

Oligo Synthesis : CEPs
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EDTA-C2-dT-CE Phosphoramidite
EDTA-C2-dT-CE Phosphoramidite
Glen Research
Catalogue No. | Description | Pack Size | Price | Qty |
|
---|---|---|---|---|---|
10-1059-02 | EDTA-C2-dT-CE Phosphoramidite | 0.25g | £780.00£741.00Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
10-1059-90 | EDTA-C2-dT-CE Phosphoramidite | 100µmoles | £396.00£376.20Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
10-1059-95 | EDTA-C2-dT-CE Phosphoramidite | 50µmoles | £200.00£190.00Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
Related products
EDTA-C2-dT-CE Phosphoramidite
EDTA-C2-dT-CE Phosphoramidite
Glen Research
EDTA-C2-dT-CE Phosphoramidite |
Catalog Number: 10-1059-xx
Description: EDTA-C2-dT-CE Phosphoramidite
5"-Dimethoxytrityl-5-[N-ethylenediaminetriethylacetate,monoacetylaminoethyl-3-acrylimido]-2"-deoxyUridine,3"-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite | ||
Formula: C60H81N8O16P | M.W.: 1201.32 | F.W.: 676.53 |
Diluent: Anhydrous Acetonitrile |
Coupling: 6 minute coupling time recommended. |
Deprotection: 0.4M methanolic sodium hydroxide (methanol:water 4:1) at room temperature for 17 hours.Note: NaOH is not compatible with dmf protecting groups. |
Storage: Refrigerated storage, maximum of 2-8°C, dry |
Stability in Solution: 2-3 days EDTA LABELLINGEDTA-C2-dT phosphoramidite contains the triethyl ester of EDTA which allows sequence-specific cleavage of single- and double-stranded DNA and RNA. The cleavage reaction is only initiated once Fe(II) and dithiothreitol are added and so is readily controlled. Coupling of EDTA-dT is normal but cleavage and deprotection should be carried out with sodium hydroxide in aqueous methanol (0.4M NaOH in methanol/water 4:1) overnight at room temperature. |
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EDTA-C2-dT-CE Phosphoramidite
EDTA-C2-dT-CE Phosphoramidite
Glen Research
Material Safety Data Sheet
Glen Report 11.2: MORE NOVEL MONOMERS |
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
EDTA-C2-dT-CE Phosphoramidite
EDTA-C2-dT-CE Phosphoramidite
Glen Research
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link formore detailed usage informationwith the various synthesizers.
ABI 392/394 | |||||||||
Cat.No. | PackSize | Grams/Pack | 0.1M Dil.(mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
Approximate Number of Additions | |||||||||
10-1059-02 | 0.25grams | .25grams | 2.08 | 56 | 33.6 | 21 | 15.27 | 11.2 | 2.8 |
10-1059-90 | 100µmoles | .12grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
10-1059-95 | 50µmoles | .06grams | .5 | 3.33 | 2 | 1.25 | .91 | .67 | .17 |
Expedite | |||||||||
Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
Approximate Number of Additions | |||||||||
10-1059-02 | 0.25grams | .25grams | 3.11 | .07 | 55.8 | 34.88 | 25.36 | 3.49 | |
10-1059-90 | 100µmoles | .12grams | 1.5 | .07 | 23.6 | 14.75 | 10.73 | 1.48 | |
10-1059-95 | 50µmoles | .06grams | .75 | .07 | 8.6 | 5.38 | 3.91 | .54 | |
Beckman | |||||||||
Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 30nm | 200nm | 1000nm | ||
Approximate Number of Additions | |||||||||
10-1059-02 | 0.25grams | .25grams | 3.11 | .07 | 57.4 | 35.88 | 26.09 | ||
10-1059-90 | 100µmoles | .12grams | 1.5 | .07 | 25.2 | 15.75 | 11.45 | ||
10-1059-95 | 50µmoles | .06grams | .75 | .07 | 10.2 | 6.38 | 4.64 |
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200