Transfection - EZ Biosystems: Cell Lines
Avalanche®-Cell Type / Cell Line-specific Transfection Reagent series:
Perform maximum transfection efficiency for more than 170 different primary cells and cell lines including 124 cancer cell lines (e.g. All of NCI60 cancer cell lines).Each of the cell type/cell line-specific transfection reagents in our Avalanche® series was individually formulated.
For primary cells click HERE
To learn why you should use Avalanche®Cell Type-Specific Transfection reagents, clickHERE; To learn how EZ Biosystems developed Avalanche®Cell Type-Specific Transection Reagents, clickHERE
Malme-3M Cell Avalanche® Transfection Reagent (human melanoma cell)
Wait!! Before you use this reagent, maybe you should first try Avalanche®-Omni Transfection Reagent or Avalanche®-Everyday Transfection Reagent which works great on more than 90% of cell types tested.
EZ Biosystems
| Catalogue No. | Description | Pack Size | Price | Qty |
|
|---|---|---|---|---|---|
| EZT-MALM-1-05 | Malme-3M Cell Transfection Reagent (human melanoma cell) | 0.5ml | £399.00 | Quantity | Add to Order |
| EZT-MALM-1-15 | Malme-3M Cell Transfection Reagent (human melanoma cell) | 1.5ml | £699.00 | Quantity | Add to Order |
Malme-3M Cell Avalanche® Transfection Reagent (human melanoma cell)
Wait!! Before you use this reagent, maybe you should first try Avalanche®-Omni Transfection Reagent or Avalanche®-Everyday Transfection Reagent which works great on more than 90% of cell types tested.
EZ Biosystems
Cell Line Information: Cell Line Name: Malme-3M (ATCC® HTB-64™) Organism: Homo sapiens, human Tissue Derived from metastatic site: lung Cell Type: fibroblast Morphology: mixed Culture Properties: mixed adherent-suspension Biosafety Level; 1 Disease: Malignant melanoma Age: 43 years Gender: male Ethnicity: Caucasian Applications: This melanoma cell line was isolated from the same patient as Malme-3 (ATCC HTB-102), a normal skin fibroblast. The two cell lines provide tumor and normal counterparts for comparative in vitro melanoma studies. This is one of an extensive series of human tumor lines isolated and characterized by J. Fogh. Markers: HLA A2, Aw30, B13, B40( /-), DRw7 Tumorigenic: Yes Malme-3M is one of the cell lines of the NCI-60 panel which represents different cancer types and has been widely utilized for drug screening and molecular target identification.The Transfection Reagent: Transfection of suspension cells, or suspension/adherent mixed cells, such as Malme-3M cell, has always been challenging, both technically and financially. The most common systems currently used for molecular delivery to these cells include expensive electroporation instruments and relatively toxic chemical formulations.Malme-3M Cell Avalanche® Transfection Reagent is a new class of unique chemical formulations specifically formulated and optimized for transfecting Malme-3M cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for Malme-3M cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche® Cell type/cell line specific transfection reagent series?
Features:
- Unique formulation-maximize transfection performance in Malme-3M cells
- Extremely gentle to cells
- Deliver single or multiple plasmids
- Free of animal-derived components
- Simplest and straightforward protocols to Increase experimental throughput
- Active in serum-containing medium
- Compatible with transfection in any plate format
- Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.
- Great pricing
- Developed and manufactured by EZ Biosystems
| Data: | |||
| |||
| For Other Cells: | |||
| Malme-3M Cell Avalanche® Transfection Reagent (human melanoma cell) can also be used on the following cells with high transfection efficiencies. LOX-IMVI CellM14 Cell SK-MEL-2 Cell SK-MEL-5 CellUACC-257 CellUACC-62 CellVero Cell 293 Cell 293T/17 Cell Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list. |
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
