Oligo Synthesis : CEPs
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GTT Trimer Phosphoramidite
GTT Trimer Phosphoramidite
Glen Research
| Catalogue No. | Description | Pack Size | Price | Qty |
|
|---|---|---|---|---|---|
| 13-1233-90 | GTT Trimer Phosphoramidite | 100µmoles | £680.00£646.00Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 13-1233-95 | GTT Trimer Phosphoramidite | 50µmoles | £340.00£323.00Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
Related products
GTT Trimer Phosphoramidite
GTT Trimer Phosphoramidite
Glen Research
| GTT Trimer Phosphoramidite |
Catalog Number: 13-1233-xx
Description: GTT Trimer Phosphoramidite
| 5"-Dimethoxytrityl-N2-isobutyryl-2"-deoxyGuanosine-3"->5"-o-chlorophenyl-phosphoryl-2"-deoxyThymidine-3"->5"-o-chlorophenyl-phosphoryl-2"-deoxyThymidine-3"-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite | ||
| Formula: C76H86Cl2N11O22P3 | M.W.: 1667.5 | |
Diluent: Anhydrous Acetonitrile/Dichloromethane 1:3 (v/v) |
| Coupling:15 minute coupling time recommended. |
| Deprotection: 30% NH4OH for 17 hours at room temperature followed by an additional 4 hours at 55°C. |
| Storage: Freezer storage, -10 to -30°C, dry |
| Stability in Solution: 2-3 days |
EXAMPLE OF PREPARATION OF TRIMER MIXTURE
Prepare 530 mg of the trimer mix, taking the amount (mg) for each trimer from the right column. Dissolve the trimer mix in dichloromethane (highest grade possible; acid-free). Evaporate to dryness to produce a homogenous mixture of all 20 trimers.
EXAMPLE OF PREPARATION OF TRIMER MIXTURE FOR THE SYNTHESIZER
Dissolve 530 mg, which is equivalent to 20X10 µmoles (normalized for RF) of the trimer mix in 2.0 mL of acetonitrile-dichloromethane mixture, 1:3 v/v to produce a 0.10N solution of trimers, ready for use in a synthesizer.
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
GTT Trimer Phosphoramidite
GTT Trimer Phosphoramidite
Glen Research
Material Safety Data Sheet
| Glen Report 16.2: TRIMER (CODON) PHOSPHORAMIDITES SIMPLIFY LIBRARY PREPARATION |
| Glen Report 18.1: Trimer Phosphoramidites - Tools for fine-tuning protein function |
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
GTT Trimer Phosphoramidite
GTT Trimer Phosphoramidite
Glen Research
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link formore detailed usage informationwith the various synthesizers.
| ABI 392/394 | |||||||||
| Cat.No. | PackSize | Grams/Pack | 0.1M Dil.(mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 13-1233-90 | 100µmoles | .167grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| 13-1233-95 | 50µmoles | .083grams | .5 | 3.33 | 2 | 1.25 | .91 | .67 | .17 |
| Expedite | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 13-1233-90 | 100µmoles | .167grams | 1.5 | .07 | 23.6 | 14.75 | 10.73 | 1.48 | |
| 13-1233-95 | 50µmoles | .083grams | .75 | .07 | 8.6 | 5.38 | 3.91 | .54 | |
| Beckman | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 13-1233-90 | 100µmoles | .167grams | 1.5 | .07 | 25.2 | 15.75 | 11.45 | ||
| 13-1233-95 | 50µmoles | .083grams | .75 | .07 | 10.2 | 6.38 | 4.64 | ||
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
