Oligo Synthesis : CEPs
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pdC-CE Phosphoramidite
pdC-CE Phosphoramidite
Glen Research
| Catalogue No. | Description | Pack Size | Price | Qty |
|
|---|---|---|---|---|---|
| 10-1014-02 | pdC-CE Phosphoramidite | 0.25g | £196.00£186.20Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 10-1014-05 | pdC-CE Phosphoramidite | 0.5g | £392.00£372.40Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 10-1014-90 | pdC-CE Phosphoramidite | 100µmoles | £88.00£64.60Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
Related products
pdC-CE Phosphoramidite
pdC-CE Phosphoramidite
Glen Research
| pdC-CE Phosphoramidite |
Catalog Number: 10-1014-xx
Description: pdC-CE Phosphoramidite
| 5"-Dimethoxytrityl-N4-diisobutylaminomethylidene-5-(1-Propynyl)-2"-deoxyCytidine,3"-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite | ||
| Formula: C51H67N6O7P | M.W.: 907.1 | F.W.: 327.23 |
Diluent: Anhydrous Acetonitrile |
| Coupling: No changes needed from standard method recommended by synthesizer manufacturer. |
| Deprotection: Deprotection in Ammonium Hydroxide requires a minimum of 12 hours at 55 °C. |
| Storage: Refrigerated storage, maximum of 2-8°C, dry |
| Stability in Solution: Similar to dA,C,G,T-CE Phosphoramidites |
| Please Note: This product is covered by patents or patents pending owned by Isis Pharmaceuticals, Inc. ("Isis"). Purchase of this product includes a limited license to use this product solely for internal research. This license specifically excludes (and you have no right to use this product for): (a) therapeutic or diagnostic applications (including products or services that incorporate this product), (b) any in vivo toxicity/safety study in support of an investigational new drug application (or foreign counterpart), (c) resale (including sale of any products or services that incorporate this product) or (d) gene functionalization activities (including products or services that incorporate data derived from gene functionalization activities) if such activities have commercial application, any and all of which require a separate license from Isis. Neither this product nor any product created through its use may be used in human clinical trials. |
| Please see Terms and Conditions of Use |
C-5 PROPYNE DERIVATIVES AND G-CLAMP
Substitution of C-5 propynyl-dC (pdC) for dC and C-5 propynyl-dU (pdU) for dT are effective strategies to enhance base pairing. Using these base substitutions, duplex stability and melting temperatures are raised by the following amounts: C-5 propynyl-C 2.8° per substitution; C-5 propynyl-U 1.7° per substitution. AP-dC (G-clamp) substitutes for dC and is another very important modified nucleoside that enhances hybridization by 7.5° per substitution. The ability of these modified bases to enhance binding while maintaining specificity has proven useful in antisense research and in the synthesis of high affinity probes. AP-dC is also a fluorescent nucleoside and should find uses in DNA structural research.
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pdC-CE Phosphoramidite
pdC-CE Phosphoramidite
Glen Research
Material Safety Data Sheet
Glen Report 9.1: A NOVEL TRANSFECTION REAGENT
Glen Report 6.2: C-5 PROPYNE DERIVATIVES - ANTISENSE BREAKTHROUGH?
Glen Report 10.1: CYTOFECTIN GSV TRANSFECTION PROTOCOL
Glen Report 11.1: USING MODIFIED BASES TO OPTIMIZE HYBRIDIZATION
Glen Report 13.1: C-5 PROPYNES, CYTOFECTIN AND OTHER PRODUCT UPDATES
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pdC-CE Phosphoramidite
pdC-CE Phosphoramidite
Glen Research
C-5 Propyne and AP-dC Phosphoramidites
This product is covered by patents or patents pending owned by Isis Pharmaceuticals, Inc. (Isis). Purchase of this product includes a limited licence to use this product solely for internal research. This licence specifically excludes (an you have no right to use this product for): (a) therapeutic or diagnostic applications (including products or services that incorporate this product), (b) any in vivo toxicity/safety study in support of an investigational new drug application (or foreigh counterpart), (c) resale (including sale of any products or services that incorporate this product), or (d) gene functionalisation activities (including products or services that incorporate data derived from gene functionalisation activities) if such activities have commercial application, any and all of which require a separate licence from Isis. Neither this product nor any product created through its use may be used in human clinical trials.
A simple agreement must be signed before end-users and custom oligo services amy purchase these products for use as defined above.
FREQUENTLY ASKED TECHNICAL QUESTION
QUESTION: What are the extinction coefficients for propynyl-dC and dU? Also Halogenated dC and dU derivatives? Others?
RESPONSE:See:Table of Extinction Coefficients
REFERENCE(S):B. Froehler, GileadM. PowellR. Somers
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pdC-CE Phosphoramidite
pdC-CE Phosphoramidite
Glen Research
EXTINCTION DATA
| Item | Nucleoside | λMax-1 | Emax-1 | λMax-2 | Emax-2 | E260 |
| (nm) | (ml/µmole) | nm | (ml/µmole) | (ml/µmole) | ||
| 10-1014 | pdC | 295 | 7.7 | 234 | 14.7 | 5.1 |
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link formore detailed usage informationwith the various synthesizers.
| ABI 392/394 | |||||||||
| Cat.No. | PackSize | Grams/Pack | 0.1M Dil.(mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 10-1014-02 | 0.25grams | .25grams | 2.76 | 78.67 | 47.2 | 29.5 | 21.45 | 15.73 | 3.93 |
| 10-1014-05 | 0.5grams | .5grams | 5.51 | 170.33 | 102.2 | 63.88 | 46.45 | 34.07 | 8.52 |
| 10-1014-90 | 100µmoles | .091grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| Expedite | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 10-1014-02 | 0.25grams | .25grams | 4.11 | .07 | 75.8 | 47.38 | 34.45 | 4.74 | |
| 10-1014-05 | 0.5grams | .5grams | 8.23 | .07 | 158.2 | 98.88 | 71.91 | 9.89 | |
| 10-1014-90 | 100µmoles | .091grams | 1.5 | .07 | 23.6 | 14.75 | 10.73 | 1.48 | |
| Beckman | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 10-1014-02 | 0.25grams | .25grams | 4.11 | .07 | 77.4 | 48.38 | 35.18 | ||
| 10-1014-05 | 0.5grams | .5grams | 8.23 | .07 | 159.8 | 99.88 | 72.64 | ||
| 10-1014-90 | 100µmoles | .091grams | 1.5 | .07 | 25.2 | 15.75 | 11.45 | ||
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