Oligo Synthesis : CEPs
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tCnitro-CE Phosphoramidite
tCnitro-CE Phosphoramidite
Glen Research
| Catalogue No. | Description | Pack Size | Price | Qty |
|
|---|---|---|---|---|---|
| 10-1518-02 | tCnitro-CE Phosphoramidite | 0.25g | £1,168.00£1,109.60Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 10-1518-90 | tCnitro-CE Phosphoramidite | 100umole | £416.00£395.20Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 10-1518-95 | tCnitro-CE Phosphoramidite | 50umole | £212.00£201.40Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
Related products
tCnitro-CE Phosphoramidite
tCnitro-CE Phosphoramidite
Glen Research
Catalog Number: 10-1518-xx
Description: tCnitro-CE Phosphoramidite
| 5"-O-(4,4"-Dimethoxytrityl)-1"-(7-nitro-1,3-diaza-2-oxophenothiazin-1-yl)-2"-deoxy-Β-D-ribofuranosyl-3"-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite | ||
| Formula: C45H49N6O9PS | M.W.: 880.94 | F.W.: 440.32 |
Diluent: Anhydrous Acetonitrile |
| Coupling: No changes needed from standard method recommended by synthesizer manufacturer. |
| Deprotection: As required by nucleobases. Compatible with Ammonium Hydroxide for 17 hours at room temperature, Ammonium hydroxide/40% methylamine 1:1 (AMA) for 10 minutes at 65°C or UltraMild deprotection conditions. |
| Storage: Freezer storage, -10 to -30°C, dry |
| Stability in Solution: 1-2 days |
Please Note: These products are offered under license from ISIS Pharmaceuticals, Inc. and in collaboration with ModyBase HB. The tricyclic fluorescent nucleoside analogues, 1,3-diaza-2-oxophenothiazine, tC, and 1,3-diaza-2-oxophenoxazine, tCo, are deoxycytidine analogs that have been shown to base pair faithfully with dG with virtually no disruption of the normal duplex structure. This means that the stability of the DNA duplex is not compromised as compared to the control regardless of DNA sequence. The fluorescence quantum yield of tC is essentially unchanged between single stranded and double stranded DNA - 0.21 for single stranded DNA and 0.19 for duplex DNA. Also, the fluorescence characteristics of tC are not sensitive to neighboring base combinations. tCo has been shown to be the brightest fluorescent nucleoside analogue in duplex context reported so far and even retains the majority of its fluorescence when surrounded by guanine residues. Indeed, tCo has been reported to be 25-50 times brighter than 2-aminopurine. |
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tCnitro-CE Phosphoramidite
tCnitro-CE Phosphoramidite
Glen Research
Material Safety Data Sheet
Glen Report 22.1: tC, tCo and tCnitro: Tricyclic Cytosine Probes of DNA and RNA Structure
Glen Report 24.2: Technical Brief: FRETmatrix - A Method for Simulation and Analysis of FRET in Oligos
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tCnitro-CE Phosphoramidite
tCnitro-CE Phosphoramidite
Glen Research
Glen Report 22.1: tC, tCo and tCnitro: Tricyclic Cytosine Probes of DNA and RNA Structure
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tCnitro-CE Phosphoramidite
tCnitro-CE Phosphoramidite
Glen Research
Please Note: These products are offered under license from ISIS Pharmaceuticals, Inc. and in collaboration with ModyBase HB.
Absorption and emisssion data for tC and tCo arecollected below:
tC | QY | l | e(L/mol.cm) |
single-stranded | 0.21 | 385nm | |
double-stranded | 0.19 | ||
tCo | QY | l | e(L/mol.cm) |
single-stranded | 0.30 | 360nm | 9000 |
double-stranded | 0.21 |
(QY determined relative to quinine sulfate in 0.5M H2SO4)
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tCnitro-CE Phosphoramidite
tCnitro-CE Phosphoramidite
Glen Research
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.
| ABI 392/394 | |||||||||
| Cat.No. | PackSize | Grams/Pack | 0.1M Dil.(mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 10-1518-95 | 50µmoles | .044grams | .5 | 3.33 | 2 | 1.25 | .91 | .67 | .17 |
| 10-1518-90 | 100µmoles | .088grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| 10-1518-02 | 0.25grams | .25grams | 2.84 | 81.33 | 48.8 | 30.5 | 22.18 | 16.27 | 4.07 |
| Expedite | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 10-1518-95 | 50µmoles | .044grams | .75 | .07 | 8.6 | 5.38 | 3.91 | .54 | |
| 10-1518-90 | 100µmoles | .088grams | 1.5 | .07 | 23.6 | 14.75 | 10.73 | 1.48 | |
| 10-1518-02 | 0.25grams | .25grams | 4.24 | .07 | 78.4 | 49 | 35.64 | 4.9 | |
| Beckman | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 10-1518-95 | 50µmoles | .044grams | .75 | .07 | 10.2 | 6.38 | 4.64 | ||
| 10-1518-90 | 100µmoles | .088grams | 1.5 | .07 | 25.2 | 15.75 | 11.45 | ||
| 10-1518-02 | 0.25grams | .25grams | 4.24 | .07 | 80 | 50 | 36.36 | ||
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