Transfection - EZ Biosystems: Cell Lines
Avalanche®-Cell Type / Cell Line-specific Transfection Reagent series:
Perform maximum transfection efficiency for more than 170 different primary cells and cell lines including 124 cancer cell lines (e.g. All of NCI60 cancer cell lines).Each of the cell type/cell line-specific transfection reagents in our Avalanche® series was individually formulated.
For primary cells click HERE
To learn why you should use Avalanche®Cell Type-Specific Transfection reagents, clickHERE; To learn how EZ Biosystems developed Avalanche®Cell Type-Specific Transection Reagents, clickHERE
KG-1 Cell Avalanche™ Transfection Reagent (human leukemia cell)
Transfection of suspension cells, such as KG-1 Cells, is a problem in the laboratory routine and further in tissue engineering and gene therapy. Most methods working effectively for adherent cells in culture fail to transfect suspension cells.KG-1 Cell Avalanche™ Transfection Reagent is a new class of unique chemical formulations specifically formulated and optimized for transfecting KG-1 Cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for KG-1 Cells
EZ Biosystems
| Catalogue No. | Description | Pack Size | Price | Qty |
|
|---|---|---|---|---|---|
| EZT-KG10-1-05 | KG-1 Cell Transfection Reagent (human leukemia cell) | 0.5 ml | £399.00 | Quantity | Add to Order |
| EZT-KG10-1-15 | KG-1 Cell Transfection Reagent (human leukemia cell) | 1.5 ml | £699.00 | Quantity | Add to Order |
KG-1 Cell Avalanche™ Transfection Reagent (human leukemia cell)
Transfection of suspension cells, such as KG-1 Cells, is a problem in the laboratory routine and further in tissue engineering and gene therapy. Most methods working effectively for adherent cells in culture fail to transfect suspension cells.KG-1 Cell Avalanche™ Transfection Reagent is a new class of unique chemical formulations specifically formulated and optimized for transfecting KG-1 Cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for KG-1 Cells
EZ Biosystems
Cell Line Information:
Designations: KG-1 Morphology: myeloblast Growth properties: Suspension Cellular products: HLA DR Reverse transcriptase: negative Antigen Expression: HLA A30, A31, B35, Cw4 Isoenzyme: AK-1, 0; ES-D, 1; G6PD, B; GLO-I, 2; Me-2, 1; PGM1, 1-2; PGM3, 0 Depositors: D.W. Golde The KG-1 cell line was derived by H.P. Koeffler and D.W. Golde A bone marrow aspirate was obtained from a 59-year-old Caucasian male with erythroleukemia that evolved into acute myelogenous leukemia. KG-1 cells spontaneously differentiate to granulocyte and macrophage like cells. They show a good response to colony stimulating factor(CSF). The line is EBNA negative (EBNA-). Cytogenetic analysis: The stemline chromosome
The Transfection Reagent:
Transfection of suspension cells, such as KG-1 Cells, is a problem in the laboratory routine and further in tissue engineering and gene therapy. Most methods working effectively for adherent cells in culture fail to transfect suspension cells.
KG-1 Cell Avalanche™ Transfection Reagent is a new class of unique chemical formulations specifically formulated and optimized for transfecting KG-1 Cells. The proprietary formulation of lipids and polymersensures the highest possible transfection efficiencies and viabilities for KG-1 Cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?
Features:
- Specifically optimized to deliver nucleic acids into KG-1 Cells
- Highest efficiency to ensure experimental success
- Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
- Synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cells lines
- Simplest and straightforward protocols
- Suitable for Reverse Transfection
- Economical: High efficiency means less amount of nucleic acid & reagent is needed
- Developed and manufactured by EZ Biosystems
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| KG-1 Cell Avalanche™ Transfection Reagent (human leukemia cell) can also be used on the following cells with high transfection efficiencies. Caki-1 Cell 293 Cell 293T/17 Cell NIH/3T3 Cell 3T3-L1 Cell CT26.WT Cell NCTC Clone 929 Cell Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list. |
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