Oligo Synthesis : CEPs
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Pyrrolidine-CE Phosphoramidite
Pyrrolidine-CE Phosphoramidite
Glen Research
| Catalogue No. | Description | Pack Size | Price | Qty |
|
|---|---|---|---|---|---|
| 10-1915-02 | Pyrrolidine-CE Phosphoramidite | 0.25g | £868.00£824.60Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 10-1915-90 | Pyrrolidine-CE Phosphoramidite | 100µmoles | £304.00£288.80Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 10-1915-95 | Pyrrolidine-CE Phosphoramidite | 50µmoles | £152.00£144.40Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
Pyrrolidine-CE Phosphoramidite
Pyrrolidine-CE Phosphoramidite
Glen Research
| Pyrrolidine-CE Phosphoramidite |
Catalog Number: 10-1915-xx
Description: Pyrrolidine-CE Phosphoramidite
| (2R, 3S)-2-Dimethoxytrityloxymethyl-N-(fluorenylmethyl)-pyrrolidin-3-yl-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite | ||
| Formula: C50H56N3O7P | M.W.: 841.97 | F.W.: 178.10 |
Diluent: Anhydrous Acetonitrile |
| Coupling: A 6 minute coupling time is recommended. |
| Deprotection: No changes needed from standard method recommended by synthesizer manufacturer. |
| Storage: Freezer storage, -10 to -30°C, dry |
| Stability in Solution: 2-3 days |
Base excision repair (BER) is one of the most studied repair mechanisms. In this pathway, DNA glycosylases recognize the damaged bases and catalyze their excision through hydrolysis of the N-glycosidic bond. Attempts to understand the structural basis for DNA damage recognition by DNA glycosylases have been hampered by the short-lived association of these enzymes with their DNA substrates. To overcome this problem, the Verdine group at Harvard synthesized a pyrrolidine analog that mimics the charged transition state of the enzyme-substrate complex. When incorporated into double-stranded DNA, they found the pyrrolidine analog (PYR), introduced as the Pyrrolidine-CE Phosphoramidite, forms an extremely stable complex with the DNA glycosylase AlkA, exhibiting a dissociation constant in the pM range and potently inhibited the reaction catalyzed by the enzyme
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Pyrrolidine-CE Phosphoramidite
Pyrrolidine-CE Phosphoramidite
Glen Research
Material Safety Data Sheet
Glen Report 19.2: Pyrrolidine CE Phosphoramidite: a new base excision repair inhibitor |
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
Pyrrolidine-CE Phosphoramidite
Pyrrolidine-CE Phosphoramidite
Glen Research
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link formore detailed usage informationwith the various synthesizers.
| ABI 392/394 | |||||||||
| Cat.No. | PackSize | Grams/Pack | 0.1M Dil.(mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 10-1915-90 | 100µmoles | .084grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| 10-1915-95 | 50µmoles | .042grams | .5 | 3.33 | 2 | 1.25 | .91 | .67 | .17 |
| 10-1915-02 | 0.25grams | .25grams | 2.97 | 85.67 | 51.4 | 32.13 | 23.36 | 17.13 | 4.28 |
| Expedite | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 10-1915-90 | 100µmoles | .084grams | 1.5 | .07 | 23.6 | 14.75 | 10.73 | 1.48 | |
| 10-1915-95 | 50µmoles | .042grams | .75 | .07 | 8.6 | 5.38 | 3.91 | .54 | |
| 10-1915-02 | 0.25grams | .25grams | 4.43 | .07 | 82.2 | 51.38 | 37.36 | 5.14 | |
| Beckman | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 10-1915-90 | 100µmoles | .084grams | 1.5 | .07 | 25.2 | 15.75 | 11.45 | ||
| 10-1915-95 | 50µmoles | .042grams | .75 | .07 | 10.2 | 6.38 | 4.64 | ||
| 10-1915-02 | 0.25grams | .25grams | 4.43 | .07 | 83.8 | 52.38 | 38.09 | ||
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