PCR : PCR Products
The FailSafe™ PCR system for templates up to 20kb long, high GC content (up to 85% G+C), & Multiplex PCR
Reverse Transcriptase (HIV)
Human Immunodeficiency Virus (HIV) Reverse Transcriptase is an RNA directed DNA polymerase which can synthesize a complementary DNA strand initiating from a primer using either RNA or single-stranded DNA as a template.
Cambio
| Catalogue No. | Description | Pack Size | Price | Qty |
|
|---|---|---|---|---|---|
| PM-1373-01 | Reverse Transcriptase (HIV) | 500U | POA | Quantity | Add to Order |
Reverse Transcriptase (HIV)
Human Immunodeficiency Virus (HIV) Reverse Transcriptase is an RNA directed DNA polymerase which can synthesize a complementary DNA strand initiating from a primer using either RNA or single-stranded DNA as a template.
Cambio
Description
- Human Immunodeficiency Virus (HIV) Reverse Transcriptase is an RNA directed DNA polymerase which can synthesize a complementary DNA strand initiating from a primer using either RNA or single-stranded DNA as a template.
Technical Datasheet available here
Applications
- Provides an excellent target for evaluating antiviral agents or inhibitors (1,2)
- Catalyzes error-prone synthesis on DNA and RNA templates - ideal for introducing random mutations (3)
Unit Definition
One unit is the amount of enzyme required to incorporate 1 nmol of labelled dTTP into acid insoluble material in 10 minutesat 37°C.
Concentration
10 - 30 units/µl
Assay Conditions
50 mM Tris-HCl (pH 8.3) 6 mM MgCl240 mM KCl0.5 mM [3H] TTP0.4 mM poly rA:dTReaction volume of 50 µl
Storage Buffer
20 mM potassium phosphate (pH 7.1) 1.0 mM dithiothreitol0.02% Triton X-100™50% (v/v) glycerol
Reaction Buffer
10X HIV-RT Reaction Buffer
Storage Conditions
Store at -20°CProduct shipped on Dry Ice
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
Reverse Transcriptase (HIV)
Human Immunodeficiency Virus (HIV) Reverse Transcriptase is an RNA directed DNA polymerase which can synthesize a complementary DNA strand initiating from a primer using either RNA or single-stranded DNA as a template.
Cambio
Click here for Data sheet
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
Reverse Transcriptase (HIV)
Human Immunodeficiency Virus (HIV) Reverse Transcriptase is an RNA directed DNA polymerase which can synthesize a complementary DNA strand initiating from a primer using either RNA or single-stranded DNA as a template.
Cambio
References
1. Hirsch, M. S., Kaplan, J. C. (1985) Ann. Intern. Med. 103, 750-7552. Tisdale, M. et al (1989) J. Antimicrob. Chemother. 23, 47-543. Williams, K. J., Loeb, L. A. (1992) Curr. Top. Microbiol. Immunol. 176, 80-165
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
Reverse Transcriptase (HIV)
Human Immunodeficiency Virus (HIV) Reverse Transcriptase is an RNA directed DNA polymerase which can synthesize a complementary DNA strand initiating from a primer using either RNA or single-stranded DNA as a template.
Cambio
Applications
- Provides an excellent target for evaluating antiviral agents or inhibitors (1,2)
- Catalyzes error-prone synthesis on DNA and RNA templates - ideal for introducing random mutations (3)
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
