrook, J. et al. (1989) in: Molecular Cloning: A Laboratory Manual (2nd ed.), Cold Spring Harbor Laboratory Press, New York.
- Chaconas, G. et al. (1980) Meth. Enzymol. 65, 75.
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
T4 Polynucleotide Kinase, Cloned
T4 Polynucleotide Kinase (PNK) catalyses the transfer of the g-phosphate from ATP to the 5"-hydroxyl of single- or double-stranded DNA, RNA, and nucleoside 3"-monophosphates
BioSearch Tech (Lucigen/Epicentre)
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If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
T4 Polynucleotide Kinase, Cloned
T4 Polynucleotide Kinase (PNK) catalyses the transfer of the g-phosphate from ATP to the 5"-hydroxyl of single- or double-stranded DNA, RNA, and nucleoside 3"-monophosphates
BioSearch Tech (Lucigen/Epicentre)
Applications
- Labeling of 5´ termini of DNA and RNA with 32P or 33P for DNA sequencing, blot-hybridization experiments, or transcript mapping using Mung Bean Nuclease, S1 nuclease, or other nucleases.1,2
- Phosphorylation of oligonucleotide linkers and other DNA or RNA molecules prior to ligation, or for use in ligation amplification reactions with Ampligase® Thermostable DNA Ligase.
- Preparation of labeled DNA or RNA molecular weight markers for gel electrophoresis and chromatography.
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
