Oligo Synthesis : CEPs
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Perylene-dU-CE Phosphoramidite
Perylene-dU-CE Phosphoramidite
Glen Research
| Catalogue No. | Description | Pack Size | Price | Qty |
|
|---|---|---|---|---|---|
| 10-1591-02 | Perylene-dU-CE Phosphoramidite | 0.25g | £600.00£547.20Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 10-1591-90 | Perylene-dU-CE Phosphoramidite | 100µmoles | £256.00£228.00Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 10-1591-95 | Perylene-dU-CE Phosphoramidite | 50µmoles | £136.00£114.00Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
Related products
Perylene-dU-CE Phosphoramidite
Perylene-dU-CE Phosphoramidite
Glen Research
| Perylene-dU-CE Phosphoramidite |
Catalog Number: 10-1591-xx
Description: Perylene-dU-CE Phosphoramidite
| 5"-Dimethoxytrityl-5-(perylen-3-yl-ethynyl)-2"-deoxyUridine,3"-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite | ||
| Formula: C61H57N4O8P | M.W.: 1005.10 | F.W.: 564.48 |
Diluent: Anhydrous Acetonitrile/Dichloromethane 1:1 (v/v) |
| Coupling: A 6 minute coupling time is recommended. |
| Deprotection: No changes needed from standard method recommended by synthesizer manufacturer. |
| Storage: Refrigerated storage, maximum of 2-8°C, dry |
| Stability in Solution: 1-2days |
When a benzylcarbamoyl analogue of AP-dC (G-Clamp) was synthesized, it was found that, when incorporated into an oligo, it exhibited similar fluorescence to AP-dC. However, when base-paired against the 8-oxo-dG, its fluorescence was severely quenched. Rather remarkably, however, when base paired with dG or any of the other bases, A, C or T, there was no change in fluorescence – making it a specific probe for 8-oxo-dG.
By attaching pyrene or perylene to the 5 position of deoxyuridine through a triple bond, the fluorophore is electronically coupled to the deoxyuridine base. This electronic coupling of the base and the fluorophore makes the fluorescence sensitive to the base pairing of the dU portion of the molecule, allowing the discrimination between perfect and one base mismatched targets.
The base analogue tCnitrois a FRET-acceptor together with tCO (or tC) as the donor molecule. This constitutes the first ever description of a nucleobase FRET-pair. This novel FRET-pair provides a unique tool for investigations of nucleic acid containing systems. tCnitrois virtually non-fluorescent under normal conditions.
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Perylene-dU-CE Phosphoramidite
Perylene-dU-CE Phosphoramidite
Glen Research
Material Safety Data Sheet
Glen Report 19.2: Versatile New Reagents ? Pyrene-dU and Perylene-dU
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Perylene-dU-CE Phosphoramidite
Perylene-dU-CE Phosphoramidite
Glen Research
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link formore detailed usage informationwith the various synthesizers.
| ABI 392/394 | |||||||||
| Cat.No. | PackSize | Grams/Pack | 0.1M Dil.(mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 10-1591-95 | 50µmoles | .05grams | .5 | 3.33 | 2 | 1.25 | .91 | .67 | .17 |
| 10-1591-90 | 100µmoles | .101grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| 10-1591-02 | 0.25grams | .25grams | 2.49 | 69.67 | 41.8 | 26.13 | 19 | 13.93 | 3.48 |
| Expedite | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 10-1591-95 | 50µmoles | .05grams | .75 | .07 | 8.6 | 5.38 | 3.91 | .54 | |
| 10-1591-90 | 100µmoles | .101grams | 1.5 | .07 | 23.6 | 14.75 | 10.73 | 1.48 | |
| 10-1591-02 | 0.25grams | .25grams | 3.71 | .07 | 67.8 | 42.38 | 30.82 | 4.24 | |
| Beckman | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 10-1591-95 | 50µmoles | .05grams | .75 | .07 | 10.2 | 6.38 | 4.64 | ||
| 10-1591-90 | 100µmoles | .101grams | 1.5 | .07 | 25.2 | 15.75 | 11.45 | ||
| 10-1591-02 | 0.25grams | .25grams | 3.71 | .07 | 69.4 | 43.38 | 31.55 | ||
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