Oligo Synthesis : CEPs
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Thymidine Glycol CE Phosphoramidite
Thymidine Glycol CE Phosphoramidite
Glen Research
| Catalogue No. | Description | Pack Size | Price | Qty |
|
|---|---|---|---|---|---|
| 10-1096-02 | Thymidine Glycol CE Phosphoramidite | 0.25g | £780.00£741.00Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 10-1096-90 | Thymidine Glycol CE Phosphoramidite | 100µmoles | £332.00£273.60Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 10-1096-95 | Thymidine Glycol CE Phosphoramidite | 50µmoles | £172.00£136.80Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
Related products
Thymidine Glycol CE Phosphoramidite
Thymidine Glycol CE Phosphoramidite
Glen Research
| Thymidine Glycol CE Phosphoramidite |
Catalog Number: 10-1096-xx
Description: Thymidine Glycol CE Phosphoramidite
| 5"-Dimethoxytrityl-2"-deoxy-(5R,6S)-5,6-bis(t-butyldimethylsilyloxy)-5,6-dihydroThymidine,3"-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite | ||
| Formula: C52H79N4O10PSi2 | M.W.: 1007.36 | F.W.: 338.21 |
Diluent: Anhydrous Acetonitrile |
| Coupling: 3 minute coupling time is recommended. Monomers that allow for UltraMILD deprotection must be used. (Catalog Numbers: dA: 10-1601-xx, dC: 10-1015-xx, dG: 10-1621-xx, dT: 10-1030-xx.). To avoid any exchange of the iPr-Pac group on the dG with acetyl, use the UltraMild Cap Mix A (40-4210-xx/ 40-4212-xx). |
| Deprotection: Cleave and deprotect the oligonucleotide in 30% Ammonium Hydroxide for 2 hours at Room Temperature. Dry down and dissolve the residue in 0.5 mL of TEA.3HF and keep at 40° C overnight to remove the TBDMS protecting groups. Add an equal volume of dH2O and desalt on a Glen Gel-Pak™ column or equivalent. |
| Storage: Freezer storage, -10 to -30°C, dry |
| Stability in Solution: 2-3 days |
8-Amino-G is formed along with 8-oxo-G as the major mutagenic lesions formed in DNA damage caused by 2-nitropropane. 2-Nitropropane is an industrial solvent and a component of paints, dyes and varnishes, and is also present in cigarette smoke. Thymine glycol (5,6-dihydroxy-5,6-dihydrothymine) is formed when thymine is subjected to oxidative stress, including ionizing radiation. Oxidation of the 5,6 double bond of Thymidine generates two chiral centers at C5 and C6. The cis-5R,6S form is generated as the predominant product along with the other diastereomer, the cis-5S,6R form. The presence of thymidine glycol in DNA has significant biological consequences and many organisms possess specific repair enzymes for the excision of this lesion. 2-Aminoimidazolone (Iz) and its hydrolysis product imidazolone (Z) are major oxidation products of G. Access to these two potential lesions is not possible during oligonucleotide synthesis because they are so base-labile. A suitable precursor, 8-methoxy-dG (8-OMe-dG), to dIz has now been described. The conversion of 8-OMe-dG to dIz takes place by irradiation of the oligonucleotide (1 mM) in 50 mM sodium cacodylate buffer, pH 7, in the presence of riboflavin (50 µM) for 2 minutes on a transilluminator (366 nm), under aerobic conditions at 4°C. Surprisingly for a photochemical reaction, the conversion is virtually quantitative.
Hydrolysis of nucleoside residues in DNA occurs to generate abasic sites. Most commonly, dA sites are hydrolyzed causing depurination and leading to abasic residues. For researchers trying to determine if their source of depurination in chemical synthesis of DNA is reagent, fluidics or protocol-based, we offer a depurination-resistant dA monomer. A new chemical method allows the generation of abasic sites in double and single stranded oligonucleotides using very mild specific conditions and with very low probability of side reactions. The original Abasic Phosphoramidite (10-1924) has been discontinued since it exhibits low coupling efficiency and the post-synthesis chemistry is fairly challenging. Abasic II Phosphoramidite1 is the replacement for the preparation of a true abasic site. This product has the advantage of simplicity in that the silyl group is removed post-synthesis using aqueous acetic acid. dSpacer has also been used successfully as a mimic of the highly base-labile abasic site.
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Thymidine Glycol CE Phosphoramidite
Thymidine Glycol CE Phosphoramidite
Glen Research
Material Safety Data Sheet
Glen Report 16.1: Minor Base and Related Novel Phosphoramidites
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Thymidine Glycol CE Phosphoramidite
Thymidine Glycol CE Phosphoramidite
Glen Research
Oligonucleotides synthesised using Thymidine Glycol and UltraMILD monomers can be cleaved using either concentrated ammonium hydroxide or 50mM potassium carbonate in anhydrous methanol. Complete cleavage and deprotection can be accomplished at room temperature in 2-4 hours without damaging Thymidine Glycol base. The best method to remove teh TBDMS groups was achieved using TEA.3HF at 40°C overnight.
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Thymidine Glycol CE Phosphoramidite
Thymidine Glycol CE Phosphoramidite
Glen Research
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link formore detailed usage informationwith the various synthesizers.
| ABI 392/394 | |||||||||
| Cat.No. | PackSize | Grams/Pack | 0.1M Dil.(mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 10-1096-02 | 0.25grams | .25grams | 2.48 | 69.33 | 41.6 | 26 | 18.91 | 13.87 | 3.47 |
| 10-1096-90 | 100µmoles | .101grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| 10-1096-95 | 50µmoles | .05grams | .5 | 3.33 | 2 | 1.25 | .91 | .67 | .17 |
| Expedite | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 10-1096-02 | 0.25grams | .25grams | 3.7 | .07 | 67.6 | 42.25 | 30.73 | 4.23 | |
| 10-1096-90 | 100µmoles | .101grams | 1.5 | .07 | 23.6 | 14.75 | 10.73 | 1.48 | |
| 10-1096-95 | 50µmoles | .05grams | .75 | .07 | 8.6 | 5.38 | 3.91 | .54 | |
| Beckman | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 10-1096-02 | 0.25grams | .25grams | 3.7 | .07 | 69.2 | 43.25 | 31.45 | ||
| 10-1096-90 | 100µmoles | .101grams | 1.5 | .07 | 25.2 | 15.75 | 11.45 | ||
| 10-1096-95 | 50µmoles | .05grams | .75 | .07 | 10.2 | 6.38 | 4.64 | ||
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