Enzymes for Molecular Biology: Enzymes for Molecular Biology

The Cambio DNA Isopolymerase family

The Cambio DNA Isopolymerase family of isothermal polymerases was developed to address the stringent molecular needs of isothermal sequence-specific amplification, specifically those applications in molecular research and diagnostics. Engineered on the backbone of DNA Pol I polymerases with a high degree of strand displacement activity, our isopolymerases were designed using directed molecular evolution technology to create unique homologues with high salt tolerance, a more flexible temperature range, and an even greater strand displacement activity to meet the demands of evolving isothermal applications.

Cambio DNA Isopolymeraseis a DNA polymerase, active at ambient temperatures. It exhibits no 5’-3’or 3’-5’ exonuclease activity. Isothermal Polymerase has high processivity and strong strand-displacement activity and can be heat inactivated at temperatures above 50°C.

Cambio DNA Isopolymerase SSD (strong strand displacement) is a DNA polymerase with stronger strand displacement than Cambio DNA Isopolymerase. It also exhibits high salt tolerance.

Cambio DNA Isopolymerase BST (Thermostable) is an engineered heat-tolerant Bst polymerase (large fragment) with enhanced strand-displacement activity and salt tolerance than BST polymerase. Cambio DNA isopolymerase BSTis active from 25 to 65°C. It is lacking 5’-3’- and 3’-5’-exonuclease activity.

Figure: Strand Displacement Activity in the Presence of Increasing Concentrations of Monovalent Salt. Cambio DNA Isopolymerase SSD has considerably higher strand displacement activity than Bsu polymerase. At 100 mM and 200 mM salt, a 2-fold and 3-fold higher strand displacement activity is observed, respectively (left).Cambio DNA Isopolymerase BST shows better strand displacement than Bst wild type at 100 mM salt and above, 3-fold higher activity at 300 mM (right)