Oligo Synthesis : CEPs
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Ferrocene-dT-CE Phosphoramidite
Ferrocene-dT-CE Phosphoramidite
Glen Research
| Catalogue No. | Description | Pack Size | Price | Qty |
|
|---|---|---|---|---|---|
| 10-1576-02 | Ferrocene-dT-CE Phosphoramidite | 0.25g | £600.00£570.00Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 10-1576-90 | Ferrocene-dT-CE Phosphoramidite | 100umole | £280.00£266.00Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 10-1576-95 | Ferrocene-dT-CE Phosphoramidite | 50umole | £140.00£133.00Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
Ferrocene-dT-CE Phosphoramidite
Ferrocene-dT-CE Phosphoramidite
Glen Research
Catalog Number: 10-1576-xx
Description: Ferrocene-dT-CE Phosphoramidite
| 5"-dimethoxytrityloxy-5-[N-acetyl ferrocenyl-aminohexyl)-3-acrylimido]-2"-deoxyUridine-3"-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite | ||
| Formula: C60H73FeN6O10P | M.W.: 1125.07 | F.W.: 684.45 |
Diluent: Anhydrous Acetonitrile |
| Coupling: 3 minute coupling time recommended |
| Deprotection: Synthesize using acetyl-protected dC (10-1015-xx) and deprotect in 30% Ammonium Hydroxide/40% Methylamine 1:1 (AMA) at 65°C for 10 minutes OR Synthesize using UltraMild monomers and Cap A (Catalog Numbers: dA: 10-1601-xx, dC: 10-1015-xx, dG: 10-1621-xx, dT: 10-1030-xx, Cap Mix A (40-4210-xx/ 40-4212-xx) and deprotect in Ammonium Hydroxide for 2 hours at room temperature. |
| Storage: Freezer storage, -10 to -30°C, dry |
| Stability in Solution: 1-2 days |
FERROCENE LABELLING
With an excellent stability profile, ferrocene has always attracted considerable interest for DNA labelling to generate probes for electrochemical detection. Based on our Amino-Modifier C6-dT structure, Ferrocene-dT is easily added to oligonucleotides with no disruption of regular hybridization behavior. Multiple incorporations into an oligonucleotide probe are also simply achieved. Oligonucleotides are deprotected using standard techniques. Ferrocene oligonucleotides should be stored under Argon and aqueous solutions should be degassed immediately.
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Ferrocene-dT-CE Phosphoramidite
Ferrocene-dT-CE Phosphoramidite
Glen Research
Material Safety Data Sheet
http://www.glenresearch.com/GlenReports/GR22-22.html
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
Ferrocene-dT-CE Phosphoramidite
Ferrocene-dT-CE Phosphoramidite
Glen Research
- A E Navarro, et al., Nucleic Acids Res., 2004, 32, 5310-5319
- H Brisset, A E Navarro, N Spinelli, C Chaix, and B Mandrand, Biotechnology Journal, 2006, 1, 95-98
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
Ferrocene-dT-CE Phosphoramidite
Ferrocene-dT-CE Phosphoramidite
Glen Research
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link formore detailed usage informationwith the various synthesizers.
| ABI 392/394 | |||||||||
| Cat.No. | PackSize | Grams/Pack | 0.1M Dil.(mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 10-1576-02 | 0.25grams | .25grams | 2.22 | 60.67 | 36.4 | 22.75 | 16.55 | 12.13 | 3.03 |
| 10-1576-90 | 100µmoles | .113grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| 10-1576-95 | 50µmoles | .056grams | .5 | 3.33 | 2 | 1.25 | .91 | .67 | .17 |
| Expedite | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 10-1576-02 | 0.25grams | .25grams | 3.32 | .07 | 60 | 37.5 | 27.27 | 3.75 | |
| 10-1576-90 | 100µmoles | .113grams | 1.5 | .07 | 23.6 | 14.75 | 10.73 | 1.48 | |
| 10-1576-95 | 50µmoles | .056grams | .75 | .07 | 8.6 | 5.38 | 3.91 | .54 | |
| Beckman | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 10-1576-02 | 0.25grams | .25grams | 3.32 | .07 | 61.6 | 38.5 | 28 | ||
| 10-1576-90 | 100µmoles | .113grams | 1.5 | .07 | 25.2 | 15.75 | 11.45 | ||
| 10-1576-95 | 50µmoles | .056grams | .75 | .07 | 10.2 | 6.38 | 4.64 | ||
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