Oligo Synthesis : CEPs
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dT-Me Phosphoramidite
dT-Me Phosphoramidite
Glen Research
| Catalogue No. | Description | Pack Size | Price | Qty |
|
|---|---|---|---|---|---|
| 10-1330-02 | dT-Me Phosphoramidite | 0.25g | £30.00£28.50Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 10-1330-05 | dT-Me Phosphoramidite | 0.5g | £40.00£38.00Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 10-1330-10 | dT-Me Phosphoramidite | 1.0g | £80.00£76.00Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
Related products
dT-Me Phosphoramidite
dT-Me Phosphoramidite
Glen Research
| dT-Me Phosphoramidite |
Catalog Number: 10-1330-xx
Description: dT-Me Phosphoramidite
| 5"-Dimethoxytrityl-2"-deoxyThymidine,3"-[(methyl)-(N,N-diisopropyl)]-phosphoramidite | ||
| Formula: C38H48N3O8P | M.W.: 705.79 | F.W.: 318.22 (Methyl triester) |
Diluent: Anhydrous Acetonitrile |
| Coupling: No changes needed from standard method recommended by synthesizer manufacturer. To avoid any exchange of the iPr-Pac group on the dG with acetyl, use the UltraMild Cap Mix A (40-4210-xx/ 40-4212-xx). |
| Deprotection: UltraMILD deprotection: 0.05M Potassium Carbonate in Methanol, 4 hours at Room Temperature to leave the methyl phosphotriester intact.Technical Bulletin |
| Storage: Refrigerated storage, maximum of 2-8°C, dry |
| Stability in Solution: 24 hours |
METHYL PHOSPHORAMIDITES
For many years, Glen Research has supplied methyl phosphoramidites in addition to þ-cyanoethyl (CE) phosphoramidites for the few situations where the more labile cyanoethyl group is not an advantage. Some of our customers, probably remembering that the methyl group was removed specifically with thiophenol, have tried to use these monomers to prepare the interesting, uncharged, and nuclease-resistant methyl phosphotriester linkage. Unfortunately, this linkage is labile to ammonium hydroxide and the regular phosphodiester linkage is formed (along with a small amount of chain scission). Now we offer UltraMild methyl phosphoramidites for this application. Oligos produced from these monomers can be deprotected with potassium carbonate in methanol to produce methyl phosphotriester linkages. Since these linkages are diastereomeric and uncharged, the oligos may be hard to handle. Consequently, it is likely that chimeras will be produced using these monomers along with the regular UltraMild CE phosphoramidites. If many dG residues are included in the oligonucleotide, we recommend the use of phenoxyacetic anhydride (PAc2O) in Cap A. This modification removes the possibility of exchange of the isopropyl-phenoxyacetate (iPr-Pac) protecting group on the dG with acetate from the acetic anhydride capping mix.
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
dT-Me Phosphoramidite
dT-Me Phosphoramidite
Glen Research
Material Safety Data Sheet
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
dT-Me Phosphoramidite
dT-Me Phosphoramidite
Glen Research
REQUENTLY ASKED TECHNICAL QUESTION
QUESTION: Can Me phosphoramidites (10-1300, etc.) be used to produce methyl triester oligonucleotides?
RESPONSE:Possibly, but we are unaware of a method. Some people remember that the methyl phosphoramidites were used to prepare oligos originally and that thiophenol was used to remove the methyl group prior to ammonium hydroxide deprotection. This might suggest that omitting the thiophenol step would lead to the methyl triesters. Wrong. Thiophenol was used to remove the methyl group specifically prior to the ammonium hydroxide step to avoid chain scission. If the methyl triesters are treated with ammonium hydroxide, they are predominantly hydrolyzed to the phosphodiesters but a small percentage of the linkages are also hydrolyzed to eliminate either the 3"or 5" alcohol (chain scission).
While working with dT-Me Phosphoramidite and deprotecting under UltraMild conditions, it was clear that the methyl triester group was essentially unreacted over 24 hours in potassium carbonate in methanol. We have now introduced UltraMild Me Phosphoramidite monomers and, finally, methyl triester oligonucleotides can be simply produced.
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
dT-Me Phosphoramidite
dT-Me Phosphoramidite
Glen Research
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link formore detailed usage informationwith the various synthesizers.
| ABI 392/394 | |||||||||
| Cat.No. | PackSize | Grams/Pack | 0.1M Dil.(mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 10-1330-02 | 0.25grams | .25grams | 3.54 | 104.67 | 62.8 | 39.25 | 28.55 | 20.93 | 5.23 |
| 10-1330-05 | 0.5grams | .5grams | 7.08 | 222.67 | 133.6 | 83.5 | 60.73 | 44.53 | 11.13 |
| 10-1330-10 | 1.0gram | 1grams | 14.17 | 459 | 275.4 | 172.13 | 125.18 | 91.8 | 22.95 |
| Expedite | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 10-1330-02 | 0.25grams | .25grams | 5.29 | .07 | 99.4 | 62.13 | 45.18 | 6.21 | |
| 10-1330-05 | 0.5grams | .5grams | 10.57 | .07 | 205 | 128.13 | 93.18 | 12.81 | |
| 10-1330-10 | 1.0gram | 1grams | 21.15 | .07 | 416.6 | 260.38 | 189.36 | 26.04 | |
| Beckman | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 10-1330-02 | 0.25grams | .25grams | 5.29 | .07 | 101 | 63.13 | 45.91 | ||
| 10-1330-05 | 0.5grams | .5grams | 10.57 | .07 | 206.6 | 129.13 | 93.91 | ||
| 10-1330-10 | 1.0gram | 1grams | 21.15 | .07 | 418.2 | 261.38 | 190.09 | ||
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
