Oligo Synthesis : CEPs
Prices quoted are for single packs only. For multiples of the same product please request a quote. Some of Glen"sproductsarehazardousandmay be subject to additional shipping charges. Full product information is available onGlen Research"s website.
Solid Chemical Phosphorylation Reagent II
Solid Chemical Phosphorylation Reagent II
Glen Research
| Catalogue No. | Description | Pack Size | Price | Qty |
|
|---|---|---|---|---|---|
| 10-1902-02 | Solid Chemical Phosphorylation Reagent II | 0.25g | £160.00£152.00Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 10-1902-90 | Solid Chemical Phosphorylation Reagent II | 100µmoles | £48.00£45.60Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
Related products
Solid Chemical Phosphorylation Reagent II
Solid Chemical Phosphorylation Reagent II
Glen Research
Solid Chemical Phosphorylation Reagent II |
Catalog Number: 10-1902-xx
Description: Solid Chemical Phosphorylation Reagent II
| [3-(4,4"-Dimethoxytrityloxy)-2,2-dicarboxymethylamido]propyl-(2-cyanoethyl)-(N,N-diisopropyl)-phosphoramidite | ||
| Formula: C37H49N4O7P | M.W.: 692.79 | F.W.: 79.98 |
Diluent: Anhydrous Acetonitrile |
| Coupling: No changes needed from standard method recommended by synthesizer manufacturer. |
| Deprotection: If the final DMT is removed during synthesis, deprotection under standard conditions will eliminate the Solid CPR II to yield a 5" Phosphate. If the DMT is retained, see Technical Bulletin for removal procedure (Technical Bulletin). |
| Storage: Refrigerated storage, maximum of 2-8°C, dry |
| Stability in Solution: No data available |
| Please Note: Chemical Phosphorylation Reagent II is covered by US Patent No.: 5,959,090 and European Patent: EP0816368. |
CHEMICAL PHOSPHORYLATION
Chemical Phosphorylation Reagent is most commonly used to phosphorylate the 5"-terminus of an oligonucleotide. Although this product is also successful in 3"-phosphorylation, 3"-Phosphate CPG allows direct preparation of oligonucleotides with a 3"-phosphate group. Chemical Phosphorylation Reagent II contains a DMT group on a side chain which is stable to base cleavage and can be left on the oligonucleotide for use in RP purification. The DMT group is later removed with aqueous acid and the side chain is eliminated after brief treatment with aqueous ammonium hydroxide to yield the 5"-phosphate.1 Solid CPR II is similar in performance to CPR II but it is easier to prepare aliquots since it is a powder. Many researchers treat synthesis supports with a hindered base (e.g., diethylamine, diisopropylethylamine, or DBU) post-synthesis to eliminate and remove the cyanoethyl phosphate groups. In this way, the acrylonitrile formed in situ is removed from the support and is not available to alkylate dT residues at the N3 position in the oligos. Since the sulfonylethyl group in 3"-Phosphate CPG is also susceptible to ß-elimination leading to oligo cleavage, this technique is not compatible with 3"-phosphate CPG. Using CPR II CPG, which is base labile but does not support ß-elimination, the cyanoethyl groups can be removed from the oligo prior to cleavage and base deprotection. ABI-style vials and columns are supplied unless otherwise requested.
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
Solid Chemical Phosphorylation Reagent II
Solid Chemical Phosphorylation Reagent II
Glen Research
Material Safety Data Sheet
Glen Report 20.1: New Products
Glen Report 23.1: Technical Brief - Chemical Phosphorylation ? considering the options
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
Solid Chemical Phosphorylation Reagent II
Solid Chemical Phosphorylation Reagent II
Glen Research
Chemical Phosphorylation Reagent is provided under license from Siemens Corporation.Chemical Phosphorylation Reagent II is covered by US Patent No.: 5,959,090.
(1) A. Guzaev, H.Salo, A. Azhayev, and H. Lonnberg, Tetrahedron, 1995, 51, 9375-9384.
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
Solid Chemical Phosphorylation Reagent II
Solid Chemical Phosphorylation Reagent II
Glen Research
Solid Chemical Phosphorylation Reagent II
| [3-(4,4"-Dimethoxytrityloxy)-2,2-dicarboxymethylamido]propyl-(2-cyanoethyl)-(N,N-diisopropyl)-phosphoramidite | ||
| Formula: C37H49N4O7P | M.W.: 692.79 | F.W.: 79.98 |
Diluent: Anhydrous Acetonitrile |
| Coupling: No changes needed from standard method recommended by synthesizer manufacturer. |
| Deprotection: If the final DMT is removed during synthesis, deprotection under standard conditions will eliminate the Solid CPR II to yield a 5" Phosphate. If the DMT is retained, see technical bulletin for removal procedure. Technical Bulletin |
| Storage: Refrigerated storage, maximum of 2-8°C, dry |
| Stability in Solution: No data available |
| Please Note: This product is covered by US Patent No.: 5,959,090. |
| Material Safety Data Sheet |
Literature Highlights
| Glen Report 20.1: New Products |
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.
| ABI 392/394 | |||||||||
| Cat.No. | PackSize | Grams/Pack | 0.1M Dil.(mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 10-1902-90 | 100µmoles | .069grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| 10-1902-02 | 0.25grams | .25grams | 3.61 | 107 | 64.2 | 40.13 | 29.18 | 21.4 | 5.35 |
| Expedite | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 10-1902-90 | 100µmoles | .069grams | 1.5 | .067 | 23.6 | 14.75 | 10.73 | 1.48 | |
| 10-1902-02 | 0.25grams | .25grams | 5.39 | .067 | 101.4 | 63.38 | 46.09 | 6.34 | |
| Beckman | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 10-1902-90 | 100µmoles | .069grams | 1.5 | .067 | 25.2 | 15.75 | 11.45 | ||
| 10-1902-02 | 0.25grams | .25grams | 5.39 | .067 | 103 | 64.38 | 46.82 | ||
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
Solid Chemical Phosphorylation Reagent II
Solid Chemical Phosphorylation Reagent II
Glen Research
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link formore detailed usage informationwith the various synthesizers.
| ABI 392/394 | |||||||||
| Cat.No. | PackSize | Grams/Pack | 0.1M Dil.(mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 10-1902-90 | 100µmoles | .069grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| 10-1902-02 | 0.25grams | .25grams | 3.61 | 107 | 64.2 | 40.13 | 29.18 | 21.4 | 5.35 |
| Expedite | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 10-1902-90 | 100µmoles | .069grams | 1.5 | .07 | 23.6 | 14.75 | 10.73 | 1.48 | |
| 10-1902-02 | 0.25grams | .25grams | 5.39 | .07 | 101.4 | 63.38 | 46.09 | 6.34 | |
| Beckman | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 10-1902-90 | 100µmoles | .069grams | 1.5 | .07 | 25.2 | 15.75 | 11.45 | ||
| 10-1902-02 | 0.25grams | .25grams | 5.39 | .07 | 103 | 64.38 | 46.82 | ||
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
