Oligo Synthesis : CEPs
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Pyrrolo-dC-CE Phosphoramidite
Pyrrolo-dC-CE Phosphoramidite
Glen Research
| Catalogue No. | Description | Pack Size | Price | Qty |
|
|---|---|---|---|---|---|
| 10-1017-02 | Pyrrolo-dC-CE Phosphoramidite | 0.25g | £540.00£513.00Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 10-1017-90 | Pyrrolo-dC-CE Phosphoramidite | 100µmoles | £176.00£167.20Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 10-1017-95 | Pyrrolo-dC-CE Phosphoramidite | 50µmoles | £88.00£83.60Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
Related products
Pyrrolo-dC-CE Phosphoramidite
Pyrrolo-dC-CE Phosphoramidite
Glen Research
| Pyrrolo-dC-CE Phosphoramidite |
Catalog Number: 10-1017-xx
Description: Pyrrolo-dC-CE Phosphoramidite
| 5"-Dimethoxytrityl-[6-methyl-pyrrolo-[2,3-d]-pyrimidine-2(3H)-one]-2"-deoxyribonucleoside, 3"-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite | ||
| Formula: C42H50N5O7P | M.W.: 767.85 | F.W.: 327.23 |
Diluent: Anhydrous Acetonitrile |
| Coupling: Standard coupling time. Use 0.02 M Iodine for Oxidation. |
| Deprotection: Ammonium Hydroxide for 24 hours at room temperature. |
| Storage: Refrigerated storage, maximum of 2-8°C, dry |
| Stability in Solution: 2-3 days |
| Please Note: This product is covered by US Patent No.: 7,144,995. Patented under European patent number 1483280. |
FLUORESCENT NUCLEOSIDES
Etheno-dA is a fluorescent nucleoside which is especially useful in observing the transition between DNA structural types. It is quite base labile and should be deprotected with ammonium hydroxide at room temperature for 24 hours. Alternatively, UltraMild chemistry can be used. 2-Aminopurine and AP-dC (G-Clamp) are also useful fluorescent nucleosides.
Pyrrolo-dC is a fluorescent deoxycytidine analog that is an ideal probe of DNA structure and dynamics. It base-pairs as a normal dC nucleotide. An oligo fully substituted with pyrrolo-dC has the same ™ as the control dC oligo with the same specificity for dG. Its small size does not perturb the structure of the DNA helix and it is well tolerated by a number of DNA and RNA polymerases. It is highly fluorescent and its excitation and emission are well to the red of most fluorescent nucleotide analogs, which eliminates or reduces background fluorescence from proteins. Pyrrolo-dCTP has potential uses in biological assay development.
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Pyrrolo-dC-CE Phosphoramidite
Pyrrolo-dC-CE Phosphoramidite
Glen Research
Material Safety Data Sheet
Glen Report 15.1: Pyrrolo-C - a novel fluorescent nucleoside
Glen Report 16.1: Minor Base and Related Novel Phosphoramidites
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Pyrrolo-dC-CE Phosphoramidite
Pyrrolo-dC-CE Phosphoramidite
Glen Research
EXTINCTION DATA
| Item | Nucleoside | λMax-1 | Emax-1 | λMax-2 | Emax-2 | E260 |
| (nm) | (ml/µmole) | nm | (ml/µmole) | (ml/µmole) | ||
| 10-1017 | Pyrrolo-dC | 339 | 2.36 | 229 | 17.5 | 2.41 |
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link formore detailed usage informationwith the various synthesizers.
| ABI 392/394 | |||||||||
| Cat.No. | PackSize | Grams/Pack | 0.1M Dil.(mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 10-1017-02 | 0.25grams | .25grams | 3.26 | 95.33 | 57.2 | 35.75 | 26 | 19.07 | 4.77 |
| 10-1017-90 | 100µmoles | .077grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| 10-1017-95 | 50µmoles | .038grams | .5 | 3.33 | 2 | 1.25 | .91 | .67 | .17 |
| Expedite | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 10-1017-02 | 0.25grams | .25grams | 4.86 | .07 | 90.8 | 56.75 | 41.27 | 5.68 | |
| 10-1017-90 | 100µmoles | .077grams | 1.5 | .07 | 23.6 | 14.75 | 10.73 | 1.48 | |
| 10-1017-95 | 50µmoles | .038grams | .75 | .07 | 8.6 | 5.38 | 3.91 | .54 | |
| Beckman | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 10-1017-02 | 0.25grams | .25grams | 4.86 | .07 | 92.4 | 57.75 | 42 | ||
| 10-1017-90 | 100µmoles | .077grams | 1.5 | .07 | 25.2 | 15.75 | 11.45 | ||
| 10-1017-95 | 50µmoles | .038grams | .75 | .07 | 10.2 | 6.38 | 4.64 | ||
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