In Vitro Toxicology: Mutagenicity kits
The Ames II / Ames MPF Microfluctuation Assays are all modifications of the traditional Ames plate incorporation assay. The Ames II assay uses the S. typhimurium strains TA98 and TAMix (a mixture of 6 strains, each detecting a specific base-pair substitution), whereas the Ames MPF® kits are available with the strains TA98, TA100, TA1535, TA1537, E.coli WP2 uvrA or E.coli WP2 pKM101.
Benefits of Xenometrix Ames Mutagenicity Assays over traditional Ames Procedures
Easy, compact and high throughput liquid format with 384 well plates.
Simple detection method with easy "readout" (as compared to the traditional assay)
Kits include all five types strains cited in OECD 471 (CoA supplied), quality controlled according to the same guideline
Much less test compound required. (10 mg per strain if tested in triplicates, +/- S9, 6 semi-logarithmic compound dilutions)
Ames MPF is the preferred system for genotoxic impurities due to its low consumption of compound
Significantly less consumption of S9
Less environmental pollution due to significantly less "contaminated" waste
Less user intervention
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Ames II Test Kits
Rapid screening:The Ames II assay comprises the frameshift strain TA98, and TAMix, consisting of 6 histidine auxotrophic Salmonella tester strains, TA7001-TA7006.
Xenometrix AG
| Catalogue No. | Description | Pack Size | Price | Qty |
|
|---|---|---|---|---|---|
| XE10-213 | Ames II - 10 Sample Kit - 480 Measuring Points / Strain | 10 sample kit | £1,730.00 | Quantity | Add to Order |
| XE10-213-S1-P | Ames II - 10 Sample Kit with Rat Liver S9 (aroclor) and Positive Controls | 10 sample kit | £2,070.00 | Quantity | Add to Order |
Ames II Test Kits
Rapid screening:The Ames II assay comprises the frameshift strain TA98, and TAMix, consisting of 6 histidine auxotrophic Salmonella tester strains, TA7001-TA7006.
Xenometrix AG
Rapid screening:
The Ames II assay comprises the frameshift strain TA98, and TAMix, consisting of 6 histidine auxotrophic Salmonella tester strains, TA7001-TA7006. Each of these mutants can be reverted only by a specific transition or transversion, so that all possible base-pair changes can be detected by TAMix.
TA98 and TAMix may be used for mutagenicity screening because they indicate both frameshift and base-pair mutations.
The TA700x tester strains are not included among the recommended tester strains in the current, formal test guidelines. The Xenometrix Ames MPF 98/100 detects a similar mutational spectrum but contains the traditional Ames tester strains TA98 and TA100.
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