

Oligo Synthesis : CEPs
Prices quoted are for single packs only. For multiples of the same product please request a quote. Some of Glen"sproductsarehazardousandmay be subject to additional shipping charges. Full product information is available onGlen Research"s website.
Pyrrolidine-CE Phosphoramidite
Pyrrolidine-CE Phosphoramidite
Glen Research
Catalogue No. | Description | Pack Size | Price | Qty |
|
---|---|---|---|---|---|
10-1915-02 | Pyrrolidine-CE Phosphoramidite | 0.25g | £868.00£824.60Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
10-1915-90 | Pyrrolidine-CE Phosphoramidite | 100µmoles | £304.00£288.80Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
10-1915-95 | Pyrrolidine-CE Phosphoramidite | 50µmoles | £152.00£144.40Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
Pyrrolidine-CE Phosphoramidite
Pyrrolidine-CE Phosphoramidite
Glen Research
Pyrrolidine-CE Phosphoramidite |
Catalog Number: 10-1915-xx
Description: Pyrrolidine-CE Phosphoramidite
(2R, 3S)-2-Dimethoxytrityloxymethyl-N-(fluorenylmethyl)-pyrrolidin-3-yl-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite | ||
Formula: C50H56N3O7P | M.W.: 841.97 | F.W.: 178.10 |
Diluent: Anhydrous Acetonitrile |
Coupling: A 6 minute coupling time is recommended. |
Deprotection: No changes needed from standard method recommended by synthesizer manufacturer. |
Storage: Freezer storage, -10 to -30°C, dry |
Stability in Solution: 2-3 days |
Base excision repair (BER) is one of the most studied repair mechanisms. In this pathway, DNA glycosylases recognize the damaged bases and catalyze their excision through hydrolysis of the N-glycosidic bond. Attempts to understand the structural basis for DNA damage recognition by DNA glycosylases have been hampered by the short-lived association of these enzymes with their DNA substrates. To overcome this problem, the Verdine group at Harvard synthesized a pyrrolidine analog that mimics the charged transition state of the enzyme-substrate complex. When incorporated into double-stranded DNA, they found the pyrrolidine analog (PYR), introduced as the Pyrrolidine-CE Phosphoramidite, forms an extremely stable complex with the DNA glycosylase AlkA, exhibiting a dissociation constant in the pM range and potently inhibited the reaction catalyzed by the enzyme
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Pyrrolidine-CE Phosphoramidite
Pyrrolidine-CE Phosphoramidite
Glen Research
Material Safety Data Sheet
Glen Report 19.2: Pyrrolidine CE Phosphoramidite: a new base excision repair inhibitor |
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
Pyrrolidine-CE Phosphoramidite
Pyrrolidine-CE Phosphoramidite
Glen Research
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link formore detailed usage informationwith the various synthesizers.
ABI 392/394 | |||||||||
Cat.No. | PackSize | Grams/Pack | 0.1M Dil.(mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
Approximate Number of Additions | |||||||||
10-1915-90 | 100µmoles | .084grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
10-1915-95 | 50µmoles | .042grams | .5 | 3.33 | 2 | 1.25 | .91 | .67 | .17 |
10-1915-02 | 0.25grams | .25grams | 2.97 | 85.67 | 51.4 | 32.13 | 23.36 | 17.13 | 4.28 |
Expedite | |||||||||
Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
Approximate Number of Additions | |||||||||
10-1915-90 | 100µmoles | .084grams | 1.5 | .07 | 23.6 | 14.75 | 10.73 | 1.48 | |
10-1915-95 | 50µmoles | .042grams | .75 | .07 | 8.6 | 5.38 | 3.91 | .54 | |
10-1915-02 | 0.25grams | .25grams | 4.43 | .07 | 82.2 | 51.38 | 37.36 | 5.14 | |
Beckman | |||||||||
Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 30nm | 200nm | 1000nm | ||
Approximate Number of Additions | |||||||||
10-1915-90 | 100µmoles | .084grams | 1.5 | .07 | 25.2 | 15.75 | 11.45 | ||
10-1915-95 | 50µmoles | .042grams | .75 | .07 | 10.2 | 6.38 | 4.64 | ||
10-1915-02 | 0.25grams | .25grams | 4.43 | .07 | 83.8 | 52.38 | 38.09 |
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200