Oligo Synthesis : CEPs
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GAA Trimer Phosphoramidite
GAA Trimer Phosphoramidite
Glen Research
| Catalogue No. | Description | Pack Size | Price | Qty |
|
|---|---|---|---|---|---|
| 13-1200-90 | GAA Trimer Phosphoramidite | 100µmoles | £680.00£646.00Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 13-1200-95 | GAA Trimer Phosphoramidite | 50µmoles | £340.00£323.00Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
Related products
GAA Trimer Phosphoramidite
GAA Trimer Phosphoramidite
Glen Research
| GAA Trimer Phosphoramidite |
Catalog Number: 13-1200-xx
Description: GAA Trimer Phosphoramidite
| 5"-Dimethoxytrityl-N2-isobutyryl-2"-deoxyGuanosine-3"->5"-o-chlorophenyl-phosphoryl-N6-benzoyl-2"-deoxyAdenosine-3"->5"-o-chlorophenyl-phosphoryl-N6-benzoyl-2"-deoxyAdenosine-3"-(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite | ||
| Formula: C90H92Cl2N17O20P3 | M.W.: 1893.5 | |
Diluent: Anhydrous Acetonitrile/Dichloromethane 1:3 (v/v) |
| Coupling:15 minute coupling time recommended. |
| Deprotection: 30% NH4OH for 17 hours at room temperature followed by an additional 4 hours at 55°C. |
| Storage: Freezer storage, -10 to -30°C, dry |
| Stability in Solution: 2-3 days |
EXAMPLE OF PREPARATION OF TRIMER MIXTURE FOR THE SYNTHESIZER
Dissolve 530 mg, which is equivalent to 20X10 µmoles (normalized for RF) of the trimer mix in 2.0 mL of acetonitrile-dichloromethane mixture, 1:3 v/v to produce a 0.10N solution of trimers, ready for use in a synthesizer.
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
GAA Trimer Phosphoramidite
GAA Trimer Phosphoramidite
Glen Research
Material Safety Data Sheet
| Glen Report 16.2: TRIMER (CODON) PHOSPHORAMIDITES SIMPLIFY LIBRARY PREPARATION |
| Glen Report 18.1: Trimer Phosphoramidites - Tools for fine-tuning protein function |
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
GAA Trimer Phosphoramidite
GAA Trimer Phosphoramidite
Glen Research
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link formore detailed usage informationwith the various synthesizers.
| ABI 392/394 | |||||||||
| Cat.No. | PackSize | Grams/Pack | 0.1M Dil.(mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 13-1200-90 | 100µmoles | .189grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| 13-1200-95 | 50µmoles | .095grams | .5 | 3.33 | 2 | 1.25 | .91 | .67 | .17 |
| Expedite | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 13-1200-90 | 100µmoles | .189grams | 1.5 | .07 | 23.6 | 14.75 | 10.73 | 1.48 | |
| 13-1200-95 | 50µmoles | .095grams | .75 | .07 | 8.6 | 5.38 | 3.91 | .54 | |
| Beckman | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 13-1200-90 | 100µmoles | .189grams | 1.5 | .07 | 25.2 | 15.75 | 11.45 | ||
| 13-1200-95 | 50µmoles | .095grams | .75 | .07 | 10.2 | 6.38 | 4.64 | ||
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
