Chromosome Painting: Human
Star*FISH© Human Chromosome-Y Heterochromatic Probes Ready-to-Use Format
Cambio"s Whole chromosome- specific, pan-centromeric, centromic, Pan-telomeric, telomeric, arm and band specific paints
Cambio
| Catalogue No. | Description | Pack Size | Price | Qty |
|
|---|---|---|---|---|---|
| 1125-YB | Chromosome Y (Heterochromatic) Probe | Biotin10 tests | £305.00 | Quantity | Add to Order |
| 1125-YF | Chromosome Y (Heterochromatic) Probe | FITC10 tests | £327.00 | Quantity | Add to Order |
Star*FISH© Human Chromosome-Y Heterochromatic Probes Ready-to-Use Format
Cambio"s Whole chromosome- specific, pan-centromeric, centromic, Pan-telomeric, telomeric, arm and band specific paints
Cambio
Chromosome Y Heterochromatic Region Probe
To supplement Cambio"s Chromosome Y Euchromatic probe and the whole chromosome Y paints, which do not paint heterochromatin, Cambio also provides a Yqh-specific probe. Available with biotin, or FITC labelling, it can be used in conjunction with other Star*FISH© paints and probes, and other Star*FISH© detection kits and reagents.
Please note: Human Chromosome Y Euchromatic paints are no longer available (Dec "06)
Protocol Listings
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
Star*FISH© Human Chromosome-Y Heterochromatic Probes Ready-to-Use Format
Cambio"s Whole chromosome- specific, pan-centromeric, centromic, Pan-telomeric, telomeric, arm and band specific paints
Cambio
The FISH protocol is divided into two stages. Denaturation and Hybridisationare performed on day one. Washing and Detection are performed on day two.The metaphase spreads are normally prepared a day in advance. On day one, the DNA of the chromosomes and paints is denatured and the Hybridisation process (reannealing) takes place overnight.
On day two the slides are washed to remove unbound DNA sequences followed by detection, counterstaining and mounting.
Day One - Denaturation & Hybridisation
/library/images/html_images/2019 protocols/Formatted Protocol G2.pdf
Day Two - Detection
Biotin Labelled Paints:
Biotin Labelled Chromosome detected with Cyanine 3*
Biotin Labelled Chromosome detected with Cyanine 5*
Biotin Labelled Chromosome detected with Texas Red (1082-KT-50)
Biotin Labelled Chromosome detected with FITC (1089-KB-50)
*Cyanine 3 and Cyanine 5 Streptavidin is no longer supplied by Cambio
FITC Labelled Paints:
FITC Amplification (1084-KF-50)
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
Star*FISH© Human Chromosome-Y Heterochromatic Probes Ready-to-Use Format
Cambio"s Whole chromosome- specific, pan-centromeric, centromic, Pan-telomeric, telomeric, arm and band specific paints
Cambio
Detection will be required for Biotin Labelled paints
FITC Labelled paints may require amplification depending on user experience and samples.
Please note: Probes,detection kits and amplification kits are sold separately
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
