Transfection - EZ Biosystems: Cell Lines
Avalanche®-Cell Type / Cell Line-specific Transfection Reagent series:
Perform maximum transfection efficiency for more than 170 different primary cells and cell lines including 124 cancer cell lines (e.g. All of NCI60 cancer cell lines).Each of the cell type/cell line-specific transfection reagents in our Avalanche® series was individually formulated.
For primary cells click HERE
To learn why you should use Avalanche®Cell Type-Specific Transfection reagents, clickHERE; To learn how EZ Biosystems developed Avalanche®Cell Type-Specific Transection Reagents, clickHERE
EL4 Cell Avalanche™ Transfection Reagent (mouse lymphoma cell)
Transfection of suspension cells, such as EL4 Cells, is a problem in the laboratory routine and further in tissue engineering and gene therapy. Most methods working effectively for adherent cells in culture fail to transfect suspension cells.EL4 Cell Avalanche™ Transfection Reagent is a new class of unique chemical formulations specifically formulated and optimized for transfecting EL4 Cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for EL4 Cells.
EZ Biosystems
| Catalogue No. | Description | Pack Size | Price | Qty |
|
|---|---|---|---|---|---|
| EZT-EL40-1-05 | EL4 Cell Transfection Reagent (mouse lymphoma cell) | 0.5ml | £399.00 | Quantity | Add to Order |
| EZT-EL40-1-15 | EL4 Cell Transfection Reagent (mouse lymphoma cell) | 1.5ml | £699.00 | Quantity | Add to Order |
EL4 Cell Avalanche™ Transfection Reagent (mouse lymphoma cell)
Transfection of suspension cells, such as EL4 Cells, is a problem in the laboratory routine and further in tissue engineering and gene therapy. Most methods working effectively for adherent cells in culture fail to transfect suspension cells.EL4 Cell Avalanche™ Transfection Reagent is a new class of unique chemical formulations specifically formulated and optimized for transfecting EL4 Cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for EL4 Cells.
EZ Biosystems
Cell Line Information:
Designations: EL4 Depositors: M Cohn Growth Properties: suspension Organism: Mus musculus Morphology: lymphoblast Disease: lymphoma Strain: C57BL/6N Cell Type: T lymphocyte; Applications: transfection host Antigen Expression: H-2b; Thy-1.2 EL4 was established from a lymphoma induced in a C57BL mouse by 9,10-dimethyl-1,2-benzanthracene. The cells are resistant to 0.1 mM cortisol and sensitive to 20 mcg/ml PHA.
The Transfection Reagent:
Transfection of suspension cells, such as EL4 Cells, is a problem in the laboratory routine and further in tissue engineering and gene therapy. Most methods working effectively for adherent cells in culture fail to transfect suspension cells.
EL4 Cell Avalanche™ Transfection Reagent is a new class of unique chemical formulations specifically formulated and optimized for transfecting EL4 Cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for EL4 Cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?
Features:
- Highest efficiency to ensure experimental success
- Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
- Easy-to-use protocol: Just mix nucleic acids with the reagent to form complexes and simply add the mixture to cells
- Compatible with serum
- Compatible with transfection in any plate formats
- Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.
- Developed and manufactured by EZ Biosystems
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| EL4 Cell Avalanche™ Transfection Reagent (mouse lymphoma cell) can also be used on the following cells with high transfection efficiencies. 786-O Cell Caki-1 Cell MDCK Cell Vero Cell 293 Cell MRC-5 Cell IMR-90 Cell COS-7 Cell Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list. |
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