Transfection - EZ Biosystems: Cell Lines

Cell Line Information: Cell Line Name: HOP-92, HOP92 Patient"s Age: 62 Patient"s Sex: M Prior Treatment: No Tissue Type: Lung, Carcinoma; Large cell carcinoma Histology: Large cell-ud; epithelium p53 Status: 0 ECAD Methylation: 22 MDR Function: -4 Institution: Johns Hopkins University School of Medicine Contributor: Liu, M HOP-92 is one of the cell lines of the NCI-60 panel which represents different cancer types and has been widely utilized for drug screening and molecular target identification.The Transfection Reagent: HOP-92 Cell Avalanche® Transfection Reagent is a new class of unique chemical formulations specifically formulated and optimized for transfecting HOP-92 cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for HOP-92 cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche® Cell type/cell line specific transfection reagent series?

Features:

• Specifically optimized to deliver nucleic acids into HOP-92 cells
• Highest efficiency to ensure experimental success
• Extremely gentle to cells
• Synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cells lines
• Easy-to-use protocol: Just mix nucleic acids with the reagent to form complexes and simply add the mixture to cells
• Active in serum-containing medium
• Suitable for Reverse Transfection
• Compatible with transfection in any plate format
• Economical: High efficiency means less amount of nucleic acid & reagent is needed
• Developed and manufactured by EZ Biosystems

Data:

FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on HOP-92 cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for HOP-92 cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this HOP-92 Cell Avalanche® Transfection Reagent.

For Other Cells:

HOP92 Cell Avalanche® Transfection Reagent (human lung carcinoma cell) can also be used on the following cells with high transfection efficiencies. EKVX CellHOP-62 Cell NCI-H226 CellNCI-H322M Cell NCI-H460 Cell Calu-3 Cell NCI-H358 Cell 293 Cell Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.

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