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商品详细雅典研究院/坎比奥-卓越分子生物学/50µ摩尔/10-5925-95
雅典研究院/坎比奥-卓越分子生物学/50µ摩尔/10-5925-95
雅典研究院/坎比奥-卓越分子生物学/50µ摩尔/10-5925-95
商品编号: 10-5925-95
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商品介绍
Oligo Synthesis

Oligo Synthesis : CEPs

Prices quoted are for single packs only. For multiples of the same product please request a quote. Some of Glen"sproductsarehazardousandmay be subject to additional shipping charges. Full product information is available onGlen Research"s website.

  • Catalogue
  • Description
  • Protocols
  • Notes

Epoch EclipseTM Quencher Phosphoramidite

Epoch EclipseTM Quencher Phosphoramidite

Glen Research

Catalogue No.DescriptionPack SizePriceQty
  • Change to:
  • €
10-5925-02Epoch EclipseTM Quencher Phosphoramidite0.25g£1,308.00£900.60Offer until : 31-Mar-2021Offer Code : GLEN55% off all Glen productsView OfferQuantityAdd to Order
10-5925-90Epoch EclipseTM Quencher Phosphoramidite100µmoles£528.00£364.80Offer until : 31-Mar-2021Offer Code : GLEN55% off all Glen productsView OfferQuantityAdd to Order
10-5925-95Epoch EclipseTM Quencher Phosphoramidite50µmoles£264.00£190.00Offer until : 31-Mar-2021Offer Code : GLEN55% off all Glen productsView OfferQuantityAdd to Order

Epoch EclipseTM Quencher Phosphoramidite

Epoch EclipseTM Quencher Phosphoramidite

Glen Research

Epoch EclipseTM Quencher Phosphoramidite

Structure

Catalog Number: 20-5925-xx

Description: Epoch Eclipse™Quencher CPG

4-N-methyl-N-(4"-nitro-2"-chloroazobenzen-4-yl)-aminobutanamido-1-(2-O-dimethoxytrityloxymethyl)-pyrrolidin-4-yl-succinoyl long chain alkylamino-CPG
F.W.: 537.89

Diluent: n/a
Coupling: This support should be used in a manner identical to normal protected nucleoside support since it contains the DMT group.
Deprotection: Deprotect using the protocol required by the nucleobases.
Storage: Freezer storage, -10 to -30°C, dry
Stability in Solution:
Please Note: This Product is for research purposes only, and may not be used for commercial, clinical, diagnostic or any other use. The Product is subject to proprietary rights of Epoch Biosciences, Inc. and is made and sold under license from Epoch Biosciences, Inc. There is no implied license for commercial use with respect to this Product and a license must be obtained directly from Epoch Biosciences, Inc. with respect to any proposed commercial use of this Product. "Commercial use" includes but is not limited to the sale, lease, license or other transfer of the Product or any material derived or produced from it, the sale, lease, license or other grant of rights to use the Product or any material derived or produced from it, or the use of the Product to perform services for a fee for third parties (including contract research). Eclipse, Gig Harbor Green, Redmond Red and Yakima Yellow are trademarks of Epoch Biosciences, Inc.

Please see Terms and Conditions of Use

EPOCH DYES AND QUENCHER

Glen Research’s agreement with Epoch Biosciences, Inc., a subsidiary of Nanogen, Inc., allows us to sell several of Epoch’s proprietary products designed for the synthesis of novel DNA probes. We are pleased to offer products based on Epoch’s Redmond Red™, Yakima Yellow™ and Gig Harbor Green™ fluorophores and Eclipse™ non-fluorescent quencher. Under our agreement we also supply PPG, a modified nucleoside and 5’-Aldehyde-Modifier C2 Phosphoramidite. The two fluorescent dyes, Yakima Yellow and Redmond Red, are available as phosphoramidites and supports. Yakima Yellow has an absorbance maximum at 530 nm and emission maximum at 549 nm, while Redmond Red’s absorbance and emission maxima are at 579 nm and 595 nm, respectively. Gig Harbor Green and 6-FAM are based on the same fluorescein core structure but Gig Harbor Green is 15-20% brighter than FAM.

The Eclipse quencher from Epoch solves most of the problems inherent in the synthesis of molecular beacon and FRET probes. The Eclipse molecule is highly stable and can be used safely in all common oligo deprotection schemes. The absorbance maximum for Eclipse Quencher is at 522 nm, compared to 479 nm for dabcyl. In addition, the structure of the Eclipse Quencher is substantially more electron deficient than that of dabcyl and this leads to better quenching over a wider range of dyes, especially those with emission maxima at longer wavelengths (red shifted) such as Redmond Red and Cy5. In addition, with an absorption range from 390 nm to 625 nm, the Eclipse Quencher is capable of effective performance in a wide range of colored FRET probes.

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

Epoch EclipseTM Quencher Phosphoramidite

Epoch EclipseTM Quencher Phosphoramidite

Glen Research

Material Safety Data Sheet

Glen Report 15.1: Redmond red (TM) And yakima yellow (TM) dyes, and eclipse (TM) non-fluorescent quencher

Glen Report 21.2: Deprotection - Volume 3 - Dye-Containing Oligonucleotides

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

Epoch EclipseTM Quencher Phosphoramidite

Epoch EclipseTM Quencher Phosphoramidite

Glen Research

FREQUENTLY ASKED TECHNICAL QUESTION

QUESTION: What are the relative extinction coefficients of 5"-Fluorescein, Hex and Tet etc.. at 260 nm and their Lambda max?

RESPONSE:Please seehttp://www.glenresearch.com/Technical/Extinctions.html

REFERENCE(S):Oligonucleotide Properties Calculator;http://www.basic.northwestern.edu/biotools/oligocalc.html


QUESTION: What are the relative extinction coefficients of various dyes?

RESPONSE:Please seehttp://www.glenres.com/Technical/Extinctions.html#dyes


QUESTION: Does AMA or methylamine cause any degradation to fluorescein or fluorescein-type dyes such as FAM or FITC?

RESPONSE:Response: While AMA (Ammonium hydroxide/40% Methylamine 1:1 v/v) is considered compatible with fluorescein, the use of methylamine when deprotecting a Fluorescein-labeled oligo does lead to a small amount of degradation, which is characterized by a the appearance of a late-eluting peak by RP HPLC that shows no visible fluorescein absorbance. With standard deprotection conditions (AMA 10 minutes at 65 C) the amount of degradation is approximately 5%. The impurity is not detected with AMA at RT for 2 hours.

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

品牌介绍
Athens Research & Technology—供应多款蛋白、酶制品 Athens Research & Technology是成立于2010年的新兴生物高科技制品公司,该公司拥有BSL-2实验室及超过11000平方英尺的cGMP生产厂房,并且通过ISO 9001:2008认证。Athens公司致力于纯化分离高纯度,高活性的人类蛋白质及研发多克隆抗血清产品。提供包括高纯度及活性的丝氨酸蛋白酶,蛋白酶抑制剂,中性粒细胞酶,载脂蛋白,脂蛋白,血小板蛋白,转铁蛋白,免疫球蛋白等等。公司产品适用于炎症,冠状动脉疾病,自身免疫性疾病,癌症,阿尔茨海默氏病等众多研究领域,已被世界*制药公司及诊断试剂公司用于体外诊断试剂盒/免疫检测试剂盒、药物筛选、细胞培养液(包括干细胞)等产品研发。除了人类蛋白质,我们还从动物血清和组织中分离多种蛋白质及酶类。此外,Athens Research and Technology还提供特殊试剂/蛋白定制服务,以满足研究人员的不同需求。