Transfection - EZ Biosystems: Cell Lines
Avalanche®-Cell Type / Cell Line-specific Transfection Reagent series:
Perform maximum transfection efficiency for more than 170 different primary cells and cell lines including 124 cancer cell lines (e.g. All of NCI60 cancer cell lines).Each of the cell type/cell line-specific transfection reagents in our Avalanche® series was individually formulated.
For primary cells click HERE
To learn why you should use Avalanche®Cell Type-Specific Transfection reagents, clickHERE; To learn how EZ Biosystems developed Avalanche®Cell Type-Specific Transection Reagents, clickHERE
BALB/3T3 clone A31 Avalanche™ Transfection Reagent
BALB/3T3 clone A31 Avalanche™ Transfection Reagent is a new class of unique chemical formulations specifically formulated and optimized for transfecting BALB/3T3 clone A31 Cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for BALB/3T3 clone A31 Cells.
EZ Biosystems
| Catalogue No. | Description | Pack Size | Price | Qty |
|
|---|---|---|---|---|---|
| EZT-BALB-1-05 | BALB/3T3 clone A31 ransfection Reagent | 0.5ml | £399.00 | Quantity | Add to Order |
| EZT-BALB-1-15 | BALB/3T3 clone A31 ransfection Reagent | 1.5ml | £699.00 | Quantity | Add to Order |
BALB/3T3 clone A31 Avalanche™ Transfection Reagent
BALB/3T3 clone A31 Avalanche™ Transfection Reagent is a new class of unique chemical formulations specifically formulated and optimized for transfecting BALB/3T3 clone A31 Cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for BALB/3T3 clone A31 Cells.
EZ Biosystems
Cell line Informatrion:
Designations: BALB/3T3 clone A31 Depositors: S Aaronson Growth Properties: adherent Organism: Mus musculus Morphology: fibroblast Source: embryo Strain: BALB/c Cell Type: fibroblast Isolation date: 1968 Applications: transfection host Virus Susceptibility: Human poliovirus 1, Herpes simplex virus, Vesicular stomatitis virus Tumorigenic: No Age: embryo; 14 to 17 day gestation The BALB/3T3 clone A31 is one of several cell lines developed by S.A. Aaronson and G.T. Todaro in 1968 from disaggregated 14- to 17-day-old BALB/c mouse embryos. The cells are extremely sensitive to contact inhibition of cell division, grow at a high dilution, exhibit a low saturation density and are highly susceptible to transformation in tissue culture by the oncogenic DNA virus SV40 and murine sarcoma virus.
The Transfection Reagent:
BALB/3T3 clone A31 Avalanche™ Transfection Reagent is a new class of unique chemical formulations specifically formulated and optimized for transfecting BALB/3T3 clone A31 Cells. The proprietary formulation of lipids and polymersensures the highest possible transfection efficiencies and viabilities for BALB/3T3 clone A31 Cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?
Features:
- Specifically optimized to deliver nucleic acids into BALB/3T3 clone A31 Cells
- Highest efficiency to ensure experimental success
- Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
- Synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cells lines
- Easy-to-use protocol: Just mix nucleic acids with the reagent to form complexes and simply add the mixture to cells
- Compatible with serum
- Suitable for Reverse Transfection
- Compatible with transfection in any plate formats
- Economical: High efficiency means less amount of nucleic acid & reagent is needed
- Developed and manufactured by EZ Biosystems
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| BALB/3T3 clone A31 Avalanche™ Transfection Reagent can also be used on the following cells with high transfection efficiencies. NIH/3T3 Cell 3T3-L1 Cell 3T3-Swiss albino Cell MDCK Cell Vero Cell 293 Cell MRC-5 Cell IMR-90 Cell COS-7 Cell BHK-21 Cell 3197-3 Cell Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list. |
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