Oligo Synthesis : CEPs
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I-CE Phosphoramidite
I-CE Phosphoramidite
Glen Research
| Catalogue No. | Description | Pack Size | Price | Qty |
|
|---|---|---|---|---|---|
| 10-3040-02 | I-CE Phosphoramidite | 0.25g | £400.00£380.00Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 10-3040-90 | I-CE Phosphoramidite | 100µmoles | £152.00£144.40Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 10-3040-95 | I-CE Phosphoramidite | 50µmoles | £76.00£72.20Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
Related products
I-CE Phosphoramidite
I-CE Phosphoramidite
Glen Research
| I-CE Phosphoramidite |
Catalog Number: 10-3040-xx
Description: I-CE Phosphoramidite
| 5"-Dimethoxytrityl-Inosine, 2"-O-TBDMS-3"-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite | ||
| Formula: C46H61N6O8PSi | M.W.: 885.08 | F.W.: 330.19 |
Diluent: Anhydrous Acetonitrile |
| Coupling: 12 minute coupling time recommended. |
| Deprotection: RNA deprotection- seeTechnical Bulletin |
| Storage: Freezer storage, -10 to -30°C, dry |
| Stability in Solution: 2-3 days |
MINOR RNA PHOSPHORAMIDITES (TBDMS PROTECTED)
Inosine and 5-Methyl-Uridine are useful for analyzing RNA structure and activity relationships. 5-Bromo-Uridine and 5-Iodo-Uridine have been used for crystallography studies and cross-linking experiments. 6-Thioguanosine (6-thio-G) has applications in ribozyme and siRNA research, as well as in RNA-protein interactions. The removal of the silyl protecting group without interfering with the sulfur is critical. This is removed1cleanly by triethylamine trihydrofluoride in DMSO but t-butylammonium fluoride (TBAF) leads to degradation of the thio-nucleotide analogue and should not be used. 2-Aminopurine riboside is useful for analyzing RNA structure and activity relationships, for example, in ribozyme studies.
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I-CE Phosphoramidite
I-CE Phosphoramidite
Glen Research
Material Safety Data Sheet
Glen Report 19.1: Modified RNA Phosphoramidites Useful In Sirna Research And Biologically Significant 1-Methy-Adenosine
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I-CE Phosphoramidite
I-CE Phosphoramidite
Glen Research
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link formore detailed usage informationwith the various synthesizers.
| ABI 392/394 | |||||||||
| Cat.No. | PackSize | Grams/Pack | 0.1M Dil.(mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 10-3040-02 | 0.25grams | .25grams | 2.82 | 80.67 | 48.4 | 30.25 | 22 | 16.13 | 4.03 |
| 10-3040-90 | 100µmoles | .089grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| 10-3040-95 | 50µmoles | .044grams | .5 | 3.33 | 2 | 1.25 | .91 | .67 | .17 |
| Expedite | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 10-3040-02 | 0.25grams | .25grams | 4.22 | .07 | N/A | N/A | 16.25 | N/A | |
| 10-3040-90 | 100µmoles | .089grams | 1.5 | .07 | N/A | N/A | 4.92 | N/A | |
| 10-3040-95 | 50µmoles | .044grams | .75 | .07 | N/A | N/A | 1.79 | N/A | |
| Beckman | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 10-3040-02 | 0.25grams | .25grams | 4.22 | .07 | N/A | 12.84 | 12.06 | ||
| 10-3040-90 | 100µmoles | .089grams | 1.5 | .07 | N/A | 4.06 | 3.82 | ||
| 10-3040-95 | 50µmoles | .044grams | .75 | .07 | N/A | 1.65 | 1.55 | ||
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
