Transfection - EZ Biosystems: Cell Lines
Avalanche®-Cell Type / Cell Line-specific Transfection Reagent series:
Perform maximum transfection efficiency for more than 170 different primary cells and cell lines including 124 cancer cell lines (e.g. All of NCI60 cancer cell lines).Each of the cell type/cell line-specific transfection reagents in our Avalanche® series was individually formulated.
For primary cells click HERE
To learn why you should use Avalanche®Cell Type-Specific Transfection reagents, clickHERE; To learn how EZ Biosystems developed Avalanche®Cell Type-Specific Transection Reagents, clickHERE
LS 174T Cell Avalanche™ Transfection Reagent (human colon adenocarcinoma cell)
LS 174T Cell Avalanche™ Transfection Reagent is a new, proprietary solution specifically designed for transfection on LS 174T Cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for LS 174T Cells. No other transfection reagents can match the efficiency, convenience, and gentleness of LS 174T Cell Avalanche™ Transfection Reagent for transfection on LS 174T Cells.
EZ Biosystems
| Catalogue No. | Description | Pack Size | Price | Qty |
|
|---|---|---|---|---|---|
| EZT-LS17-1-05 | LS 174T Cell Transfection Reagent (human colon adenocarcinoma cell) | 0.5ml | £399.00 | Quantity | Add to Order |
| EZT-LS17-1-15 | LS 174T Cell Transfection Reagent (human colon adenocarcinoma cell) | 1.5ml | £699.00 | Quantity | Add to Order |
LS 174T Cell Avalanche™ Transfection Reagent (human colon adenocarcinoma cell)
LS 174T Cell Avalanche™ Transfection Reagent is a new, proprietary solution specifically designed for transfection on LS 174T Cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for LS 174T Cells. No other transfection reagents can match the efficiency, convenience, and gentleness of LS 174T Cell Avalanche™ Transfection Reagent for transfection on LS 174T Cells.
EZ Biosystems
Cell line Information:
Designations: LS 174T Depositors: Northwestern University Growth Properties: adherent Organism: Homo sapiens Morphology: epithelial Source:Organ: colon Tumor Stage: Dukes" type B Disease: colorectal adenocarcinoma Cellular Products: carcinoembryonic antigen (CEA), interleukin 10 (IL-10), interleukin 6 (IL-6), mucin Applications: Electron microscopic studies revealed abundant microvilli and intracytoplasmic mucin vacuoles Tumorigenic: Yes Age: 58 years Gender:female Ethnicity: Caucasian Comments: LS 174T is a variant of LS 180 (ATCC CL-187) that has been maintained by using trypsin in the subculture protocol. It is more easily subcultivated than that parent line. Electron microscopic studies revealed abundant microvilli and intracytoplasmic mucin vacuoles. LS 174T cells stain positively for cytokeratins. The line is positive for expression of c-myc, N-myc, H-ras, N-ras, Myb, and fos oncogenes.
The Transfection Reagent:
LS 174T Cell Avalanche™ Transfection Reagent is a new, proprietary solution specifically designed for transfection on LS 174T Cells. The proprietary formulation of lipids and polymersensures the highest possible transfection efficiencies and viabilities for LS 174T Cells. No other transfection reagents can match the efficiency, convenience, and gentleness of LS 174T Cell Avalanche™ Transfection Reagent for transfection on LS 174T Cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?
Features:
- Specifically optimized to deliver nucleic acids into LS 174T Cells
- Highest efficiency to ensure experimental success
- Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
- Synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cells lines
- Simplest and straightforward protocols
- Compatible with transfection in any plate formats
- Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.
- Developed and manufactured by EZ Biosystems
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| LS 174T Cell Avalanche™ Transfection Reagent (human colon adenocarcinoma cell) can also be used on the following cells with high transfection efficiencies. T84 Cell HCT 116 Cell DLD-1 Cell HT-29 Cell Caco-2 Cell LoVo Cell SW480 Cell SW620 Cell 293 Cell Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list. |
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