Transfection - EZ Biosystems: Cell Lines

Cell Line Information: Designations: IMR-32 Organism: Homo sapiens (human) Tissue: neuroblastoma; brain; derived from metastatic site: abdominal mass Age: 13 months Gender: male Ethnicity: Caucasian Morphology: neuroblast and fibroblast Growth properties: adherent Doubling time: approximately 20 hours VirusSuscept: vesicular stomatitis (Indiana); herpes simplex; vaccinia; coxsackievirus B3; poliovirus 3 (poorly) VirusResist: echovirus 11 Depositors: W.W. Nichols The IMR-32 cell line was established by W.W. Nichols, J. Lee and S. Dwight in April, 1967 from an abdominal mass occurring in a 13-month-old Caucasian male. The tumor was diagnosed as a neuroblastoma with rare areas of organoid differentiation. Two cell types are present. Predominant is a small neuroblast-like cell. The other is a large hyaline fibroblast.

The Transfection Reagent:

IMR-32 Cell Avalanche™ Transfection Reagent is a new class of unique chemical formulations specifically formulated and optimized for transfecting IMR-32 Cells. The proprietary formulation of lipids and polymersensures the highest possible transfection efficiencies and viabilities for IMR-32 Cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?

Features:

• Specifically optimized to deliver nucleic acids into IMR-32 Cells
• Highest efficiency to ensure experimental success
• Extremely gentle to cells
• Deliver single or multiple plasmids
• Synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cells lines
• Simplest and straightforward protocols
• Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.
• Developed and manufactured by EZ Biosystems

Data:

FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on IMR-32 cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for IMR-32 cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this IMR-32 Cell Avalanche Transfection Reagent.

For Other Cells:

IMR-32 Cell Avalanche™ Transfection Reagent (human neuroblastoma cell) can also be used on the following cells with high transfection efficiencies. LA-N-2 SK-N-SH Detroit 551 Cell MG-63 Cell WI-38 Cell MRC-5 Cell IMR-90 Cell COS-7 Cell Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.

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