Enzymes for Molecular Biology: Enzymes for Molecular Biology
RNase I, E. coli
RNase I E. coli degrades single-stranded RNA to nucleoside 3"-monophosphates via 2", 3" cyclic monophosphate intermediates by cleaving every phosphodiester bond
BioSearch Tech (Lucigen/Epicentre)
| Catalogue No. | Description | Pack Size | Price | Qty |
|
|---|---|---|---|---|---|
| N6901K | RNase I | 10 U/µl 1,000U | £86.00 | Quantity | Add to Order |
Related products
RNase I, E. coli
RNase I E. coli degrades single-stranded RNA to nucleoside 3"-monophosphates via 2", 3" cyclic monophosphate intermediates by cleaving every phosphodiester bond
BioSearch Tech (Lucigen/Epicentre)
RNase I degrades single-stranded RNA to nucleoside 3"-monophosphates via 2", 3" cyclic monophosphate intermediates by cleaving every phosphodiester bond. RNase I completely degrades RNA, unlike RNase A that cleaves only after cytosine and uridine. In addition, the enzyme is completely inactivated by heating at 70°C for 20 minutes, eliminating the requirement to remove the enzyme prior to many subsequent procedures.
 |
| Figure 1. Removal of RNA from plasmid DNA preparations. Plasmid DNA was prepared from 1.5 ml of an overnight culture, suspended in 50 µl TE buffer (10 mM Tris-HCl (pH 8), 1 mM EDTA), and treated with RNase A, RNase I, or no enzyme. Five µl of each reaction were resolved by agarose gel electrophoresis and visualized by staining with ethidium bromide. Lane 1, no RNase; Lane 2, 1.5 U of RNase I; Lane 3, 20 µg/ml RNase A; MW, 1-kb ladder. Arrow denotes small undigested RNA. |
Unit Definition
One unit degrades 100ng of E. coli ribosomal RNA per second into acid-soluble nucleotides at 37°C in 10mM Tris-HCl, pH 7.5, 100mM NaCl, and 1mM EDTA.
Dilution and Storage Buffers
50mM Tris-HCl, pH 7.5, 100mM NaCl, 0.1mM EDTA in 50% glycerol.
Quality Control
RNase I is free of detectable DNA exo- and endonuclease activities.
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
RNase I, E. coli
RNase I E. coli degrades single-stranded RNA to nucleoside 3"-monophosphates via 2", 3" cyclic monophosphate intermediates by cleaving every phosphodiester bond
BioSearch Tech (Lucigen/Epicentre)
Protocols for: RNase I, E. coli
RNase I Protocol
(catalogue number N6901K / N6905k)
Please note: all protocols off site are the responsibility of the products supplier
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
RNase I, E. coli
RNase I E. coli degrades single-stranded RNA to nucleoside 3"-monophosphates via 2", 3" cyclic monophosphate intermediates by cleaving every phosphodiester bond
BioSearch Tech (Lucigen/Epicentre)
References
- Shen, V. and Schlessinger, D. (1982) The Enzymes XV (Part B), 501.
- delCardayré, S.B. and Raines, R.T. (1995) Anal. Biochem. 225, 176.
- Johnson, M.G. (1996) EPICENTRE Forum 3 (4), 7.
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
RNase I, E. coli
RNase I E. coli degrades single-stranded RNA to nucleoside 3"-monophosphates via 2", 3" cyclic monophosphate intermediates by cleaving every phosphodiester bond
BioSearch Tech (Lucigen/Epicentre)
 | Get your Epicentre® Forum - hot off the press!Click here for direct link to Forum listings |
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
RNase I, E. coli
RNase I E. coli degrades single-stranded RNA to nucleoside 3"-monophosphates via 2", 3" cyclic monophosphate intermediates by cleaving every phosphodiester bond
BioSearch Tech (Lucigen/Epicentre)
Applications
- Removal of RNA from DNA preparations.1
- RNase protection assays to detect single-basepair mismatches in RNA:RNA and RNA:DNA hybrids.1
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
