Transfection - EZ Biosystems: Cell Lines
Avalanche®-Cell Type / Cell Line-specific Transfection Reagent series:
Perform maximum transfection efficiency for more than 170 different primary cells and cell lines including 124 cancer cell lines (e.g. All of NCI60 cancer cell lines).Each of the cell type/cell line-specific transfection reagents in our Avalanche® series was individually formulated.
For primary cells click HERE
To learn why you should use Avalanche®Cell Type-Specific Transfection reagents, clickHERE; To learn how EZ Biosystems developed Avalanche®Cell Type-Specific Transection Reagents, clickHERE
BT-20 Cell Avalanche™ Transfection Reagent (human breast carcinoma cell)
BT-20 Cell Avalanche™ Transfection Reagent is a new, proprietary solution specifically designed for transfection on BT-20 Cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for BT-20 Cells. No other transfection reagents can match the efficiency, convenience, and gentleness of BT-20 Cell Avalanche™ Transfection Reagent for transfection on BT-20 Cells.
EZ Biosystems
| Catalogue No. | Description | Pack Size | Price | Qty |
|
|---|---|---|---|---|---|
| EZT-BT20-1-05 | BT-20 Cell Transfection Reagent (human breast carcinoma cell) | 0.5 ml | £399.00 | Quantity | Add to Order |
| EZT-BT20-1-15 | BT-20 Cell Transfection Reagent (human breast carcinoma cell) | 1.5 ml | £699.00 | Quantity | Add to Order |
BT-20 Cell Avalanche™ Transfection Reagent (human breast carcinoma cell)
BT-20 Cell Avalanche™ Transfection Reagent is a new, proprietary solution specifically designed for transfection on BT-20 Cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for BT-20 Cells. No other transfection reagents can match the efficiency, convenience, and gentleness of BT-20 Cell Avalanche™ Transfection Reagent for transfection on BT-20 Cells.
EZ Biosystems
Cell Line Information:
Designations: BT-20 Depositors: EY Lasfargues Growth Properties: adherent Organism: Homo sapiens Morphology: epithelial Source:Organ: mammary gland; breast Disease: carcinoma Isolation date: 1958 Tumorigenic: Yes Antigen Expression: HLA A1, Bw16 ( /-) Age: 74 years Gender:female Ethnicity: Caucasian Comments: The cells express the WNT3 and the WNT7B oncogenes. This breast tumor line was established by E.Y. Lasfargues and L. Ozzello in 1958 by isolation and cultivation of cells spilling out of the tumor when it was cut in thin slices. Growth of BT-20 cells is inhibited by tumor necrosis factor alpha (TNF alpha). BT-20 cells are negative for estrogen receptor, but do express an estrogen receptor mRNA that has deletion of exon 5.
The Transfection Reagent:
BT-20 Cell Avalanche™ Transfection Reagent is a new, proprietary solution specifically designed for transfection on BT-20 Cells. The proprietary formulation of lipids and polymersensures the highest possible transfection efficiencies and viabilities for BT-20 Cells. No other transfection reagents can match the efficiency, convenience, and gentleness of BT-20 Cell Avalanche™ Transfection Reagent for transfection on BT-20 Cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?
Features:
- Specifically optimized to deliver nucleic acids into BT-20 Cells
- Highest efficiency to ensure experimental success
- Extremely gentle to cells
- Efficient for plasmid DNA/siRNA co-transfection
- Easy-to-use protocol: Just mix nucleic acids with the reagent to form complexes and simply add the mixture to cells
- Compatible with serum
- Suitable for Reverse Transfection
- Compatible with transfection in any plate formats
- Economical: High efficiency means less amount of nucleic acid & reagent is needed
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| BT-20 Cell Avalanche™ Transfection Reagent (human breast carcinoma cell) can also be used on the following cells with high transfection efficiencies. 4T1 Cell ZR-75-1 Cell MDA-MB-453 Cell HCC1937 Cell MDA-MB-468 Cell SK-BR-3 Cell MDA-MB-231 Cell 293 Cell 293T/17 Cell Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list. |
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