Oligo Synthesis : CEPs
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PC Linker Phosphoramidite
PC Linker Phosphoramidite
Glen Research
| Catalogue No. | Description | Pack Size | Price | Qty |
|
|---|---|---|---|---|---|
| 10-4920-02 | PC Linker Phosphoramidite | 0.25g | £636.00£604.20Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 10-4920-90 | PC Linker Phosphoramidite | 100µmoles | £204.00£193.80Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
Related products
PC Linker Phosphoramidite
PC Linker Phosphoramidite
Glen Research
| PC Linker Phosphoramidite |
Catalog Number: 10-4950-xx
Description: PC Biotin Phosphoramidite
| 1-[2-Nitro-5-(6-(N-(4,4"-dimethoxytrityl))-biotinamidocaproamidomethyl)phenyl]-ethyl-[2-cyanoethyl-(N,N-diisopropyl)]-phosphoramidite | ||
| Formula: C55H72N7O9PS | M.W.: 1038.25 | F.W.: 597.62 |
Diluent: Anhydrous Acetonitrile |
| Coupling: 2 minute coupling time recommended |
| Deprotection: PC-Biotin is slow to detritylate. If the final DMT-group is to be removed on the synthesizer, we recommend a second deblocking step.Technical Bulletin |
| Storage: Freezer storage, -10 to -30°C, dry |
| Stability in Solution: 2-3 days |
| Please Note: Glen Research offers PC Biotin, PC Amino-Modifier and PC Spacer products in association with AmberGen, Inc. and Link Technologies, Ltd. For a commercial application license, please contact AmberGen, Inc.,617-975-0680, http://www.ambergen.com/. |
PHOTOCLEAVABLE MONOMERS
PC Biotin Phosphoramidite can be used to prepare 5’-biotinylated oligonucleotides suitable for capture by streptavidin in a mode similar to our popular 5’ Biotin Phosphoramidite. Amino- and thiol-modified oligonucleotides have proven to be very useful for the attachment of a variety of haptens and fluorophores, as well as for the tethering of the oligonucleotides to a diversity of beads and surfaces. PC Amino-Modifier Phosphoramidite is used to prepare 5’-amino-modified oligonucleotides suitable for subsequent photocleavage. PC Spacer Phosphoramidite can be used as an intermediary to attach any modification reagent, available as a phosphoramidite, to the terminus of oligonucleotides. After photocleavage, a 5’-phosphate is generated on the DNA, rendering it suitable for further biological transformations, such as gene construction and cloning after ligation.
A versatile photocleavable DNA building block has been described by researchers in Washington University, Missouri and used in phototriggered hybridization.1This reagent has also been used in the design of multifunctional DNA and RNA conjugates2for the in vitro selection of new molecules catalyzing biomolecular reactions. Researchers at Bruker Daltonik in Germany have also developed genoSNIP, a method for single-nucleotide polymorphism (SNP) genotyping by MALDI-TOF mass spectrometry.3This method uses size reduction of primer extension products by incorporation of the photocleavable linker for phototriggering strand breaks near to the 3" end of the extension primer. PC Linker can be incorporated into oligonucleotides at any position by standard automated DNA synthesis methodology. PC Linker Phosphoramidite has the added advantage in that photocleavage results in monophosphate fragments at both the 3"- and 5"-termini of the oligonucleotide fragments.
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PC Linker Phosphoramidite
PC Linker Phosphoramidite
Glen Research
Material Safety Data Sheet
| Glen Report 14.1: PC Biotin and Related Photocleavable Modifiers |
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
PC Linker Phosphoramidite
PC Linker Phosphoramidite
Glen Research
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link formore detailed usage informationwith the various synthesizers.
| ABI 392/394 | |||||||||
| Cat.No. | PackSize | Grams/Pack | 0.1M Dil.(mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 10-4950-02 | 0.25grams | .25grams | 2.41 | 67 | 40.2 | 25.13 | 18.27 | 13.4 | 3.35 |
| 10-4950-90 | 100µmoles | .104grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| 10-4950-95 | 50µmoles | .052grams | .5 | 3.33 | 2 | 1.25 | .91 | .67 | .17 |
| Expedite | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 10-4950-02 | 0.25grams | .25grams | 3.59 | .07 | 65.4 | 40.88 | 29.73 | 4.09 | |
| 10-4950-90 | 100µmoles | .104grams | 1.5 | .07 | 23.6 | 14.75 | 10.73 | 1.48 | |
| 10-4950-95 | 50µmoles | .052grams | .75 | .07 | 8.6 | 5.38 | 3.91 | .54 | |
| Beckman | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 10-4950-02 | 0.25grams | .25grams | 3.59 | .07 | 67 | 41.88 | 30.45 | ||
| 10-4950-90 | 100µmoles | .104grams | 1.5 | .07 | 25.2 | 15.75 | 11.45 | ||
| 10-4950-95 | 50µmoles | .052grams | .75 | .07 | 10.2 | 6.38 | 4.64 | ||
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