Transfection - EZ Biosystems: Cell Lines
Avalanche®-Cell Type / Cell Line-specific Transfection Reagent series:
Perform maximum transfection efficiency for more than 170 different primary cells and cell lines including 124 cancer cell lines (e.g. All of NCI60 cancer cell lines).Each of the cell type/cell line-specific transfection reagents in our Avalanche® series was individually formulated.
For primary cells click HERE
To learn why you should use Avalanche®Cell Type-Specific Transfection reagents, clickHERE; To learn how EZ Biosystems developed Avalanche®Cell Type-Specific Transection Reagents, clickHERE
DLD-1 Cell Avalanche™ Transfection Reagent (human colon adenocarcinoma cell)
DLD-1 Cell Avalanche™ Transfection Reagent is a new class of unique chemical formulations specifically formulated and optimized for transfecting DLD-1 Cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for DLD-1 Cells
EZ Biosystems
| Catalogue No. | Description | Pack Size | Price | Qty |
|
|---|---|---|---|---|---|
| EZT-DLD1-1-05 | DLD-1 CellTransfection Reagent (human colon adenocarcinoma cell | 0.5 ml | £399.00 | Quantity | Add to Order |
| EZT-DLD1-1-15 | DLD-1 CellTransfection Reagent (human colon adenocarcinoma cell | 1.5 ml | £699.00 | Quantity | Add to Order |
DLD-1 Cell Avalanche™ Transfection Reagent (human colon adenocarcinoma cell)
DLD-1 Cell Avalanche™ Transfection Reagent is a new class of unique chemical formulations specifically formulated and optimized for transfecting DLD-1 Cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for DLD-1 Cells
EZ Biosystems
Cell Line Information:
Designations: DLD-1 Depositors: DL Dexter Growth Properties: adherent Organism: Homo sapiens Morphology: epithelial Source: colon Tumor Stage: Dukes" type C Disease: colorectal adenocarcinoma Cell Type: epithelial Cellular Products: carcinoembryonic antigen (CEA) 0.5 ng/10 exp6 cells/10 days; colon antigen 3; keratin Applications: transfection host Tumorigenic: Yes Antigen Expression: blood type O Age: adult Gender:male Comments: DLD-1 is one of two colorectal adenocarcinoma cell lines which were isolated by D.L. Dexter and associates during a period from 1977-1979. The cells are negative for CSAp (CSAp-). DLD-1 cells are positive for p53 antigen expression (the p53 antigen produced has a C -> T mutation resulting in Ser -> Phe at position 241). The cells are positive for keratin by immunoperoxidase staining. The line is positive for expression of c-myc, K-ras, H-ras, N-ras, myb, sis and fos oncogenes.
The Transfection Reagent:
DLD-1 Cell Avalanche™ Transfection Reagent is a new class of unique chemical formulations specifically formulated and optimized for transfecting DLD-1 Cells. The proprietary formulation of lipids and polymersensures the highest possible transfection efficiencies and viabilities for DLD-1 Cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?
Features:
- Specifically optimized to deliver nucleic acids into DLD-1 Cells
- Highest efficiency to ensure experimental success
- Extremely gentle to cells
- Deliver single or multiple plasmids
- Efficient for plasmid DNA/siRNA co-transfection
- Synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cells lines
- Easy-to-use protocol: Just mix nucleic acids with the reagent to form complexes and simply add the mixture to cells
- Economical: High efficiency means less amount of nucleic acid & reagent is needed
- Developed and manufactured by EZ Biosystems
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| DLD-1 Cell Avalanche™ Transfection Reagent (human colon adenocarcinoma cell) can also be used on the following cells with high transfection efficiencies. T84 Cell HCT 116 Cell HT-29 Cell Caco-2 Cell LS 174T Cell LoVo Cell SW480 Cell SW620 Cell 293 Cell Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list. |
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