

Oligo Synthesis : CEPs
Prices quoted are for single packs only. For multiples of the same product please request a quote. Some of Glen"sproductsarehazardousandmay be subject to additional shipping charges. Full product information is available onGlen Research"s website.
TAMRA-dT
TAMRA-dT
Glen Research
Catalogue No. | Description | Pack Size | Price | Qty |
|
---|---|---|---|---|---|
10-1057-02 | TAMRA-dT | 0.25g | £780.00£741.00Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
10-1057-90 | TAMRA-dT | 100µmoles | £396.00£376.20Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
10-1057-95 | TAMRA-dT | 50µmoles | £200.00£190.00Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
Related products
TAMRA-dT
TAMRA-dT
Glen Research
TAMRA-dT |
Catalog Number: 10-1057-xx
Description: TAMRA-dT
5"-Dimethoxytrityloxy-5-[N-((tetramethylrhodaminyl)-aminohexyl)-3-acrylimido]-2"-deoxyUridine-3"-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite | ||
Formula: C73H83N8O13P | M.W.: 1311.48 | F.W.: 870.85 |
Diluent: 10% THF in Anhydrous Acetonitrile |
Coupling: Dissolve this product in anhydrous acetonitrile:THF (9:1). Allow 15 minutes for the product to go into solution.A 6 minute coupling time is recommended. Monomers that allow for UltraMILD deprotection must be used. (Catalog Numbers: dA: 10-1601-xx, dC: 10-1015-xx, dG: 10-1621-xx, dT: 10-1030-xx.). To avoid any exchange of the iPr-Pac group on the dG with acetyl, use the UltraMild Cap Mix A (40-4210-xx/ 40-4212-xx) |
Deprotection: UltraMILD deprotection: 0.05M Potassium Carbonate in Methanol, 4hr at Room Temperature. |
Storage: Freezer storage, -10 to -30°C, dry |
Stability in Solution: 24 hours |
RHODAMINE LABELLING
Rhodamine derivatives are not sufficiently stable to survive conventional deprotection and these must be attached to amino-modified oligonucleotides using post-synthesis labelling techniques. Because Tetramethyl Rhodamine (TAMRA) is not base stable, the procedure to cleave and deprotect the labelled oligonucleotide must be carefully considered. Using the UltraMILD monomers and deprotection with potassium carbonate in methanol, TAMRA oligonucleotides can be fairly conveniently isolated. To streamline the preparation of TAMRA oligos, we offer 3’-TAMRA CPG for 3’ labelling and TAMRA-dT for labelling within the sequence. We also offer TAMRA NHS ester for labelling amino-modified oligonucleotides.
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
TAMRA-dT
TAMRA-dT
Glen Research
Material Safety Data Sheet
Glen Report 11.1: NEW FLUORESCENT REAGENTS - TAMRA-dT, DABCYL-dT |
Glen Report 21.2: Deprotection - Volume 3 - Dye-Containing Oligonucleotides |
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
TAMRA-dT
TAMRA-dT
Glen Research
5"-Dimethoxytrityloxy-5-[N-((tetramethylrhodaminyl)-aminohexyl)-3-acrylimido]-2"-deoxyUridine-3"-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite | ||
Formula: C73H83N8O13P | M.W.: 1311.48 | F.W.: 870.85 |
Diluent: 10% THF in Anhydrous Acetonitrile |
Coupling: Dissolve this product in anhydrous acetonitrile:THF (9:1). Allow 15 minutes for the product to go into solution.A 6 minute coupling time is recommended. Monomers that allow for UltraMILD deprotection must be used. (Catalog Numbers: dA: 10-1601-xx, dC: 10-1015-xx, dG: 10-1621-xx, dT: 10-1030-xx.). To avoid any exchange of the iPr-Pac group on the dG with acetyl, use the UltraMild Cap Mix A (40-4210-xx/ 40-4212-xx) |
Deprotection: UltraMILD deprotection: 0.05M Potassium Carbonate in Methanol, 4hr at Room Temperature. |
Storage: Freezer storage, -10 to -30°C, dry |
Stability in Solution: 24 hours |
Material Safety Data Sheet |
Literature Highlights
Glen Report 11.1: NEW FLUORESCENT REAGENTS - TAMRA-dT, DABCYL-dT |
Glen Report 21.2: Deprotection - Volume 3 - Dye-Containing Oligonucleotides |
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.
ABI 392/394 | |||||||||
Cat.No. | PackSize | Grams/Pack | 0.1M Dil.(mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
Approximate Number of Additions | |||||||||
10-1057-02 | 0.25grams | .25grams | 1.91 | 50.33 | 30.2 | 18.88 | 13.73 | 10.07 | 2.52 |
10-1057-90 | 100µmoles | .131grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
10-1057-95 | 50µmoles | .066grams | .5 | 3.33 | 2 | 1.25 | .91 | .67 | .17 |
Expedite | |||||||||
Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
Approximate Number of Additions | |||||||||
10-1057-02 | 0.25grams | .25grams | 2.85 | .067 | 50.6 | 31.63 | 23 | 3.16 | |
10-1057-90 | 100µmoles | .131grams | 1.5 | .067 | 23.6 | 14.75 | 10.73 | 1.48 | |
10-1057-95 | 50µmoles | .066grams | .75 | .067 | 8.6 | 5.38 | 3.91 | .54 | |
Beckman | |||||||||
Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 30nm | 200nm | 1000nm | ||
Approximate Number of Additions | |||||||||
10-1057-02 | 0.25grams | .25grams | 2.85 | .067 | 52.2 | 32.63 | 23.73 | ||
10-1057-90 | 100µmoles | .131grams | 1.5 | .067 | 25.2 | 15.75 | 11.45 | ||
10-1057-95 | 50µmoles | .066grams | .75 | .067 | 10.2 | 6.38 | 4.64 |
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
TAMRA-dT
TAMRA-dT
Glen Research
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link formore detailed usage informationwith the various synthesizers.
ABI 392/394 | |||||||||
Cat.No. | PackSize | Grams/Pack | 0.1M Dil.(mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
Approximate Number of Additions | |||||||||
10-1057-02 | 0.25grams | .25grams | 1.91 | 50.33 | 30.2 | 18.88 | 13.73 | 10.07 | 2.52 |
10-1057-90 | 100µmoles | .131grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
10-1057-95 | 50µmoles | .066grams | .5 | 3.33 | 2 | 1.25 | .91 | .67 | .17 |
Expedite | |||||||||
Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
Approximate Number of Additions | |||||||||
10-1057-02 | 0.25grams | .25grams | 2.85 | .07 | 50.6 | 31.63 | 23 | 3.16 | |
10-1057-90 | 100µmoles | .131grams | 1.5 | .07 | 23.6 | 14.75 | 10.73 | 1.48 | |
10-1057-95 | 50µmoles | .066grams | .75 | .07 | 8.6 | 5.38 | 3.91 | .54 | |
Beckman | |||||||||
Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 30nm | 200nm | 1000nm | ||
Approximate Number of Additions | |||||||||
10-1057-02 | 0.25grams | .25grams | 2.85 | .07 | 52.2 | 32.63 | 23.73 | ||
10-1057-90 | 100µmoles | .131grams | 1.5 | .07 | 25.2 | 15.75 | 11.45 | ||
10-1057-95 | 50µmoles | .066grams | .75 | .07 | 10.2 | 6.38 | 4.64 |
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200