Oligo Synthesis : CEPs
Prices quoted are for single packs only. For multiples of the same product please request a quote. Some of Glen"sproductsarehazardousandmay be subject to additional shipping charges. Full product information is available onGlen Research"s website.
N-POM Caged-dT-CE Phosphoramidite
N-POM Caged-dT-CE Phosphoramidite
Glen Research
| Catalogue No. | Description | Pack Size | Price | Qty |
|
|---|---|---|---|---|---|
| 10-1534-02 | N-POM Caged-dT-CE Phosphoramidite | 0.25g | £716.00£680.20Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 10-1534-90 | N-POM Caged-dT-CE Phosphoramidite | 100 µmole | £284.00£269.80Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 10-1534-95 | N-POM Caged-dT-CE Phosphoramidite | 50 µmole | £148.00£140.60Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
N-POM Caged-dT-CE Phosphoramidite
N-POM Caged-dT-CE Phosphoramidite
Glen Research
N-POM Caged-dT-CE Phosphoramidite
Catalog Number: 10-1534-xx
Description: N-POM Caged-dT-CE Phosphoramidite
| 5"-Dimethoxytrityl-N3-[[1-(6-nitro-1,3-benzodioxol-5-yl)ethoxy]methyl]-2"-deoxyThymidine,3"-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite | ||
| Formula: C50H58N5O13P | M.W.: 967.99 | F.W.: 527.38 (N-POM-dT) FW: 304.20 (dT) |
Diluent: Anhydrous Acetonitrile |
| Coupling: No changes needed from standard method recommended by synthesizer manufacturer. |
| Deprotection: No changes needed from standard method recommended by synthesizer manufacturer. |
| Storage: Refrigerated storage, maximum of 2-8°C, dry. Light sensitive material. |
| Stability in Solution: 1-2 days |
CAGED NUCLEOSIDES
Glen Research’s interest lies in the preparation of caged oligonucleotides whose function is restored after uncaging by UV light at a wavelength that causes no DNA damage. The Deiters group at North Carolina State University has described NPOM-Caged-dT, where the nucleobase is caged with the photolabile group, 6-nitropiperonyloxymethyl (NPOM), which can be removed using UV light at 365nm. Oligonucleotides containing NPOM-Caged-dT every five or six bases do not hybridize to their complementary strand. Photo-uncaging of the caged oligonucleotide is then easily carried out with UV light at 365 nm for seconds to minutes to restore the activity of the oligonucleotide.
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N-POM Caged-dT-CE Phosphoramidite
N-POM Caged-dT-CE Phosphoramidite
Glen Research
Material Safety Data Sheet
| Glen Report 23.2: New Product - NPOM-Caged-dT |
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
N-POM Caged-dT-CE Phosphoramidite
N-POM Caged-dT-CE Phosphoramidite
Glen Research
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link formore detailed usage informationwith the various synthesizers.
| ABI 392/394 | |||||||||
| Cat.No. | PackSize | Grams/Pack | 0.1M Dil.(mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 10-1534-02 | 0.25grams | .25grams | 2.58 | 72.67 | 43.6 | 27.25 | 19.82 | 14.53 | 3.63 |
| 10-1534-90 | 100µmoles | .097grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| 10-1534-95 | 50µmoles | .048grams | .5 | 3.33 | 2 | 1.25 | .91 | .67 | .17 |
| Expedite | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 10-1534-02 | 0.25grams | .25grams | 3.85 | .07 | 70.6 | 44.13 | 32.09 | 4.41 | |
| 10-1534-90 | 100µmoles | .097grams | 1.5 | .07 | 23.6 | 14.75 | 10.73 | 1.48 | |
| 10-1534-95 | 50µmoles | .048grams | .75 | .07 | 8.6 | 5.38 | 3.91 | .54 | |
| Beckman | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 10-1534-02 | 0.25grams | .25grams | 3.85 | .07 | 72.2 | 45.13 | 32.82 | ||
| 10-1534-90 | 100µmoles | .097grams | 1.5 | .07 | 25.2 | 15.75 | 11.45 | ||
| 10-1534-95 | 50µmoles | .048grams | .75 | .07 | 10.2 | 6.38 | 4.64 | ||
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
