Oligo Synthesis : CEPs
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UniCap Phosphoramidite
UniCap Phosphoramidite
Glen Research
| Catalogue No. | Description | Pack Size | Price | Qty |
|
|---|---|---|---|---|---|
| 10-4410-02 | UniCap Phosphoramidite | 0.25g | £40.00£38.00Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 10-4410-05 | UniCap Phosphoramidite | 0.5g | £80.00£76.00Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 10-4410-10 | UniCap Phosphoramidite | 1.0g | £160.00£152.00Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 10-4410-20 | UniCap Phosphoramidite | 2.0g | £320.00£304.00Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
Related products
UniCap Phosphoramidite
UniCap Phosphoramidite
Glen Research
| UniCap Phosphoramidite |
Catalog Number: 10-4410-xx
Description: UniCap Phosphoramidite
| Diethyleneglycol ethyl ether (2-cyanoethyl)-(N,N-diisopropyl)-phosphoramidite | ||
| Formula: C15H31N2O4P | M.W.: 334.39 | |
Diluent: Anhydrous AcetonitrileAdd fresh diluent to product vial to recommended concentration and swirl vial occasionally over several minutes until product is completely dissolved. (Some oils may require between 5 and 10 minutes.) Use care to maintain anhydrous conditions. In case of transfer to alternate vial type, ensure recipient vial has been pre-dried. For more information, seehttp://www.glenres.com/Technical/TB_ABITransfer.pdf. |
| Coupling: For capping, add as a second coupling step after base coupling using standard coupling conditions. |
| Deprotection: No changes needed from standard method recommended by synthesizer manufacturer. |
| Storage: Freezer storage, -10 to -30°C, dry |
| Stability in Solution: 7 days |
| Please Note: This product is supplied under license. |
ALTERNATIVE SOLVENTS/REAGENTS
Glen Research offers alternative solvents and reagents in suitable bottles and formulations for use on various DNA synthesizers. All solvents and reagents are prepared to our exacting specifications to ensure the highest coupling efficiencies and are passed through a 0.2 micron filter during packaging to eliminate particulate contamination. Glen Research offers the activators below in powder form for later dissolution in anhydrous acetonitrile or as a prepared solution.
ALTERNATIVE CAPPING REAGENT
The phosphoramidite of diethylene glycol monoethyl ether, UniCap, is the basis for an alternative capping reagent. To use UniCap as a capping amidite on the Expedite 8909 or AB synthesizers, dilute it to the standard amidite concentration and place the vial in position 5 on the instrument. Cycles can be modified by adding coupling steps for amidite reservoir 5 after the last column coupling step. The standard capping steps can be left out of the cycle. UniCap Phosphoramidite was originally developed for oligo synthesis on the surface of chips and is the capping reagent of choice for this application.
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
UniCap Phosphoramidite
UniCap Phosphoramidite
Glen Research
Material Safety Data Sheet
??Glen Report 17.1: UniCap Phosphoramidite, An alternative to acetic anhydride capping
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
UniCap Phosphoramidite
UniCap Phosphoramidite
Glen Research
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link formore detailed usage informationwith the various synthesizers.
| ABI 392/394 | |||||||||
| Cat.No. | PackSize | Grams/Pack | 0.1M Dil.(mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 10-4410-02 | 0.25grams | .25grams | 7.48 | 236 | 141.6 | 88.5 | 64.36 | 47.2 | 11.8 |
| 10-4410-05 | 0.5grams | .5grams | 14.95 | 485 | 291 | 181.88 | 132.27 | 97 | 24.25 |
| 10-4410-10 | 1.0gram | 1grams | 29.91 | 983.67 | 590.2 | 368.88 | 268.27 | 196.73 | 49.18 |
| 10-4410-20 | 2.0grams | 2grams | 59.81 | 1980.33 | 1188.2 | 742.63 | 540.09 | 396.07 | 99.02 |
| Expedite | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 10-4410-02 | 0.25grams | .25grams | 11.16 | .07 | 216.8 | 135.5 | 98.55 | 13.55 | |
| 10-4410-05 | 0.5grams | .5grams | 22.32 | .07 | 440 | 275 | 200 | 27.5 | |
| 10-4410-10 | 1.0gram | 1grams | 44.63 | .07 | 886.2 | 553.88 | 402.82 | 55.39 | |
| 10-4410-20 | 2.0grams | 2grams | 89.27 | .07 | 1779 | 1111.88 | 808.64 | 111.19 | |
| Beckman | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 10-4410-02 | 0.25grams | .25grams | 11.16 | .07 | 218.4 | 136.5 | 99.27 | ||
| 10-4410-05 | 0.5grams | .5grams | 22.32 | .07 | 441.6 | 276 | 200.73 | ||
| 10-4410-10 | 1.0gram | 1grams | 44.63 | .07 | 887.8 | 554.88 | 403.55 | ||
| 10-4410-20 | 2.0grams | 2grams | 89.27 | .07 | 1780.6 | 1112.88 | 809.36 | ||
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
