Oligo Synthesis : CEPs
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O6-Me-dG-CE Phosphoramidite
O6-Me-dG-CE Phosphoramidite
Glen Research
| Catalogue No. | Description | Pack Size | Price | Qty |
|
|---|---|---|---|---|---|
| 10-1070-02 | O6-Me-dG-CE Phosphoramidite | 0.25g | £204.00£193.80Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 10-1070-90 | O6-Me-dG-CE Phosphoramidite | 100µmoles | £84.00£79.80Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
Related products
O6-Me-dG-CE Phosphoramidite
O6-Me-dG-CE Phosphoramidite
Glen Research
| O6-Me-dG-CE Phosphoramidite |
Catalog Number: 10-1070-xx
Description: O6-Me-dG-CE Phosphoramidite
| 5"-Dimethoxytrityl-N-isobutyryl-O6-methyl-2"-deoxyGuanosine,3"-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite | ||
| Formula: C45H56N7O8P | M.W.: 853.97 | F.W.: 343.24 |
Diluent: Anhydrous Acetonitrile |
| Coupling: No changes needed from standard method recommended by synthesizer manufacturer. |
| Deprotection: Treat the CPG with 10% DBU in anhydrous methanol at Room Temperature for 5 days in the dark to cleave and deprotect the oligo.1) Prepare a solution of 10% DBU in anhydrous methanol. DBU (1,8-Diazabicyclo[5.4.0]undec-7-ene) is available from Aldrich (139009-25G) as is anhydrous methanol (322415-100ML).2) Transfer the CPG to a clean, dry glass vial and add 1 mL of the 10% DBU solution.3) Seal the vial and leave for 5 days in the dark at room temperature.4) Transfer the contents to a micro centrifuge tube and reduce under vacuum to a small volume.5) Add 1mL of 10mM aqueous sodium hydroxide solution and desalt or purify the oligonucleotide using standard procedures.Technical Bulletin |
| Storage: Refrigerated storage, maximum of 2-8°C, dry |
| Stability in Solution: 2-3 days |
MUTAGENESIS
Cellular polynucleotides are alkylated by endogenous components, such as S-adenosylmethionine, or after reacting with two general classes of environmental and laboratory chemicals. SN1 chemical agents include alkylnitrosourea and N-alkyl-N-nitro-N-nitrosoguanidine that react with the N7 position of guanine, N3 of adenine, O6 of guanine, O2 or O4 of pyrimidines, and the non-phosphodiester oxygen atoms of the phosphate backbone. In contrast, SN2 chemical agents such as methyl methanesulfonate and dimethyl sulfate react primarily with the N1 position of adenine (1-Methyl-2’-deoxyadenosine) and N3 of cytosine. To avoid chain branching during synthesis when using DCI as activator, N6-Me-dA is offered with acetyl protection.
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O6-Me-dG-CE Phosphoramidite
O6-Me-dG-CE Phosphoramidite
Glen Research
Material Safety Data Sheet
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
O6-Me-dG-CE Phosphoramidite
O6-Me-dG-CE Phosphoramidite
Glen Research
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link formore detailed usage informationwith the various synthesizers.
| ABI 392/394 | |||||||||
| Cat.No. | PackSize | Grams/Pack | 0.1M Dil.(mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 10-1070-02 | 0.25grams | .25grams | 2.93 | 84.33 | 50.6 | 31.63 | 23 | 16.87 | 4.22 |
| 10-1070-90 | 100µmoles | .085grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| Expedite | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 10-1070-02 | 0.25grams | .25grams | 4.37 | .07 | 81 | 50.63 | 36.82 | 5.06 | |
| 10-1070-90 | 100µmoles | .085grams | 1.5 | .07 | 23.6 | 14.75 | 10.73 | 1.48 | |
| Beckman | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 10-1070-02 | 0.25grams | .25grams | 4.37 | .07 | 82.6 | 51.63 | 37.55 | ||
| 10-1070-90 | 100µmoles | .085grams | 1.5 | .07 | 25.2 | 15.75 | 11.45 | ||
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
