Oligo Synthesis : CEPs
Prices quoted are for single packs only. For multiples of the same product please request a quote. Some of Glen"sproductsarehazardousandmay be subject to additional shipping charges. Full product information is available onGlen Research"s website.
Zebularine-CE Phosphoramidite
Zebularine-CE Phosphoramidite
Glen Research
| Catalogue No. | Description | Pack Size | Price | Qty |
|
|---|---|---|---|---|---|
| 10-3011-02 | Zebularine-CE Phosphoramidite | 0.25g | £520.00£494.00Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 10-3011-90 | Zebularine-CE Phosphoramidite | 100µmoles | £200.00£190.00Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 10-3011-95 | Zebularine-CE Phosphoramidite | 50µmoles | £100.00£95.00Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
Related products
Zebularine-CE Phosphoramidite
Zebularine-CE Phosphoramidite
Glen Research
| Zebularine-CE Phosphoramidite |
Catalog Number: 10-3011-xx
Description: Zebularine-CE Phosphoramidite
| 5"-Dimethoxytrityl-pyrimidin-2-one-riboside, 2"-O-TBDMS-3"-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite | ||
| Formula: C45H61N4O8PSi | M.W.: 845.05 | F.W.: 290.17 |
Diluent: Anhydrous Acetonitrile |
| Coupling: 12 minute coupling time is recommended. Monomers that allow for UltraMild deprotection must be used. To avoid any exchange of the iPr-Pac group on the dG with acetyl, use the UltraMild Cap Mix A (40-4210-xx/ 40-4212-xx). |
| Deprotection: Cleave and remove base protection in 30% Ammonium Hydroxide:EtOH (3:1) for 4 hours at Room Temperature. Complete 2" deprotection per RNA Technical Bulletin. See Technical Bulletin for details (Technical Bulletin). |
| Storage: Freezer storage, -10 to -30°C, dry |
| Stability in Solution: 1-2 days |
7-Deaza-Adenosine is lacking nitrogen at the 7-position, which is replaced by carbon. The N7 position in adenosine takes part in non-Watson and Crick hydrogen bonding, which may be relevant to RNA folding and subsequent activity. This Adenosine analogue is also known as Tubercidin. 8-Aza-7-deaza-Adenosine is an isomer of Adenosine with virtually identical electron density. Again, the N7 nitrogen is not available for hydrogen bonding. Nebularine or Purine Nucleoside can be viewed as an Adenosine derivative that is lacking the exocyclic amino group. This molecule allows researchers to determine the relevance of the exocyclic amine of Adenosine to RNA structure and function. Ribozyme activity is substantially affected by the substitution of modified pyrimidine bases. Zebularine (pyrimidin-2-one ribonucleoside) may be regarded as a Cytidine derivative lacking the exocyclic amino group. Zebularine and Pyridin-2-one Ribonucleoside, the 3-deaza analogue of Zebularine, are prime candidates for use in evaluating ribozyme activity and function. It should be noted that Zebularine is mildly fluorescent, absorbing at 298nm and emitting at 367nm. PseudoUridine is one of the most common modified nucleosides found in RNA. The availability of a phosphoramidite will allow detailed research into the effects of this modified base on RNA structure and activity.
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
Zebularine-CE Phosphoramidite
Zebularine-CE Phosphoramidite
Glen Research
Material Safety Data Sheet
Glen Report 19.1: Modified RNA Phosphoramidites Useful In Sirna Research And Biologically Significant 1-Methy-Adenosine
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
Zebularine-CE Phosphoramidite
Zebularine-CE Phosphoramidite
Glen Research
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link formore detailed usage informationwith the various synthesizers.
| ABI 392/394 | |||||||||
| Cat.No. | PackSize | Grams/Pack | 0.1M Dil.(mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 10-3011-95 | 50µmoles | .042grams | .5 | 3.33 | 2 | 1.25 | .91 | .67 | .17 |
| 10-3011-90 | 100µmoles | .085grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| 10-3011-02 | 0.25grams | .25grams | 2.96 | 85.33 | 51.2 | 32 | 23.27 | 17.07 | 4.27 |
| Expedite | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 10-3011-95 | 50µmoles | .042grams | .75 | .07 | N/A | N/A | 1.79 | N/A | |
| 10-3011-90 | 100µmoles | .085grams | 1.5 | .07 | N/A | N/A | 4.92 | N/A | |
| 10-3011-02 | 0.25grams | .25grams | 4.42 | .07 | N/A | N/A | 17.08 | N/A | |
| Beckman | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 10-3011-95 | 50µmoles | .042grams | .75 | .07 | N/A | 1.65 | 1.55 | ||
| 10-3011-90 | 100µmoles | .085grams | 1.5 | .07 | N/A | 4.06 | 3.82 | ||
| 10-3011-02 | 0.25grams | .25grams | 4.42 | .07 | N/A | 13.48 | 12.67 | ||
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
