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Athens Research/Cambio - Excellence in Molecular Biology/10-1516-02

商品编号： 10-1516-02

品牌： Athens Research & Technology

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产地：美国(厂家直采)

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产品分类：多肽合成

公司分类： peptide

联系Q Q： 3392242852

电话号码： 4000-520-616

电子邮箱： info@ebiomall.com

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商品介绍

Oligo Synthesis : CEPs

Prices quoted are for single packs only. For multiples of the same product please request a quote. Some of Glen"sproductsarehazardousandmay be subject to additional shipping charges. Full product information is available onGlen Research"s website.

tC-CE Phosphoramidite

Glen Research

Catalogue No.	Description	Pack Size	Price	Qty	Change to: €
10-1516-02	tC-CE Phosphoramidite	0.25g	£1,168.00£1,109.60Offer until : 31-Dec-2020Offer Code : GLEN55% off all Glen productsView Offer	Quantity	Add to Order
10-1516-90	tC-CE Phosphoramidite	100µmoles	£392.00£372.40Offer until : 31-Dec-2020Offer Code : GLEN55% off all Glen productsView Offer	Quantity	Add to Order
10-1516-95	tC-CE Phosphoramidite	50µmoles	£200.00£190.00Offer until : 31-Dec-2020Offer Code : GLEN55% off all Glen productsView Offer	Quantity	Add to Order

tC-CE Phosphoramidite

Glen Research

STructure

Catalog Number: 10-1516-xx

Description: tC-CE Phosphoramidite

5"-O-(4,4"-Dimethoxytrityl)-1"-(1,3-diaza-2-oxophenothiazin-1-yl)-2"-deoxy-Β-D-ribofuranosyl-3"-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
Formula: C₄₅H₅₀N₅O₇PS	M.W.: 835.95	F.W.: 395.33

Diluent: Anhydrous Acetonitrile

Couping: 3 minute coupling time.

Deprotection: As required by nucleobases. Compatible with Ammonium Hydroxide for 17 hours at room temperature, Ammonium hydroxide/40% methylamine 1:1 (AMA) for 10 minutes at 65°C or UltraMild deprotection conditions.

Storage: Freezer storage, -10 to -30°C, dry

Stability in Solution: 1-2 days

Please Note: These products are offered under license from ISIS Pharmaceuticals, Inc. and in collaboration with ModyBase HB.

The tricyclic fluorescent nucleoside analogues, 1,3-diaza-2-oxophenothiazine, tC, and 1,3-diaza-2-oxophenoxazine, tCo, are deoxycytidine analogs that have been shown to base pair faithfully with dG with virtually no disruption of the normal duplex structure. This means that the stability of the DNA duplex is not compromised as compared to the control regardless of DNA sequence. The fluorescence quantum yield of tC is essentially unchanged between single stranded and double stranded DNA - 0.21 for single stranded DNA and 0.19 for duplex DNA. Also, the fluorescence characteristics of tC are not sensitive to neighboring base combinations. tCo has been shown to be the brightest fluorescent nucleoside analogue in duplex context reported so far and even retains the majority of its fluorescence when surrounded by guanine residues. Indeed, tCo has been reported to be 25-50 times brighter than 2-aminopurine.

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

tC-CE Phosphoramidite

Glen Research

Material Safety Data Sheet

Glen Report 21.2: tC and tC^o: New Tricyclic Fluorescent Cytidine Analogues with a very Bright Future

Glen Report 22.1: tC, tCo and tCnitro: Tricyclic Cytosine Probes of DNA and RNA Structure

Glen Report 24.2: Technical Brief: FRETmatrix - A Method for Simulation and Analysis of FRET in Oligos

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

tC-CE Phosphoramidite

Glen Research

Glen Report 21.2: tC and tC^o: New Tricyclic Fluorescent Cytidine Analogues with a very Bright Future

Glen Report 22.1: tC, tCo and tCnitro: Tricyclic Cytosine Probes of DNA and RNA Structure

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

tC-CE Phosphoramidite

Glen Research

Please Note: These products are offered under license from ISIS Pharmaceuticals, Inc. and in collaboration with ModyBase HB.

Absorption and emisssion data for tC and tC^o arecollected below:

tC	QY	l	e(L/mol.cm)
single-stranded	0.21	385nm
double-stranded	0.19
tC^o	QY	l	e(L/mol.cm)
single-stranded	0.30	360nm	9000
double-stranded	0.21

(QY determined relative to quinine sulfate in 0.5M H2SO4)

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

tC-CE Phosphoramidite

Glen Research

DILUTION/COUPLING DATA

The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.

ABI 392/394
Cat.No.	PackSize	Grams/Pack	0.1M Dil.(mL)	LV40	LV200	40nm	0.2µm	1µm	10µm
Cat.No.	PackSize	Grams/Pack	0.1M Dil.(mL)	Approximate Number of Additions
10-1516-95	50µmoles	.042grams	.5	3.33	2	1.25	.91	.67	.17
10-1516-90	100µmoles	.084grams	1	20	12	7.5	5.45	4	1
10-1516-02	0.25grams	.25grams	2.99	86.33	51.8	32.38	23.55	17.27	4.32
Expedite
Cat.No.	PackSize	Grams/Pack	Dilution(mL)	Molarity	50nm	0.2µm	1µm	15µm
Cat.No.	PackSize	Grams/Pack	Dilution(mL)	Approximate Number of Additions
10-1516-95	50µmoles	.042grams	.75	.07	8.6	5.38	3.91	.54
10-1516-90	100µmoles	.084grams	1.5	.07	23.6	14.75	10.73	1.48
10-1516-02	0.25grams	.25grams	4.46	.07	82.8	51.75	37.64	5.18
Beckman
Cat.No.	PackSize	Grams/Pack	Dilution(mL)	Molarity	30nm	200nm	1000nm
Cat.No.	PackSize	Grams/Pack	Dilution(mL)	Approximate Number of Additions
10-1516-95	50µmoles	.042grams	.75	.07	10.2	6.38	4.64
10-1516-90	100µmoles	.084grams	1.5	.07	25.2	15.75	11.45
10-1516-02	0.25grams	.25grams	4.46	.07	84.4	52.75	38.36

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

品牌介绍

Athens Research & Technology—供应多款蛋白、酶制品 Athens Research & Technology是成立于2010年的新兴生物高科技制品公司，该公司拥有BSL-2实验室及超过11000平方英尺的cGMP生产厂房，并且通过ISO 9001：2008认证。Athens公司致力于纯化分离高纯度，高活性的人类蛋白质及研发多克隆抗血清产品。提供包括高纯度及活性的丝氨酸蛋白酶，蛋白酶抑制剂，中性粒细胞酶，载脂蛋白，脂蛋白，血小板蛋白，转铁蛋白，免疫球蛋白等等。公司产品适用于炎症，冠状动脉疾病，自身免疫性疾病，癌症，阿尔茨海默氏病等众多研究领域，已被世界*制药公司及诊断试剂公司用于体外诊断试剂盒/免疫检测试剂盒、药物筛选、细胞培养液（包括干细胞）等产品研发。除了人类蛋白质，我们还从动物血清和组织中分离多种蛋白质及酶类。此外，Athens Research and Technology还提供特殊试剂/蛋白定制服务，以满足研究人员的不同需求。

公司介绍

品牌分类

转铁蛋白和apotranferrins Lab Reagents: Smart Buffers and Reagents 分子生物学用酶：分子生物学用酶蛋白质纯化：阿波罗20毫升超滤免疫球蛋白 qPCR：基因分析和基因面板 PCR : PCR Products 牛奶和血小板蛋白 DNA & RNA Sequencing : Dye Terminator clean-up

Aptamers: Catalogue Aptamers Peptide Synthesis : Amino Acids 运输和其他蛋白质 DNA: Whole DNA STEM学习：STEM工具包低内毒素水平蛋白质提取和纯化-RNA：组织/培养转染-EZ生物系统：细胞系凝血因子分析试剂盒抗体 Gel Electrophoresis: Gel Electrophoresis 中性粒细胞蛋白 Transfection - Biontex: Proteofection 体外毒理学：致突变性试剂盒寡糖合成：CEPs 动物蛋白 Extraction & Purification - DNA: Plasmid 人类蛋白质染色体绘制：人类脂蛋白和载脂蛋白样本拭子：鼻咽拭子酶和抑制剂细胞培养污染控制：检测 Antibodies: Collagenase

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Oligo Synthesis : CEPs

Prices quoted are for single packs only. For multiples of the same product please request a quote. Some of Glen"sproductsarehazardousandmay be subject to additional shipping charges. Full product information is available onGlen Research"s website.

tC-CE Phosphoramidite

Glen Research

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DILUTION/COUPLING DATA