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商品详细Athens Research/Cambio - Excellence in Molecular Biology/0.25g/10-1590-02
Athens Research/Cambio - Excellence in Molecular Biology/0.25g/10-1590-02
Athens Research/Cambio - Excellence in Molecular Biology/0.25g/10-1590-02
商品编号: 10-1590-02
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商品介绍
Oligo Synthesis

Oligo Synthesis : CEPs

Prices quoted are for single packs only. For multiples of the same product please request a quote. Some of Glen"sproductsarehazardousandmay be subject to additional shipping charges. Full product information is available onGlen Research"s website.

  • Catalogue
  • Description
  • Protocols
  • Notes
  • Applications & Benefits

Pyrene-dU-CE Phosphoramidite

Pyrene-dU-CE Phosphoramidite

Glen Research

Catalogue No.DescriptionPack SizePriceQty
  • Change to:
  • €
10-1590-02Pyrene-dU-CE Phosphoramidite0.25g£452.00£418.00Offer until : 31-Mar-2021Offer Code : GLEN55% off all Glen productsView OfferQuantityAdd to Order
10-1590-90Pyrene-dU-CE Phosphoramidite100µmoles£188.00£159.60Offer until : 31-Mar-2021Offer Code : GLEN55% off all Glen productsView OfferQuantityAdd to Order
10-1590-95Pyrene-dU-CE Phosphoramidite50µmoles£102.00£79.80Offer until : 31-Mar-2021Offer Code : GLEN55% off all Glen productsView OfferQuantityAdd to Order

Pyrene-dU-CE Phosphoramidite

Pyrene-dU-CE Phosphoramidite

Glen Research

Pyrene-dU-CE Phosphoramidite

Structure

Catalog Number: 10-1590-xx

Description: Pyrene-dU-CE Phosphoramidite

5"-Dimethoxytrityl-5-(pyren-1-yl-ethynyl)-2"-deoxyUridine,3"-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
Formula: C57H55N4O8PM.W.: 955.04F.W.: 514.42

Diluent: Anhydrous Acetonitrile/Dichloromethane 1:1 (v/v)
Coupling: 6 minute coupling time recommended.
Deprotection: No changes needed from standard method recommended by synthesizer manufacturer.
Storage: Freezer storage, -10 to -30°C, dry
Stability in Solution: 1-2days

FLUORESCENT NUCLEOSIDES

Etheno-dA is a fluorescent nucleoside which is especially useful in observing the transition between DNA structural types. It is quite base labile and should be deprotected with ammonium hydroxide at room temperature for 24 hours. Alternatively, UltraMild chemistry can be used. 2-Aminopurine and AP-dC (G-Clamp) are also useful fluorescent nucleosides.

Pyrrolo-dC is a fluorescent deoxycytidine analog that is an ideal probe of DNA structure and dynamics. It base-pairs as a normal dC nucleotide. An oligo fully substituted with pyrrolo-dC has the same ™ as the control dC oligo with the same specificity for dG. Its small size does not perturb the structure of the DNA helix and it is well tolerated by a number of DNA and RNA polymerases. It is highly fluorescent and its excitation and emission are well to the red of most fluorescent nucleotide analogs, which eliminates or reduces background fluorescence from proteins. Pyrrolo-dCTP has potential uses in biological assay development.

When a benzylcarbamoyl analogue of AP-dC (G-Clamp) was synthesized, it was found that, when incorporated into an oligo, it exhibited similar fluorescence to AP-dC. However, when base-paired against the 8-oxo-dG, its fluorescence was severely quenched. Rather remarkably, however, when base paired with dG or any of the other bases, A, C or T, there was no change in fluorescence – making it a specific probe for 8-oxo-dG.

By attaching pyrene or perylene to the 5 position of deoxyuridine through a triple bond, the fluorophore is electronically coupled to the deoxyuridine base. This electronic coupling of the base and the fluorophore makes the fluorescence sensitive to the base pairing of the dU portion of the molecule, allowing the discrimination between perfect and one base mismatched targets.

The base analogue tCnitrois a FRET-acceptor together with tCO (or tC) as the donor molecule. This constitutes the first ever description of a nucleobase FRET-pair. This novel FRET-pair provides a unique tool for investigations of nucleic acid containing systems. tCnitrois virtually non-fluorescent under normal conditions.

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

Pyrene-dU-CE Phosphoramidite

Pyrene-dU-CE Phosphoramidite

Glen Research

Material Safety Data Sheet

Glen Report 19.2: Versatile New Reagents ? Pyrene-dU and Perylene-dU

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

Pyrene-dU-CE Phosphoramidite

Pyrene-dU-CE Phosphoramidite

Glen Research

LITERATURE HIGHLIGHTS

Glen Report 19.2: Versatile New Reagents ? Pyrene-dU and Perylene-dU

REQUENTLY ASKED TECHNICAL QUESTION

QUESTION: What are the relative extinction coefficients of 5"-Fluorescein, Hex and Tet etc.. at 260 nm and their Lambda max?

RESPONSE:Please seehttp://www.glenresearch.com/Technical/Extinctions.html

REFERENCE(S):Oligonucleotide Properties Calculator;http://www.basic.northwestern.edu/biotools/oligocalc.html

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

Pyrene-dU-CE Phosphoramidite

Pyrene-dU-CE Phosphoramidite

Glen Research

DILUTION/COUPLING DATA

The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link formore detailed usage informationwith the various synthesizers.

ABI 392/394
Cat.No.PackSizeGrams/Pack0.1M Dil.(mL)LV40LV20040nm0.2µm1µm10µm
Approximate Number of Additions
10-1590-9550µmoles.048grams.53.3321.25.91.67.17
10-1590-90100µmoles.096grams120127.55.4541
10-1590-020.25grams.25grams2.627444.427.7520.1814.83.7
Expedite
Cat.No.PackSizeGrams/PackDilution(mL)Molarity50nm0.2µm1µm15µm
Approximate Number of Additions
10-1590-9550µmoles.048grams.75.078.65.383.91.54
10-1590-90100µmoles.096grams1.5.0723.614.7510.731.48
10-1590-020.25grams.25grams3.91.0771.844.8832.644.49
Beckman
Cat.No.PackSizeGrams/PackDilution(mL)Molarity30nm200nm1000nm
Approximate Number of Additions
10-1590-9550µmoles.048grams.75.0710.26.384.64
10-1590-90100µmoles.096grams1.5.0725.215.7511.45
10-1590-020.25grams.25grams3.91.0773.445.8833.36

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

品牌介绍
Athens Research & Technology—供应多款蛋白、酶制品 Athens Research & Technology是成立于2010年的新兴生物高科技制品公司,该公司拥有BSL-2实验室及超过11000平方英尺的cGMP生产厂房,并且通过ISO 9001:2008认证。Athens公司致力于纯化分离高纯度,高活性的人类蛋白质及研发多克隆抗血清产品。提供包括高纯度及活性的丝氨酸蛋白酶,蛋白酶抑制剂,中性粒细胞酶,载脂蛋白,脂蛋白,血小板蛋白,转铁蛋白,免疫球蛋白等等。公司产品适用于炎症,冠状动脉疾病,自身免疫性疾病,癌症,阿尔茨海默氏病等众多研究领域,已被世界*制药公司及诊断试剂公司用于体外诊断试剂盒/免疫检测试剂盒、药物筛选、细胞培养液(包括干细胞)等产品研发。除了人类蛋白质,我们还从动物血清和组织中分离多种蛋白质及酶类。此外,Athens Research and Technology还提供特殊试剂/蛋白定制服务,以满足研究人员的不同需求。