Oligo Synthesis : CEPs
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Long Trebler Phosphoramidite
Long Trebler Phosphoramidite
Glen Research
| Catalogue No. | Description | Pack Size | Price | Qty |
|
|---|---|---|---|---|---|
| 10-1925-02 | Long Trebler Phosphoramidite | 0.25g | £240.00£228.00Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 10-1925-90 | Long Trebler Phosphoramidite | 100µmoles | £160.00£152.00Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
Related products
Long Trebler Phosphoramidite
Long Trebler Phosphoramidite
Glen Research
| Long Trebler Phosphoramidite |
Catalog Number: 10-1925-xx
Description: Long Trebler Phosphoramidite
| Tris-2,2,2-[3-(4,4"-dimethoxytrityloxy)propyloxymethyl]methyleneoxypropyl-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite | ||
| Formula: C89H107N2O15P | M.W.: 1475.78 | F.W.: 428.41 |
Diluent: Anhydrous AcetonitrileAdd fresh diluent to product vial to recommended concentration and swirl vial occasionally over several minutes until product is completely dissolved. (Some oils may require between 5 and 10 minutes.) Use care to maintain anhydrous conditions. In case of transfer to alternate vial type, ensure recipient vial has been pre-dried. For more information, see http://www.glenres.com/ProductFiles/Technical/TB_ABITransfer.pdf. |
| Coupling: Synthesis should be carried out on 1000 support. A 15 minute coupling time using DCI activator (30-3150-xx) is recommended for the trebler addition. |
| Deprotection: No changes needed from standard method recommended by synthesizer manufacturer. |
| Storage: Freezer storage, -10 to -30°C, dry |
| Stability in Solution: 1-2 days |
Dendrimers
Dendrimers are discrete, highly branched, monodispersed polymers that possess patterns reminiscent of the branching of trees. Plain and mixed oligonucleotide dendrimers can be synthesized using novel doubling and trebling phosphoramidite synthons.1,2 Dendrimers offer the following advantages. Incorporation of label using γ-32P-ATP and polynucleotide kinase increases in proportion to the number of 5’-ends. Fluorescent signal also increases in proportion to the number of 5’-ends, if spacers are incorporated between the labels and the ends of the branches. When using a dendrimeric oligonucleotide as a PCR primer, the strand bearing the dendrimer is resistant to degradation by T7 Gene 6 exonuclease making it easy to convert the double-stranded product of the PCR to a multiply labelled, single-stranded probe. Enhanced stability of DNA dendrimers makes them useful as building blocks for the ‘bottom up’ approach to nano-assembly. These features also suggest applications in DNA chip technology when higher temperatures are required, for example, to melt secondary structure in the target.
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Long Trebler Phosphoramidite
Long Trebler Phosphoramidite
Glen Research
Material Safety Data Sheet
Glen Report 19.1: Microarrays, Nanotechnology and Beyond
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Long Trebler Phosphoramidite
Long Trebler Phosphoramidite
Glen Research
(1) M.S. Shchepinov, I.A. Udalova, A.J. Bridgman, and E.M. Southern, Nucleic Acids Res, 1997, 25, 4447-4454.
(2) M.S. Shchepinov, K.U. Mir, J.K. Elder, M.D. Frank-Kamenetskii, and E.M. Southern, Nucleic Acids Res, 1999, 27, 3035-41.
(3) T. Horn, C.A. Chang, and M.S. Urdea, Nucleic Acids Res, 1997, 25, 4842-4849.
Doublers and Trebler are supplied under license from ISIS Innovation Limited.
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Long Trebler Phosphoramidite
Long Trebler Phosphoramidite
Glen Research
Long Trebler Phosphoramidite
| Tris-2,2,2-[3-(4,4"-dimethoxytrityloxy)propyloxymethyl]methyleneoxypropyl-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite | ||
| Formula: C89H107N2O15P | M.W.: 1475.78 | F.W.: 428.41 |
Diluent: Anhydrous Acetonitrile |
| Coupling: Synthesis should be carried out on 1000 support. A 15 minute coupling time using DCI activator (30-3150-xx) is recommended for the trebler addition. |
| Deprotection: No changes needed from standard method recommended by synthesizer manufacturer. |
| Storage: Freezer storage, -10 to -30°C, dry |
| Stability in Solution: 1-2 days |
| Material Safety Data Sheet |
Literature Highlights
| Glen Report 19.1: Microarrays, Nanotechnology and Beyond |
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.
| ABI 392/394 | |||||||||
| Cat.No. | PackSize | Grams/Pack | 0.1M Dil.(mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 10-1925-90 | 100µmoles | .148grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| 10-1925-02 | 0.25grams | .25grams | 1.69 | 43 | 25.8 | 16.13 | 11.73 | 8.6 | 2.15 |
| Expedite | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 10-1925-90 | 100µmoles | .148grams | 1.5 | .067 | 23.6 | 14.75 | 10.73 | 1.48 | |
| 10-1925-02 | 0.25grams | .25grams | 2.53 | .067 | 44.2 | 27.63 | 20.09 | 2.76 | |
| Beckman | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 10-1925-90 | 100µmoles | .148grams | 1.5 | .067 | 25.2 | 15.75 | 11.45 | ||
| 10-1925-02 | 0.25grams | .25grams | 2.53 | .067 | 45.8 | 28.63 | 20.82 | ||
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
Long Trebler Phosphoramidite
Long Trebler Phosphoramidite
Glen Research
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link formore detailed usage informationwith the various synthesizers.
| ABI 392/394 | |||||||||
| Cat.No. | PackSize | Grams/Pack | 0.1M Dil.(mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 10-1925-90 | 100µmoles | .148grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| 10-1925-02 | 0.25grams | .25grams | 1.69 | 43 | 25.8 | 16.13 | 11.73 | 8.6 | 2.15 |
| Expedite | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 10-1925-90 | 100µmoles | .148grams | 1.5 | .07 | 23.6 | 14.75 | 10.73 | 1.48 | |
| 10-1925-02 | 0.25grams | .25grams | 2.53 | .07 | 44.2 | 27.63 | 20.09 | 2.76 | |
| Beckman | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 10-1925-90 | 100µmoles | .148grams | 1.5 | .07 | 25.2 | 15.75 | 11.45 | ||
| 10-1925-02 | 0.25grams | .25grams | 2.53 | .07 | 45.8 | 28.63 | 20.82 | ||
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
