Oligo Synthesis : CEPs
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PC Spacer Phosphoramidite
PC Spacer Phosphoramidite
Glen Research
| Catalogue No. | Description | Pack Size | Price | Qty |
|
|---|---|---|---|---|---|
| 10-4913-02 | PC Spacer Phosphoramidite | 0.25g | £316.00£300.20Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 10-4913-90 | PC Spacer Phosphoramidite | 100µmoles | £108.00£102.60Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
Related products
PC Spacer Phosphoramidite
PC Spacer Phosphoramidite
Glen Research
| PC Spacer Phosphoramidite |
Catalog Number: 10-4913-xx
Description: PC Spacer Phosphoramidite
| [4-(4,4"-Dimethoxytrityloxy)butyramidomethyl)-1-(2-nitrophenyl)-ethyl]-2-cyanoethyl-(N,N-diisopropyl)-phosphoramidite | ||
| Formula: C43H53N4O8P | M.W.: 784.88 | F.W.: 344.26 |
Diluent: Anhydrous Acetonitrile |
| Coupling: 2 minute coupling time recommended |
| Deprotection: No changes needed from standard method recommended by synthesizer manufacturer.Technical Bulletin |
| Storage: Freezer storage, -10 to -30°C, dry |
| Stability in Solution: 2-3 days |
| Please Note: Glen Research offers PC Biotin, PC Amino-Modifier and PC Spacer products in association with AmberGen, Inc. and Link Technologies, Ltd. For a commercial application license, please contact AmberGen, Inc.,617-975-0680, http://www.ambergen.com/. |
ALDEHYDE MODIFICATION
Aldehyde modifiers would be attractive electrophilic substitutions in oligonucleotides since they are able to react with amino groups to form a Schiff"s base, with hydrazino groups to form hydrazones, and with semicarbazides to form semi-carbazones. The Schiff"s base is unstable and must be reduced with sodium borohydride to form a stable linkage but hydrazones and semicarbazides are very stable linkages.
Our collaboration with Epoch Biosciences, a subsidiary of Nanogen, Inc., has allowed us to offer 5"-Aldehyde-Modifier C2 Phosphoramidite. The acetal protecting group is sufficiently hydrophobic for use in RP HPLC and cartridge purification and is readily removed after oligonucleotide synthesis under standard oligonucleotide detritylation conditions with 80% acetic acid / 20% water or 2% aqueous trifluoroacetic acid during cartridge purification.
A formylindole nucleoside analogue has been used to introduce aldehyde groups within an oligonucleotide or at the 5" terminus. This product has no protecting group on the aldehyde, which means that deprotection of the modified oligonucleotide can be done without changing preferred conditions.
SPACER MODIFIERS
The spacer phosphoramidites C3, 9, C12 and 18 are used to insert a spacer arm in an oligonucleotide. The compounds may be added in multiple additions when a longer spacer is required. 3’-Spacer C3 CPG may also act as a blocker of exonuclease and polymerase activity at the 3’-terminus. dSpacer is used to introduce a stable abasic site within an oligonucleotide. PC Spacer is a photocleavable C3 spacer modifier, part of our line of photocleavable (PC) modifiers
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PC Spacer Phosphoramidite
PC Spacer Phosphoramidite
Glen Research
Material Safety Data Sheet
| Glen Report 14.1: PC Biotin and Related Photocleavable Modifiers |
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
PC Spacer Phosphoramidite
PC Spacer Phosphoramidite
Glen Research
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link formore detailed usage informationwith the various synthesizers.
| ABI 392/394 | |||||||||
| Cat.No. | PackSize | Grams/Pack | 0.1M Dil.(mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 10-4913-02 | 0.25grams | .25grams | 3.19 | 93 | 55.8 | 34.88 | 25.36 | 18.6 | 4.65 |
| 10-4913-90 | 100µmoles | .078grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| Expedite | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 10-4913-02 | 0.25grams | .25grams | 4.75 | .07 | 88.6 | 55.38 | 40.27 | 5.54 | |
| 10-4913-90 | 100µmoles | .078grams | 1.5 | .07 | 23.6 | 14.75 | 10.73 | 1.48 | |
| Beckman | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 10-4913-02 | 0.25grams | .25grams | 4.75 | .07 | 90.2 | 56.38 | 41 | ||
| 10-4913-90 | 100µmoles | .078grams | 1.5 | .07 | 25.2 | 15.75 | 11.45 | ||
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
