Oligo Synthesis : CEPs
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Symmetric Doubler Phosphoramidite
Symmetric Doubler Phosphoramidite
Glen Research
| Catalogue No. | Description | Pack Size | Price | Qty |
|
|---|---|---|---|---|---|
| 10-1920-02 | Symmetric Doubler Phosphoramidite | 0.25g | £192.00£182.40Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 10-1920-90 | Symmetric Doubler Phosphoramidite | 100µmoles | £120.00£114.00Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
Related products
Symmetric Doubler Phosphoramidite
Symmetric Doubler Phosphoramidite
Glen Research
| Symmetric Doubler Phosphoramidite |
Catalog Number: 10-1920-xx
Description: Symmetric Doubler Phosphoramidite
| 1,3-bis-[5-(4,4"-dimethoxytrityloxy)pentylamido]propyl-2-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite | ||
| Formula: C64H79N4O10P | M.W.: 1095.32 | F.W.: 351.31 |
Diluent: Anhydrous Acetonitrile |
| Coupling: Synthesis should be carried out on 1000? support. A 15 minute coupling time is recommended for the doubler addition. |
| Deprotection: No changes needed from standard method recommended by synthesizer manufacturer. |
| Storage: Refrigerated storage, maximum of 2-8°C, dry |
| Stability in Solution: 2-3 days |
| Please Note: This product is supplied under license from ISIS Innovation Limited. |
DENDRIMERS
Dendrimers are discrete, highly branched, monodispersed polymers that possess patterns reminiscent of the branching of trees. Plain and mixed oligonucleotide dendrimers can be synthesized using novel doubling and trebling phosphoramidite synthons.1,2 Dendrimers offer the following advantages. Incorporation of label usingγ-32P-ATP and polynucleotide kinase increases in proportion to the number of 5"-ends. Fluorescent signal also increases in proportion to the number of 5"-ends, if spacers are incorporated between the labels and the ends of the branches. When using a dendrimeric oligonucleotide as a PCR primer, the strand bearing the dendrimer is resistant to degradation by T7 Gene 6 exonuclease making it easy to convert the double-stranded product of the PCR to a multiply labelled, single-stranded probe. Enhanced stability of DNA dendrimers makes them useful as building blocks for the ‘bottom up" approach to nano-assembly. These features also suggest applications in DNA chip technology when higher temperatures are required, for example, to melt secondary structure in the target.
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Symmetric Doubler Phosphoramidite
Symmetric Doubler Phosphoramidite
Glen Research
Material Safety Data Sheet
Glen Report 12.1: OLIGONUCLEOTIDE DENDRIMERS: FROM POLY-LABELLED DNA PROBES TO STABLE NANO-STRUCTURES
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Symmetric Doubler Phosphoramidite
Symmetric Doubler Phosphoramidite
Glen Research
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link formore detailed usage informationwith the various synthesizers.
| ABI 392/394 | |||||||||
| Cat.No. | PackSize | Grams/Pack | 0.1M Dil.(mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 10-1920-02 | 0.25grams | .25grams | 2.28 | 62.67 | 37.6 | 23.5 | 17.09 | 12.53 | 3.13 |
| 10-1920-90 | 100µmoles | .11grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| Expedite | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 10-1920-02 | 0.25grams | .25grams | 3.41 | .07 | 61.8 | 38.63 | 28.09 | 3.86 | |
| 10-1920-90 | 100µmoles | .11grams | 1.5 | .07 | 23.6 | 14.75 | 10.73 | 1.48 | |
| Beckman | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 10-1920-02 | 0.25grams | .25grams | 3.41 | .07 | 63.4 | 39.63 | 28.82 | ||
| 10-1920-90 | 100µmoles | .11grams | 1.5 | .07 | 25.2 | 15.75 | 11.45 | ||
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